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Vizgen Inc decrosslinking buffer
Decrosslinking Buffer, supplied by Vizgen Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/decrosslink+buffer/buffer+decrosslinking/pmc12551790-559-11-13
Average 86 stars, based on 1 article reviews
decrosslinking buffer - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Formalin-fixed Paraffin-Embedded:

Article Title: MERFISH 2.0, an ultra-sensitive single-cell spatial transcriptomics imaging chemistry across diverse tissue types
Article Snippet: .. Briefly, frozen tissue sections were fixed and permeabilized via 70% ethanol overnight at 4°C, while FFPE tissue sections were deparaffinized by Deparaffinization Buffer (Vizgen 20300112) at 55°C for 5 minutes twice, followed by another 5 minutes at RT. ..

Incubation:

Article Title: STAMP: Single-cell transcriptomics analysis and multimodal profiling through imaging
Article Snippet: STAMPs were then processed following the Formalin-Fixed Paraffin-Embedded Tissue Sample Preparation user guide (Vizgen, 91600112 Rev D), starting from page 28, step II.9. .. Briefly, STAMPs were decrosslinked at 90°C for 15 min using a decrosslinking buffer (Vizgen, 20300115), then washed twice with conditioning buffer (1 min each) followed by a 30-min incubation at 37°C. ..

Article Title: MERFISH 2.0, an ultra-sensitive single-cell spatial transcriptomics imaging chemistry across diverse tissue types
Article Snippet: .. The rehydrated tissue sections were incubated with Decrosslinking Buffer (Vizgen 20300115) at 90°C for 15 minutes and cooled on bench for 5 minutes. .. The permeabilized frozen or decrosslinked FFPE tissue sections were then stained for cell boundary using Vizgen’s Cell Boundary Kit (Vizgen 10400118) following Vizgen’s MERFISH 2.0 Sample Preparation User Guide for Sectioned Tissue Samples.

Article Title: TREM2 macrophages are associated with enhanced response to PD-1 blockade in human hepatocellular carcinoma
Article Snippet: Deparaffinization was performed twice using Deparaffinization Buffer (Vizgen 20300112) at 55°C for 5min, followed by sequential ethanol washes (3x in 100% ethanol for 2min each, then 90% and 70% ethanol for 2min each). .. After rehydration, slides were incubated with Decrosslinking Buffer (Vizgen 20300115) at 90°C for 15min, cooled to RT, and treated with Conditioning Buffer (20300116) at 37°C for 30min. ..

Article Title: MERFISH 2.0, an ultra-sensitive single-cell spatial transcriptomics imaging chemistry across diverse tissue types
Article Snippet: .. The rehydrated tissue sections were incubated with Decrosslinking Buffer (Vizgen 20300115) at 90°C for 15 min and cooled on bench for 5 minutes. .. The decrosslinked tissue sections were then incubated with Conditioning Buffer (Vizgen 20300116) at 37°C for 30 minutes, and Pre-Anchoring Reaction Buffer (Vizgen 20300113) for 2 hours at 37°C.

other:

Article Title: Whole-body single-cell atlas of an adult vertebrate in homeostasis and regeneration
Article Snippet: Samples were either mounted immediately after a brief PBST rinse in ProLong Gold (Invitrogen, Product no: P36930) or were optically cleared to RIMS (EasyIndex, Life Canvas Technologies) for imaging.



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