lymphocyte separation medium (Cytiva Europe)

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Cytiva Europe lymphocyte separation medium
Lymphocyte Separation Medium, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+separating+medium/Ficoll-Paque+PREMIUM+density+gradient+media/pm41128463-185-8-12
Average 95 stars, based on 382 article reviews

lymphocyte separation medium - by Bioz Stars, 2026-09

95/100 stars

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Suspension:

Article Title: Whole genome methylation profiling of menstrual stem cells identifies novel biomarkers for endometriosis.
Article Snippet: Briefly, menstrual blood in collection medium was washed with 1x PBS (Gibco) and filtered to remove blood clots and tissue. .. The filtered suspension was slowly overlaid on Ficoll-Paque PREMIUM (Cytiva) and mononuclear cells were isolated by density gradient centrifugation21. .. Flow cytometry and MenSC isolation by FACS Menstrual blood derived-mononuclear cells prepared as described above were washed and counted on a MACSQuant Analyzer 10 (Miltenyi Biotec).

Isolation:

Article Title: HIV tri-specific killer engagers directly enhance NK cell function and safely expand NK cells in vivo.
Article Snippet: The purity of the TriKE protein was validated using SDS PAGE gel stained with One-Step Blue (Biotium, Cat# 21003-1L), and protein was quantified using Qubit Protein Assay kit (Invitrogen, Cat# Q33211). .. Peripheral blood mononuclear cells and NK cell isolation Peripheral blood mononuclear cells (PBMC) were isolated from Leukopaks for PWOH (n = 5) and EDTA tubes for PWH (n = 5), using density-gradient centrifugation with Ficoll-Paque Premium (GE Healthcare, Cat. No. GE17-5442-03). .. NK cells were enriched from PBMCs using negative selection magnetic beads from EasySep Human NK Cell Enrichment kit (STEMCELL Technologies, Cat. No. 19055).

Article Title: TTV Viremia and Immune Responses Following Vaccination Against Mpox and Dengue Viruses
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood samples collected in heparinized tubes using density gradient centrifugation with Ficoll-Paque Premium (GE Healthcare Life Sciences, Chicago, IL, USA). .. The isolated PBMCs were cryopreserved in fetal bovine serum (FBS; Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10% dimethyl sulfoxide (DMSO; Gibco, Life Technologies, Carlsbad, CA, USA) and stored in liquid nitrogen until further use.

Article Title: TRIM28-mediated SUMOylation of SREBF1 drives PD‑L1 N‑glycosylation and immune evasion in bladder cancer.
Article Snippet: .. Isolation of PBMCs and CD8+ T cells Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Paque density gradient centrifugation (GE Healthcare, Cat# 17-5442-02), followed by two PBS washes to remove platelets and impurities. .. CD8+ T cells were purified using a CD8+ T Cell Isolation Kit (Miltenyi Biotec, Cat# 130-096-495).

Cell Isolation:

Centrifugation:

Recombinant:

Article Title: Salmonella vector creates de novo parvovirus that reduces solid tumors and forms antitumor immune memory.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-NS1 Majumder et al.41 N/A HRP-conjugated goat anti-mouse IgG R&D Systems Cat# HAF007; RRID: AB_357234 FITC-conjugated rabbit anti-Salmonella polyclonal Abcam Cat# ab69253; RRID: AB_1270507 Rat anti-myc monoclonal Chromotek Cat# 9e1-100; RRID: AB_2631398 Alexa Fluor 568 goat anti-rat secondary Thermo Fisher Cat# A11077; RRID: AB_2534121 DAPI-containing mountant Thermo Fisher Cat# P36962 Rabbit polyclonal anti-NS1 Dr. Jürg Nüesh N/A Rabbit polyclonal anti-VP Dr. Jürg Nüesh N/A HRP-conjugated goat anti-rabbit IgG Invitrogen Cat# 31466; RRID: AB_2533967 Anti-mouse CD16/32 Biolegend Cat# 101302; RRID: AB_312800 APC anti-mouse CD45 Biolegend Cat# 157605; RRID: AB_2876537 APC/Cyanine7 anti-mouse F4/80 Biolegend Cat# 123117; RRID: AB_893489 Brilliant Violet 421TM anti-mouse CD3 Biolegend Cat# 100227; RRID: AB_10900227 Alexa Fluor® 488 anti-mouse CD8a Biolegend Cat# 100726; RRID: AB_493423 Brilliant Violet 605TM anti-mouse CD80 Biolegend Cat# 104729; RRID: AB_11126141 Brilliant Violet 421TM anti-mouse CD335 (NKp46) Biolegend Cat# 137611; RRID: AB_2563104 FITC anti-mouse CD11c Biolegend Cat# 117305; RRID: AB_313774 PE/DazzleTM 594 anti-mouse CD206 (MMR) Biolegend Cat# 141731; RRID: AB_2565931 APC/Cyanine7 anti-mouse CD4 Biolegend Cat# 100414; RRID: AB_312699 Brilliant Violet 605TM anti-mouse CD25 Biolegend Cat# 102035; RRID: AB_11126977 APC anti-mouse CD8a Biolegend Cat# 100711; RRID: AB_312750 APC/Cyanine7 anti-mouse CD45 Biolegend Cat# 113115; RRID: AB_312980 FITC anti-mouse CD44 Biolegend Cat# 156007; RRID: AB_2941437 Brilliant Violet 570TM anti-mouse F4/80 Biolegend Cat# 123171; RRID: AB_3106088 PE/Cyanine5 anti-mouse CD80 Biolegend Cat# 104711; RRID: AB_313132 Brilliant Violet 785TM anti-mouse CD11c Biolegend Cat# 117335; RRID: AB_11219204 (Continued on next page) Cell Reports Medicine 7, 102839, June 16, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse CD8a Biolegend Cat# 100707; RRID: AB_312746 Bacterial strains E. coli SURE 2 Agilent 200152 Salmonella enterica serovar Typhimurium (VNP20009) Vion Pharmaceuticals, Inc. N/A Salmonella strain CDS This paper N/A Salmonella strain BB’CDF Khanduja et al.82 N/A Salmonella strain BB’CDF-S This paper N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Cytiva SH3091003 Dulbecco’s modified Eagle medium-low glucose Sigma Aldrich D5523-10X1L Minimum essential medium (MEM) Themo Scientific Cat# 11095080 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide Themo Fisher Cat# M6494 Ficoll Paque premium Cytiva Cat# 17544202 Kanamycin sulfate Fisher Scientific Cat# J17924.06 Carbenicillin disodium salt Chem-Impex Cat# 00049 Gentamicin sulfate Fisher Scientific Cat# BP918-1 Chloramphenicol Fisher Scientific Cat# 227921000 SSPI-HF restriction enzyme New England Biolabs Cat# R3132S Laemmli SDS sample buffer, reducing (6X) Fisher Scientific Cat# J61337.AC EcoRI-HF restriction enzyme New England Biolabs Cat# R3101S Ampicillin sodium salt Fisher Scientific Cat# A0166 2,6-Diaminopimelic acid (DAP) Thermo Scientific Cat# B22391.06 Immobilon Western Chemiluminescent HRP Substrate Millipore Sigma WBKLS Phenylmethanesulfonyl fluoride (PMSF) Thermo Scientific Cat# 215740500 Collagenase D Millipore sigma Cat# 11088866001 Benzonase Millipore sigma 9025-65-4 Critical commercial assays Lipofectamine 3000 Transfection Reagent Fisher Scientific Cat# L3000008 Zyppy plasmid miniprep kit Zymo Cat# D4037 Deposited data Raw and analyzed data This paper Mendeley Data : https://doi.org/10.17632/t7ppsjcgw4.1 Experimental models: cell lines Human embryonic kidney cells (HEK-293T) ATCC Cat# CRL-3216; RRID: CVCL_0063 Human hepatocellular carcinoma cells (HuH7) Cytion Cat# 300156; RRID: CVCL_0336 Murine pancreatic ductal adenocarcinoma cells (KPCY) Kerafast Cat# EUP001-FP; RRID: CVCL_YM32 Human hepatocellular carcinoma cells (Hep3B) ATCC Cat# HB-8064; RRID: CVCL_0326 Human liver adenocarcinoma (Sk-Hep-1) ATCC Cat# HTB-52; RRID: CVCL_0525 Mouse hepatoma cells (Hepa1-6) ATCC Cat# CRL-1830; RRID: CVCL_0327 (Continued on next page) e2 Cell Reports Medicine 7, 102839, June 16, 2026 Article ll OPEN ACCESS .. Seven mammalian cell lines were used throughout: HEK-293T human embryonic kidney cells (RRID:CVCL_0063; ATCC, Manassas, VA), HuH7 human hepatocellular carcinoma cells (RRID:CVCL_0336; Cytion), Hep3B human hepatocellular carcinoma cells (RRID: CVCL_0326; ATCC, Manassas, VA), Sk-Hep-1 human liver adenocarcinoma (RRID: CVCL_0525; ATCC, Manassas, VA), Hepa1-6 mouse hepatoma cells (RRID:CVCL_0327; ATCC, Manassas, VA), MCF-7 human breast adenocarcinoma cells (RRID:CVCL_3397; ATCC, Manassas, VA), and KPCY murine pancreatic ductal adenocarcinoma cells (RRID:CVCL_YM32; Kerafast, Boston, MA).

Modification:

MTT Assay:

Western Blot:

Transfection:

Plasmid Preparation:

Gradient Centrifugation:

Saline:

Article Title: Bone Marrow Mononuclear Cells and Plasma Gel as Combination Treatment for Hard-to-Heal Wounds
Article Snippet: .. Bone marrow mononuclear cells were isolated using Ficoll-PaqueTM PREMIUM density gradient centrifugation (1.077 g/mL; Cytiva, Marlborough, MA, USA) at 400× g for 25 min at 20 °C, washed in saline (0.9%; Grifols, Barcelona, Spain), and counted. .. The plasma scaffold was prepared in a 6-well tissue culture plate (Avantor, Radnor, PA, USA) by mixing 3 mL of plasma with one million cells per cm 2 and adding 60 μL of tranexamic acid (Amchafibrin 100 mg/mL, Viatris, Pittsburgh, PA, USA).

Article Title: Distinct remission immune architectures under rituximab and azathioprine in AQP4-IgG-positive neuromyelitis optica spectrum disorder
Article Snippet: .. Whole blood was diluted with phosphate-buffered saline (PBS) and layered onto Ficoll-Paque Premium (Cytiva) for density gradient separation. ..

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