Immunoprecipitation:Article Title: The nuclear Argonaute HRDE-1 broadly interacts with euchromatin via an RGG domain to enable transgenerational epigenetic silencing
Article Snippet: .. To elute the immunoprecipitated proteins and reverse crosslinking, the beads were incubated with 30ul of 2x Laemmli Sample Buffer (BioRad) containing 5% β-mercaptoethanol.at 95°C for 10 mins. .. Protein samples were separated on a 4-20% Mini-Protean TGX Precast Protein Gel (BioRad) at 200V for 50mins and then transferred to a 0.45 μm nitrocellulose membrane (BioRad) at 25V for 6mins using the Trans-blot Turbo Transfer System (Biorad).
Incubation:Article Title: The nuclear Argonaute HRDE-1 broadly interacts with euchromatin via an RGG domain to enable transgenerational epigenetic silencing
Article Snippet: .. To elute the immunoprecipitated proteins and reverse crosslinking, the beads were incubated with 30ul of 2x Laemmli Sample Buffer (BioRad) containing 5% β-mercaptoethanol.at 95°C for 10 mins. .. Protein samples were separated on a 4-20% Mini-Protean TGX Precast Protein Gel (BioRad) at 200V for 50mins and then transferred to a 0.45 μm nitrocellulose membrane (BioRad) at 25V for 6mins using the Trans-blot Turbo Transfer System (Biorad).
Western Blot:Article Title: TET2 loss triggers ssDNA gap accumulation and heightened PARP inhibitor sensitivity.
Article Snippet: 25 μg of protein lysates were mixed with 1X NuPAGE LDS sample buffer (Thermo Fisher Scientific, NP0007) and 200mM DTT, volume made up to 20 μl and proteins were denatured at 95 °C for 5min. .. For TET1 or TET2 western blot, cell pellet was resuspended in 2X laemmli buffer (biorad, #1610737EDU) with 1% beta-mercaptoethanol and was boiled at 95 °C for 45min to lyse the cells completely. .. Proteins were loaded onto a NuPAGE 4–12% Bis-Tris Gel (Thermo Fisher Scientific, NP0322BOX) and run with 1X NuPAGE MES SDS Running buffer (Thermo Fisher Scientific, NP0002) or a or a NuPAGE 3–8% TrisAcetate Gel (Thermo Fisher Scientific, EA0375BOX) and run with 1X NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific, LA0041).
Staining:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
Propidium Iodide Staining:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
Crocin Bleaching Assay:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
Enzyme-linked Immunosorbent Assay:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
Virus:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
CCK-8 Assay:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
Protein Concentration:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
BIA-KA:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
Purification:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
Quantitative RT-PCR:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
Synthesized:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026 .. Cell lines and culture conditions ARPE-19 cells (CRL-2302) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM: F12 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin solution.
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