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910a t densitometry analysis  (Bethyl)


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    Structured Review

    Bethyl 910a t densitometry analysis
    910a T Densitometry Analysis, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+analysis/Sin1+Antibody/pmc12895852-417-7-5
    Average 93 stars, based on 32 article reviews
    910a t densitometry analysis - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Control:

    Article Title: Mechanosensitive mTORC2 independently coordinates leading and trailing edge polarity programs during neutrophil migration
    Article Snippet: Analysis was performed using Image Studio (LI-COR) and Excel. .. The following primary antibodies were used for the study: phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) (Cell Signaling; #2605), PAK2 (3B5) (Cell Signaling; #4825), phospho-Akt (Ser473; D9E) XP (Cell Signaling; #4060), Akt (pan; 40D4; Cell Signaling #2920S), Rictor (Bethyl; #A300-458A), mSin1 (Bethyl; #A300-910A), and GAPDH Loading Control Antibody GA1R (Thermo Fisher). .. Secondary antibodies IRDye 680RD goat antimouse (LI-COR) and IRDye 800CW goat anti-rabbit (LI-COR) were used.

    Software:

    Article Title: The mTORC2 component SIN1 post-transcriptionally regulates TYMS levels and modulates P53 activity in response to 5-FU chemotherapy
    Article Snippet: The following antibodies were purchased from Invitrogen: phospho-AKT1 (Ser473) # 700256; AKT Pan # MA5–14916, Phospho-p70 S6 Kinase (Thr389) #710095, P70 S6 Kinase #MA5-15141, P21 Monoclonal Antibody (R.229.6) # MA5-14949, GAPDH # MA5-15738. .. SIN1 antibody was purchased from Bethyl Laboratories #A300-910A-T. Densitometry analysis of proteins was performed using ImageJ quantification software to measure the relative band intensity. ..

    Western Blot:

    Article Title: c-JUN inhibits mTORC2 and glucose uptake to promote self-renewal and obesity.
    Article Snippet: .. Western blot analysis was carried, using antibodies against C-Jun and Rictor (Santa Cruz Biotechnology Inc); FOXO1, FOXO1S256, AKT, AKTS473, AKTT308, m TOR, GSK and P-GSK (Cell Signaling Technology); and Sin1 (Bethyl Laboratories). ..

    Knock-Out:

    Article Title: Structural Basis for the Recruitment and Selective Phosphorylation of Akt by mTORC2
    Article Snippet: Mouse monoclonal to vinculin was from Santa Cruz (sc-73614). .. Mouse monoclonal to mSin1 (Sigma 05–1044, clone 1C7.2) was knockout validated; Rabbit polyclonal to mSin1 (Bethyl A300–910A) was used for insect overexpression but did not detect endogenous mSin1 in HEK-293T or HCT116. p-Ser916 PKN1 antibody and non-phospho PKN1 blocking peptide were gifts from Bryce Paschal and used as described( 28 ). ..

    Over Expression:

    Article Title: Structural Basis for the Recruitment and Selective Phosphorylation of Akt by mTORC2
    Article Snippet: Mouse monoclonal to vinculin was from Santa Cruz (sc-73614). .. Mouse monoclonal to mSin1 (Sigma 05–1044, clone 1C7.2) was knockout validated; Rabbit polyclonal to mSin1 (Bethyl A300–910A) was used for insect overexpression but did not detect endogenous mSin1 in HEK-293T or HCT116. p-Ser916 PKN1 antibody and non-phospho PKN1 blocking peptide were gifts from Bryce Paschal and used as described( 28 ). ..

    Blocking Assay:

    Article Title: Structural Basis for the Recruitment and Selective Phosphorylation of Akt by mTORC2
    Article Snippet: Mouse monoclonal to vinculin was from Santa Cruz (sc-73614). .. Mouse monoclonal to mSin1 (Sigma 05–1044, clone 1C7.2) was knockout validated; Rabbit polyclonal to mSin1 (Bethyl A300–910A) was used for insect overexpression but did not detect endogenous mSin1 in HEK-293T or HCT116. p-Ser916 PKN1 antibody and non-phospho PKN1 blocking peptide were gifts from Bryce Paschal and used as described( 28 ). ..



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    Image Search Results


    Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Staining, Western Blot, Marker, Control

    ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Western Blot, Control

    Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Staining, Western Blot, Marker, Control