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density gradient centrifugation  (PromoCell)


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    Structured Review

    PromoCell density gradient centrifugation
    Density Gradient Centrifugation, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/density+gradient+separating+medium/Lymphocyte+Separation+Medium+1077/pm41610918-43-29-36
    Average 96 stars, based on 148 article reviews
    density gradient centrifugation - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance.
    Article Snippet: .. PBMCs from buffy coats were purified by Ficoll density gradient centrifugation (Lymphocyte Separation Medium 1077, Promocell, C-44010) and cryopreserved in FCS containing 10% DMSO at −80°C. ..

    Gradient Centrifugation:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance.
    Article Snippet: .. PBMCs from buffy coats were purified by Ficoll density gradient centrifugation (Lymphocyte Separation Medium 1077, Promocell, C-44010) and cryopreserved in FCS containing 10% DMSO at −80°C. ..

    Article Title: Proprotein convertase subtilisin/kexin type 9–CC-motif chemokine ligand 2 interactions link lipoprotein(a) composition to intermediate monocyte inflammation in coronary artery disease
    Article Snippet: .. Cells were isolated by density gradient centrifugation using Lymphocyte Separation Medium 1077 (Promocell, Heidelberg, Germany) and SepMate-15 Tubes (Stemcell, Vancouver, Canada). .. After washing, the PBMCs were resuspended in 90% FBS Gold (Seraglob, Schaffhausen, Switzerland) supplemented with 10% dimethyl sulfoxide (DMSO, >99.9% purity, Sigma–Aldrich, St. Louis, USA) and frozen at −80 °C in a CoolCell reduced-scale freezing container (Corning, Corning, USA).

    Article Title: Lanostane-type triterpenoids from ethnomedicinal fungi inhibit endothelial cell proliferation.
    Article Snippet: Ethnopharmacological relevance: Ethnomedicinal fungi have a long history of use in the treatment of tumors and inflammatory disorders, pathologies often associated with dysregulated angiogenesis and immune-vascular interactions.. However, their effects on endothelial cell functions relevant to angiogenesis and inflammation remain insufficiently characterized.. Aim of the study: The aim of this study was to identify the bioactive compounds from ethnopharmacologically relevant medicinal fungi by screening their ethanolic extracts for their effects on angiogenesisand inflammation-related endothelial cell functions, followed by the identification and targeted isolation of these compounds using molecular networking and bioactivity-guided fractionation.

    Article Title: Extracellular vesicles derived from Enterococcus faecalis: inflammatory activation does not require internalization.
    Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using lymphocyte separation medium 1077 (cat. no. C-44010, PromoCell) and LeucoSep tubes (cat. no. 227290, Greiner). .. Monocytes were obtained by magnetic cell sorting using anti-CD14 microbeads (cat. no. 130-050-201, Miltenyi).

    Isolation:

    Article Title: Proprotein convertase subtilisin/kexin type 9–CC-motif chemokine ligand 2 interactions link lipoprotein(a) composition to intermediate monocyte inflammation in coronary artery disease
    Article Snippet: .. Cells were isolated by density gradient centrifugation using Lymphocyte Separation Medium 1077 (Promocell, Heidelberg, Germany) and SepMate-15 Tubes (Stemcell, Vancouver, Canada). .. After washing, the PBMCs were resuspended in 90% FBS Gold (Seraglob, Schaffhausen, Switzerland) supplemented with 10% dimethyl sulfoxide (DMSO, >99.9% purity, Sigma–Aldrich, St. Louis, USA) and frozen at −80 °C in a CoolCell reduced-scale freezing container (Corning, Corning, USA).

    Article Title: Extracellular vesicles derived from Enterococcus faecalis: inflammatory activation does not require internalization.
    Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using lymphocyte separation medium 1077 (cat. no. C-44010, PromoCell) and LeucoSep tubes (cat. no. 227290, Greiner). .. Monocytes were obtained by magnetic cell sorting using anti-CD14 microbeads (cat. no. 130-050-201, Miltenyi).

    Saline:

    Article Title: Short-interval longitudinal analysis of immune cell dynamics in heavy draft foals after colostrum intake.
    Article Snippet: .. The buffy coat 132 was collected, diluted with phosphate-buffered saline (PBS; pH 7.4), and layered over 4.5 mL of 133 Lymphocyte Separation Medium 1077 (PromoCell, Heidelberg, Germany). ..



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