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amershamtm 600 imager imaging system  (Bio-Rad)


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    Bio-Rad amershamtm 600 imager imaging system
    Amershamtm 600 Imager Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+analysis+software+imagequant+program+version+3%2E3/Lyphochek+Hypertension+Markers+Control/pmc08267626-170-6-31
    Average 88 stars, based on 9 article reviews
    amershamtm 600 imager imaging system - by Bioz Stars, 2026-10
    88/100 stars

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    Related Articles

    Spectrophotometry:

    Article Title: The effect of DNA-binding proteins on insertion sequence element transposition upstream of the bgl operon in Escherichia coli
    Article Snippet: .. The tubes were rotated at 250 rpm and 37°C, and cell densities (OD 600 ) were measured with a Bio-Rad spectrophotometer. ..

    Article Title: Overexpressing the cpr1953 Orphan Histidine Kinase Gene in the Absence of cpr1954 Orphan Histidine Kinase Gene Expression, or Vice Versa, Is Sufficient to Obtain Significant Sporulation and Strong Production of Clostridium perfringens Enterotoxin or Spo0A by Clostridium perfringens Type F Strain SM101
    Article Snippet: .. The cultures were incubated anaerobically at 37 °C for 0, 2, 4, 6, 8, or 24 h. Then, a 1 mL aliquot culture of each isolate was collected and OD 600 was measured using a Bio-Rad SmartSpec spectrophotometer (Hercules, CA, USA). ..

    Article Title: High-resolution epitope mapping of commercial antibodies to ANCA antigens by yeast surface display.
    Article Snippet: While the beads incubated with antibody, we pelleted 5 × 106 yeast cells that had been induced to express the surface displayed peptide (cultured overnight on SGCAA media (Teknova) to drive peptide expression via their galactose-inducible promoter). .. Density of the yeast cultures was determined by measuring their optical density at 600 nm (OD600) on a spectrophotometer (BioRad). ..

    Article Title: Overexpressing the cpr1953 Orphan Histidine Kinase Gene in the Absence of cpr1954 Orphan Histidine Kinase Gene Expression, or Vice Versa, Is Sufficient to Obtain Significant Sporulation and Strong Production of Clostridium perfringens Enterotoxin or Spo0A by Clostridium perfringens Type F Strain SM101
    Article Snippet: .. Each C. perfringens culture was then adjusted to equal OD 600 using a Bio-Rad SmartSpec spectrophotometer. .. Equal volume of those adjusted cultures was harvested by centrifugation, washed with PBS buffer, lysed with B-PER reagent (ThermoFisher Scientific) containing protease inhibitor cocktail II (Research Products International), and then boiled for 5 min at 95 °C.

    Synthesized:

    Article Title: C-C motif chemokine receptor 2 and 7 synergistically control inflammatory monocyte recruitment but the infecting virus dictates monocyte function in the brain
    Article Snippet: 5 μl of brain and 50 μl of lymph node RNA was then DNase treated using the DNase I kit instructions (Ambion DNase). .. DNase-treated samples were then cleaned up with the Zymo RNA cleanup kit following the manufacturer’s instructions, with the exception that RNA wash steps were performed with 600 ul Wash Buffer and RNA was eluted in 50 μl warm NF H O. cDNA was synthesized from the cleaned-up RNA using the BioRad iScript cDNA synthesis kit, following the kit instructions. cDNA samples were diluted five-fold in nuclease-free water and quantitative real-time PCR reactions were set up in triplicate in 384-well plates using SYBR Green SuperMix with ROX (Bio-Rad Laboratories) per the manufacturer’s recommendation with each primer at a 10 mM concentration. cDNA without reverse transcriptase and nuclease-free water with reverse transcriptase were used as negative controls. .. All samples were run on a Viia7 (Applied Biosystems) with a 95 °C dissociation for 3 min followed by 40 cycles of 95 °C/15 s to 60 °C/1 min amplification.

    cDNA Synthesis:

    Article Title: C-C motif chemokine receptor 2 and 7 synergistically control inflammatory monocyte recruitment but the infecting virus dictates monocyte function in the brain
    Article Snippet: 5 μl of brain and 50 μl of lymph node RNA was then DNase treated using the DNase I kit instructions (Ambion DNase). .. DNase-treated samples were then cleaned up with the Zymo RNA cleanup kit following the manufacturer’s instructions, with the exception that RNA wash steps were performed with 600 ul Wash Buffer and RNA was eluted in 50 μl warm NF H O. cDNA was synthesized from the cleaned-up RNA using the BioRad iScript cDNA synthesis kit, following the kit instructions. cDNA samples were diluted five-fold in nuclease-free water and quantitative real-time PCR reactions were set up in triplicate in 384-well plates using SYBR Green SuperMix with ROX (Bio-Rad Laboratories) per the manufacturer’s recommendation with each primer at a 10 mM concentration. cDNA without reverse transcriptase and nuclease-free water with reverse transcriptase were used as negative controls. .. All samples were run on a Viia7 (Applied Biosystems) with a 95 °C dissociation for 3 min followed by 40 cycles of 95 °C/15 s to 60 °C/1 min amplification.

    Real-time Polymerase Chain Reaction:

    Article Title: C-C motif chemokine receptor 2 and 7 synergistically control inflammatory monocyte recruitment but the infecting virus dictates monocyte function in the brain
    Article Snippet: 5 μl of brain and 50 μl of lymph node RNA was then DNase treated using the DNase I kit instructions (Ambion DNase). .. DNase-treated samples were then cleaned up with the Zymo RNA cleanup kit following the manufacturer’s instructions, with the exception that RNA wash steps were performed with 600 ul Wash Buffer and RNA was eluted in 50 μl warm NF H O. cDNA was synthesized from the cleaned-up RNA using the BioRad iScript cDNA synthesis kit, following the kit instructions. cDNA samples were diluted five-fold in nuclease-free water and quantitative real-time PCR reactions were set up in triplicate in 384-well plates using SYBR Green SuperMix with ROX (Bio-Rad Laboratories) per the manufacturer’s recommendation with each primer at a 10 mM concentration. cDNA without reverse transcriptase and nuclease-free water with reverse transcriptase were used as negative controls. .. All samples were run on a Viia7 (Applied Biosystems) with a 95 °C dissociation for 3 min followed by 40 cycles of 95 °C/15 s to 60 °C/1 min amplification.

    Article Title: Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts
    Article Snippet: .. The IDT Primetime gene expression master mix (IDT #1055771) was used for quantitative PCR on a Bio-Rad CFX384 instrument with Taqman probes (1.5 μM for primers; 600 nM probe). ..

    SYBR Green Assay:

    Article Title: C-C motif chemokine receptor 2 and 7 synergistically control inflammatory monocyte recruitment but the infecting virus dictates monocyte function in the brain
    Article Snippet: 5 μl of brain and 50 μl of lymph node RNA was then DNase treated using the DNase I kit instructions (Ambion DNase). .. DNase-treated samples were then cleaned up with the Zymo RNA cleanup kit following the manufacturer’s instructions, with the exception that RNA wash steps were performed with 600 ul Wash Buffer and RNA was eluted in 50 μl warm NF H O. cDNA was synthesized from the cleaned-up RNA using the BioRad iScript cDNA synthesis kit, following the kit instructions. cDNA samples were diluted five-fold in nuclease-free water and quantitative real-time PCR reactions were set up in triplicate in 384-well plates using SYBR Green SuperMix with ROX (Bio-Rad Laboratories) per the manufacturer’s recommendation with each primer at a 10 mM concentration. cDNA without reverse transcriptase and nuclease-free water with reverse transcriptase were used as negative controls. .. All samples were run on a Viia7 (Applied Biosystems) with a 95 °C dissociation for 3 min followed by 40 cycles of 95 °C/15 s to 60 °C/1 min amplification.

    Concentration Assay:

    Article Title: C-C motif chemokine receptor 2 and 7 synergistically control inflammatory monocyte recruitment but the infecting virus dictates monocyte function in the brain
    Article Snippet: 5 μl of brain and 50 μl of lymph node RNA was then DNase treated using the DNase I kit instructions (Ambion DNase). .. DNase-treated samples were then cleaned up with the Zymo RNA cleanup kit following the manufacturer’s instructions, with the exception that RNA wash steps were performed with 600 ul Wash Buffer and RNA was eluted in 50 μl warm NF H O. cDNA was synthesized from the cleaned-up RNA using the BioRad iScript cDNA synthesis kit, following the kit instructions. cDNA samples were diluted five-fold in nuclease-free water and quantitative real-time PCR reactions were set up in triplicate in 384-well plates using SYBR Green SuperMix with ROX (Bio-Rad Laboratories) per the manufacturer’s recommendation with each primer at a 10 mM concentration. cDNA without reverse transcriptase and nuclease-free water with reverse transcriptase were used as negative controls. .. All samples were run on a Viia7 (Applied Biosystems) with a 95 °C dissociation for 3 min followed by 40 cycles of 95 °C/15 s to 60 °C/1 min amplification.

    Reverse Transcription:

    Article Title: C-C motif chemokine receptor 2 and 7 synergistically control inflammatory monocyte recruitment but the infecting virus dictates monocyte function in the brain
    Article Snippet: 5 μl of brain and 50 μl of lymph node RNA was then DNase treated using the DNase I kit instructions (Ambion DNase). .. DNase-treated samples were then cleaned up with the Zymo RNA cleanup kit following the manufacturer’s instructions, with the exception that RNA wash steps were performed with 600 ul Wash Buffer and RNA was eluted in 50 μl warm NF H O. cDNA was synthesized from the cleaned-up RNA using the BioRad iScript cDNA synthesis kit, following the kit instructions. cDNA samples were diluted five-fold in nuclease-free water and quantitative real-time PCR reactions were set up in triplicate in 384-well plates using SYBR Green SuperMix with ROX (Bio-Rad Laboratories) per the manufacturer’s recommendation with each primer at a 10 mM concentration. cDNA without reverse transcriptase and nuclease-free water with reverse transcriptase were used as negative controls. .. All samples were run on a Viia7 (Applied Biosystems) with a 95 °C dissociation for 3 min followed by 40 cycles of 95 °C/15 s to 60 °C/1 min amplification.

    Incubation:

    Article Title: Overexpressing the cpr1953 Orphan Histidine Kinase Gene in the Absence of cpr1954 Orphan Histidine Kinase Gene Expression, or Vice Versa, Is Sufficient to Obtain Significant Sporulation and Strong Production of Clostridium perfringens Enterotoxin or Spo0A by Clostridium perfringens Type F Strain SM101
    Article Snippet: .. The cultures were incubated anaerobically at 37 °C for 0, 2, 4, 6, 8, or 24 h. Then, a 1 mL aliquot culture of each isolate was collected and OD 600 was measured using a Bio-Rad SmartSpec spectrophotometer (Hercules, CA, USA). ..

    Article Title:
    Article Snippet: .. The inset on the right side represent a fluorescent image of the wells with compound 12 and a given oligonucleotide after UV-light excitation taken with the Bio-Rad ChemiDocTM imaging system. (B) Emission at 600 nm of compound 12 after incubation with serial dilutions of oligonucleotides (ss G4 LTR wild type, G4 c-myc and G4 c-kit1) spanning final concentrations 2 to 0.002 μM after excitation at 280 nm. ..

    Imaging:

    Article Title:
    Article Snippet: .. The inset on the right side represent a fluorescent image of the wells with compound 12 and a given oligonucleotide after UV-light excitation taken with the Bio-Rad ChemiDocTM imaging system. (B) Emission at 600 nm of compound 12 after incubation with serial dilutions of oligonucleotides (ss G4 LTR wild type, G4 c-myc and G4 c-kit1) spanning final concentrations 2 to 0.002 μM after excitation at 280 nm. ..

    Gene Expression:

    Article Title: Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts
    Article Snippet: .. The IDT Primetime gene expression master mix (IDT #1055771) was used for quantitative PCR on a Bio-Rad CFX384 instrument with Taqman probes (1.5 μM for primers; 600 nM probe). ..



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