deep sequencing (Azenta)
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Deep Sequencing, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deep+sequencing+%26+microarray+core+facility/analysis+deep+sequencing/pm41402316-468-54-58
Average 86 stars, based on 1 article reviews
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Knock-Out:Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways. Article Snippet: Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and deep sequencing (using the GENEWIZ commercial service provided by Azenta Life Sciences). .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways Article Snippet: .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5′-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3′, 5′-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT-3′) and Amplification:Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways. Article Snippet: Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and deep sequencing (using the GENEWIZ commercial service provided by Azenta Life Sciences). .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and Article Title: Computational design of dynamic biosensors for emerging synthetic opioids. Article Snippet: PYR1nita was assembled into pND003 to make pRMC075 by Golden Gate assembly; this plasmid was subsequently transformed into EBY100 S. cerevisiae cells (ATCC Catalog Number MYA-4941TM) 60. .. Transformation efficiencies (Supplementary Table 5) were estimated using serial dilution, and library quality (Supplementary Figure 5) determined by short Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways Article Snippet: .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5′-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3′, 5′-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT-3′) and Polymerase Chain Reaction:Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways. Article Snippet: Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and deep sequencing (using the GENEWIZ commercial service provided by Azenta Life Sciences). .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways Article Snippet: .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5′-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3′, 5′-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT-3′) and Countercurrent Chromatography:Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways. Article Snippet: Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and deep sequencing (using the GENEWIZ commercial service provided by Azenta Life Sciences). .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways Article Snippet: .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5′-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3′, 5′-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT-3′) and Sequencing:Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways. Article Snippet: Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and deep sequencing (using the GENEWIZ commercial service provided by Azenta Life Sciences). .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5’-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3’, 5’-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT3’) and Article Title: Computational design of dynamic biosensors for emerging synthetic opioids. Article Snippet: PYR1nita was assembled into pND003 to make pRMC075 by Golden Gate assembly; this plasmid was subsequently transformed into EBY100 S. cerevisiae cells (ATCC Catalog Number MYA-4941TM) 60. .. Transformation efficiencies (Supplementary Table 5) were estimated using serial dilution, and library quality (Supplementary Figure 5) determined by short Article Title: Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways Article Snippet: .. Successful knock-out was additionally verified by amplification of the region around the first exon of the ERCC4 gene by PCR (primers: 5′-CTG CGA CCC GGA AGA GCT TCC ATG GAG TCA GGG CAG CCG GCT-3′, 5′-CGC AGT GTG AGG GAC CTG CAT CCC CCT GGG GAC CCC TGC CAT CCT TCT CTG TGT-3′) and other:Article Title: Drug-controlled CAR T cells through the regulation of cell-cell interactions. Article Snippet: After indicated selection rounds, we amplified the 301-bp LD3containing region within the CAR gene and sent it for deep Transformation Assay:Article Title: Computational design of dynamic biosensors for emerging synthetic opioids. Article Snippet: PYR1nita was assembled into pND003 to make pRMC075 by Golden Gate assembly; this plasmid was subsequently transformed into EBY100 S. cerevisiae cells (ATCC Catalog Number MYA-4941TM) 60. .. Transformation efficiencies (Supplementary Table 5) were estimated using serial dilution, and library quality (Supplementary Figure 5) determined by short Serial Dilution:Article Title: Computational design of dynamic biosensors for emerging synthetic opioids. Article Snippet: PYR1nita was assembled into pND003 to make pRMC075 by Golden Gate assembly; this plasmid was subsequently transformed into EBY100 S. cerevisiae cells (ATCC Catalog Number MYA-4941TM) 60. .. Transformation efficiencies (Supplementary Table 5) were estimated using serial dilution, and library quality (Supplementary Figure 5) determined by short Purification:Article Title: A temperature-sensitive CRISPR-Cas12a system for sterile insect technique. Article Snippet: PCR products were purified in agarose gel using an NEB Monarch® Spin DNA Gel Extraction Kit (Cat. #T1120S). .. The purified products were sent to the Azenta/Genewiz company for Article Title: A temperature-sensitive CRISPR-Cas12a system for sterile insect technique Article Snippet: PCR products were purified in agarose gel using an NEB Monarch® Spin DNA Gel Extraction Kit (Cat. #T1120S). .. The purified products were sent to the Azenta/Genewiz company for |

