Electrophoresis:
other:
Stripping Membranes:Article Title: NKG2A inhibition promotes NK cell-CD8 + T cell interactions to improve anticancer immunity in ovarian carcinoma.
Article Snippet: .. Briefly, sections were first subjected to stripping using an elution buffer containing 2-mercaptoethanol (M3148, Sigma) and SDS (1610302, Bio-Rad) in a shaking water bath pre-heated to 56°C for 30 min, followed by thorough washing in distilled water. .. Antigen retrieval was performed in Target Retrieval Solution pH 8.0 (Leica) using a microwave, where slides were brought to boiling at full power (approximately 45–90 AR TI CL E IN P RE SS LANICKOVA et al seconds) and maintained at reduced power (20%) for 30 min. Endogenous peroxidase activity was blocked using Bloxall solution (SP-6000-100, Vector) for 10 min, followed by incubation in Blocking Buffer from the Tyramide SuperBoost kit (Thermo Fisher Scientific).
Nucleic Acid Electrophoresis:Article Title: Therapeutic effects of pomegranate hot-water extract via inhibition of apoptosis and oxidative stress in a DHEA-induced mouse model of PCOS
Article Snippet: .. The samples were loaded in SDS-polyacrylamide gel electrophoresis, the protein amounts were normalized using a Bradford protein assay (Bio-rad, Hercules, CA, USA), and protein bands were transferred to polyvinylidene difluoride (PVDF) membranes as reported previously., , After blocking the membranes with 3 % BSA, target antibodies diluted with 0.01 % Tween®-20 dissolved in 0.1 % Tris buffered saline (TBST) were applied to the membranes overnight. .. The samples were loaded in SDS-polyacrylamide gel electrophoresis, the protein amounts were normalized using a Bradford protein assay (Bio-rad, Hercules, CA, USA), and protein bands were transferred to polyvinylidene difluoride (PVDF) membranes as reported previously., , After blocking the membranes with 3 % BSA, target antibodies diluted with 0.01 % Tween-20 dissolved in 0.1 % Tris buffered saline (TBST) were applied to the membranes overnight.
Bradford Protein Assay:Article Title: Therapeutic effects of pomegranate hot-water extract via inhibition of apoptosis and oxidative stress in a DHEA-induced mouse model of PCOS
Article Snippet: .. The samples were loaded in SDS-polyacrylamide gel electrophoresis, the protein amounts were normalized using a Bradford protein assay (Bio-rad, Hercules, CA, USA), and protein bands were transferred to polyvinylidene difluoride (PVDF) membranes as reported previously., , After blocking the membranes with 3 % BSA, target antibodies diluted with 0.01 % Tween®-20 dissolved in 0.1 % Tris buffered saline (TBST) were applied to the membranes overnight. .. The samples were loaded in SDS-polyacrylamide gel electrophoresis, the protein amounts were normalized using a Bradford protein assay (Bio-rad, Hercules, CA, USA), and protein bands were transferred to polyvinylidene difluoride (PVDF) membranes as reported previously., , After blocking the membranes with 3 % BSA, target antibodies diluted with 0.01 % Tween-20 dissolved in 0.1 % Tris buffered saline (TBST) were applied to the membranes overnight.
Blocking Assay:Article Title: Therapeutic effects of pomegranate hot-water extract via inhibition of apoptosis and oxidative stress in a DHEA-induced mouse model of PCOS
Article Snippet: .. The samples were loaded in SDS-polyacrylamide gel electrophoresis, the protein amounts were normalized using a Bradford protein assay (Bio-rad, Hercules, CA, USA), and protein bands were transferred to polyvinylidene difluoride (PVDF) membranes as reported previously., , After blocking the membranes with 3 % BSA, target antibodies diluted with 0.01 % Tween®-20 dissolved in 0.1 % Tris buffered saline (TBST) were applied to the membranes overnight. .. The samples were loaded in SDS-polyacrylamide gel electrophoresis, the protein amounts were normalized using a Bradford protein assay (Bio-rad, Hercules, CA, USA), and protein bands were transferred to polyvinylidene difluoride (PVDF) membranes as reported previously., , After blocking the membranes with 3 % BSA, target antibodies diluted with 0.01 % Tween-20 dissolved in 0.1 % Tris buffered saline (TBST) were applied to the membranes overnight.
Saline:Article Title: Therapeutic effects of pomegranate hot-water extract via inhibition of apoptosis and oxidative stress in a DHEA-induced mouse model of PCOS
Article Snippet: .. The samples were loaded in SDS-polyacrylamide gel electrophoresis, the protein amounts were normalized using a Bradford protein assay (Bio-rad, Hercules, CA, USA), and protein bands were transferred to polyvinylidene difluoride (PVDF) membranes as reported previously., , After blocking the membranes with 3 % BSA, target antibodies diluted with 0.01 % Tween®-20 dissolved in 0.1 % Tris buffered saline (TBST) were applied to the membranes overnight. .. The samples were loaded in SDS-polyacrylamide gel electrophoresis, the protein amounts were normalized using a Bradford protein assay (Bio-rad, Hercules, CA, USA), and protein bands were transferred to polyvinylidene difluoride (PVDF) membranes as reported previously., , After blocking the membranes with 3 % BSA, target antibodies diluted with 0.01 % Tween-20 dissolved in 0.1 % Tris buffered saline (TBST) were applied to the membranes overnight.
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