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elements ar analysis 2 d deconvolution software  (Nikon)


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    Nikon elements ar analysis 2 d deconvolution software
    Elements Ar Analysis 2 D Deconvolution Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 40098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+analysis/NIS-Elements/pmc11484838-122-14-13
    Average 99 stars, based on 40098 article reviews
    elements ar analysis 2 d deconvolution software - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Confocal:

    Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model.
    Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the NIS Elements software (ver. ..

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Microscopy:

    Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model.
    Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the NIS Elements software (ver. ..

    Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway
    Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the NIS elements software (Nikon), and emission at 340/380 nm was calculated. .. Cells were imaged during continuous perfusion with CIB and then challenged with sequential 30-s applications of 15 mM KCl, capsaicin (10 nM; Sigma-Aldrich), and 50 mM KCl, with 3-min intervening washouts with CIB.

    Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring.
    Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20.

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Article Title: Discovery of a Virulent Population of Meloidogyne graminicola Overcoming the Rice Resistance Gene MG1 and Population Genetic Analysis Based on mtCOI Sequences.
    Article Snippet: .. Measurements and photographs were https://doi.org/10.3390/plants15193031 taken with a Nikon ECLIPSE Ni microscope equipped with a Nikon Digital Sight Camera and NIS-Elements BR 4.13.05 software (Nikon, Tokyo, Japan). ..

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Software:

    Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model.
    Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the NIS Elements software (ver. ..

    Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway
    Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the NIS elements software (Nikon), and emission at 340/380 nm was calculated. .. Cells were imaged during continuous perfusion with CIB and then challenged with sequential 30-s applications of 15 mM KCl, capsaicin (10 nM; Sigma-Aldrich), and 50 mM KCl, with 3-min intervening washouts with CIB.

    Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring.
    Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20.

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Article Title: Discovery of a Virulent Population of Meloidogyne graminicola Overcoming the Rice Resistance Gene MG1 and Population Genetic Analysis Based on mtCOI Sequences.
    Article Snippet: .. Measurements and photographs were https://doi.org/10.3390/plants15193031 taken with a Nikon ECLIPSE Ni microscope equipped with a Nikon Digital Sight Camera and NIS-Elements BR 4.13.05 software (Nikon, Tokyo, Japan). ..

    Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut
    Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon ECLIPSE Ti2 inverted microscope, NiS‐Elements D, version 5.30.02 software). .. Brightfield images of the gut were overlaid with fluorescent images to accurately demarcate the region of interest before quantification of gut fluorescent intensity (using ImageJ software).

    Fluorescence:

    Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway
    Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the NIS elements software (Nikon), and emission at 340/380 nm was calculated. .. Cells were imaged during continuous perfusion with CIB and then challenged with sequential 30-s applications of 15 mM KCl, capsaicin (10 nM; Sigma-Aldrich), and 50 mM KCl, with 3-min intervening washouts with CIB.

    Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring.
    Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20.

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut
    Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon ECLIPSE Ti2 inverted microscope, NiS‐Elements D, version 5.30.02 software). .. Brightfield images of the gut were overlaid with fluorescent images to accurately demarcate the region of interest before quantification of gut fluorescent intensity (using ImageJ software).

    Automated:

    Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway
    Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the NIS elements software (Nikon), and emission at 340/380 nm was calculated. .. Cells were imaged during continuous perfusion with CIB and then challenged with sequential 30-s applications of 15 mM KCl, capsaicin (10 nM; Sigma-Aldrich), and 50 mM KCl, with 3-min intervening washouts with CIB.

    Imaging:

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Inverted Microscopy:

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut
    Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon ECLIPSE Ti2 inverted microscope, NiS‐Elements D, version 5.30.02 software). .. Brightfield images of the gut were overlaid with fluorescent images to accurately demarcate the region of interest before quantification of gut fluorescent intensity (using ImageJ software).

    Analysis:

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Light Microscopy:

    Article Title: Leptin promotes obesity‑related osteoarthritis progression via CD44/JAK2/STAT3 axis‑mediated chondrocyte apoptosis.
    Article Snippet: .. Following dehydration in increasing ethanol concentrations (70‐100%) and xylene clearing, the slides were mounted with neutral balsam and images were captured under a Nikon Eclipse 80i light microscope (Nikon Corporation,) using NIS‐Elements BR software (version 5.42; Nikon Corporation). ..

    Polymerase Chain Reaction:

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Amplification:

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Expressing:

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..



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    Image Search Results


    a , Clinical characteristics of the 15 samples used to build the atlases. Samples were collected from female and male donors with varying age and BMIs, as described in the pie charts. Characteristics were measured directly or extracted from donors’ medical records. b , The expression of the adipocyte marker adiponectin ( ADIPOQ ) in Uniform Manifold Approximation and Projection (UMAP) representations of hSAT and hVAT snRNA-seq atlases containing 37,879 and 83,731 nuclei, respectively. In both depots, adipocytes constitute a well-defined cluster. c , The expression of cell-specific markers in different clusters of each depot. Adipocytes were distinguished by multiple markers (marked in orange). d , The fraction of adipocytes per depot, stratified by sex, age and BMI in the snRNA-seq in-house data (hSAT, hVAT) and on estimating adipocyte proportions using deconvolution (Decon.) analysis of 73 paired bulk RNA-seq profiles (‘Decon’; hSAT:hVAT). Adipocytes were significantly more prevalent in hSAT versus hVAT in both datasets (two-sided Mann–Whitney U -test P = 0.0013 and P = 1.205 × 10 −13 , respectively), and tended to be more prevalent in females versus males according to the snRNA-seq data (two-sided Mann–Whitney U -test P = 0.044 in hVAT only). Other differences were not statistically significant. The boxplot central band indicates the median, the box limits the 25th to 75th percentiles and the whiskers 1.5× the interquartile range.

    Journal: Nature Genetics

    Article Title: Human subcutaneous and visceral adipocyte atlases uncover classical and nonclassical adipocytes and depot-specific patterns

    doi: 10.1038/s41588-024-02048-3

    Figure Lengend Snippet: a , Clinical characteristics of the 15 samples used to build the atlases. Samples were collected from female and male donors with varying age and BMIs, as described in the pie charts. Characteristics were measured directly or extracted from donors’ medical records. b , The expression of the adipocyte marker adiponectin ( ADIPOQ ) in Uniform Manifold Approximation and Projection (UMAP) representations of hSAT and hVAT snRNA-seq atlases containing 37,879 and 83,731 nuclei, respectively. In both depots, adipocytes constitute a well-defined cluster. c , The expression of cell-specific markers in different clusters of each depot. Adipocytes were distinguished by multiple markers (marked in orange). d , The fraction of adipocytes per depot, stratified by sex, age and BMI in the snRNA-seq in-house data (hSAT, hVAT) and on estimating adipocyte proportions using deconvolution (Decon.) analysis of 73 paired bulk RNA-seq profiles (‘Decon’; hSAT:hVAT). Adipocytes were significantly more prevalent in hSAT versus hVAT in both datasets (two-sided Mann–Whitney U -test P = 0.0013 and P = 1.205 × 10 −13 , respectively), and tended to be more prevalent in females versus males according to the snRNA-seq data (two-sided Mann–Whitney U -test P = 0.044 in hVAT only). Other differences were not statistically significant. The boxplot central band indicates the median, the box limits the 25th to 75th percentiles and the whiskers 1.5× the interquartile range.

    Article Snippet: Adipocytes were distinguished by multiple markers (marked in orange). d , The fraction of adipocytes per depot, stratified by sex, age and BMI in the snRNA-seq in-house data (hSAT, hVAT) and on estimating adipocyte proportions using deconvolution (Decon.) analysis of 73 paired bulk RNA-seq profiles (‘Decon’; hSAT:hVAT).

    Techniques: Expressing, Marker, RNA Sequencing, MANN-WHITNEY

    Percentages of classical and non-classical adipocytes in each depot separately were estimated in an independent set of samples analyzed by bulk RNA-seq (n = 73), using sNucConv deconvolution algorithm (a validated deconvolution tool for human adipose tissue based on paired samples sequenced by both bulk and snRNA-seq – see Methods for more detail). The percentage of classical adipocytes in hSAT was negatively correlated with waist-to-hip ratio (WHR) and HOMA-IR (Spearman r = -0.4 and -0.55, adjusted p = 0.17 and 0.06, respectively). The percentage of non-classical adipocytes in hSAT was positively correlated with WHR and HOMA-IR (Spearman r = 0.34 and 0.6, adjusted p = 0.17 and 0.05, respectively). The percentage of classical adipocytes in hVAT was negatively correlated with decease in triglycerides (TG) post bariatric surgery (BS) (Spearman r = 0.49, adjusted p = 0.1). Shaded grey areas mark a confidence interval of 0.95. P-values were adjusted using Benjamini-Hochberg procedure.

    Journal: Nature Genetics

    Article Title: Human subcutaneous and visceral adipocyte atlases uncover classical and nonclassical adipocytes and depot-specific patterns

    doi: 10.1038/s41588-024-02048-3

    Figure Lengend Snippet: Percentages of classical and non-classical adipocytes in each depot separately were estimated in an independent set of samples analyzed by bulk RNA-seq (n = 73), using sNucConv deconvolution algorithm (a validated deconvolution tool for human adipose tissue based on paired samples sequenced by both bulk and snRNA-seq – see Methods for more detail). The percentage of classical adipocytes in hSAT was negatively correlated with waist-to-hip ratio (WHR) and HOMA-IR (Spearman r = -0.4 and -0.55, adjusted p = 0.17 and 0.06, respectively). The percentage of non-classical adipocytes in hSAT was positively correlated with WHR and HOMA-IR (Spearman r = 0.34 and 0.6, adjusted p = 0.17 and 0.05, respectively). The percentage of classical adipocytes in hVAT was negatively correlated with decease in triglycerides (TG) post bariatric surgery (BS) (Spearman r = 0.49, adjusted p = 0.1). Shaded grey areas mark a confidence interval of 0.95. P-values were adjusted using Benjamini-Hochberg procedure.

    Article Snippet: Adipocytes were distinguished by multiple markers (marked in orange). d , The fraction of adipocytes per depot, stratified by sex, age and BMI in the snRNA-seq in-house data (hSAT, hVAT) and on estimating adipocyte proportions using deconvolution (Decon.) analysis of 73 paired bulk RNA-seq profiles (‘Decon’; hSAT:hVAT).

    Techniques: RNA Sequencing

    Triple co-localization of β-catenin, NDRG1, and PKCα demonstrates the formation of a possible metabolon, decreasing β-catenin levels and nuclear localization. A – H , NDRG1 overexpression in PANC-1 cells significantly increases the co-localization between β-catenin, NDRG1, and PKCα. A and B , VC and NDRG1 overexpressing PANC-1 cells were incubated for 24 h/37 °C in the presence and absence of WNT3a (100 ng/ml). The cells were then examined for β-catenin ( green ), NDRG1 ( red ), and PKCα ( blue ) expression and triple co-localization ( white ) using confocal immunofluorescence microscopy. Studies were performed using a 100× objective at the same acquisition setting with Olympus Fluoview FV3000 software. Images were digitally magnified for better demonstration of the possible co-localization. The scale bar = 3 μm for all images except for the inset images, where the scale bar = 9 μm. C and D , deconvolution analysis was then implemented on the images in ( A and B ) using Olympus CellSens imaging software. White arrows demonstrate triple co-localization and possible formation of the metabolon. The scale bar = 3 μm for all deconvolution images except for the inset images, where the scale bar = 1.8 μm. In all studies, the images are representative of three experiments, and the quantitative analysis of the pixel intensities is shown for ( E ) β-catenin; ( F ) NDRG1; ( G ) PKCα; and ( H ) Co-localization between β-catenin, NDRG1, and PKCα. Quantitative analyses were performed using ImageJ software and were presented as the mean ± SD ( n = 3). Analysis of pixel intensity and co-localization were performed using 24 cells. Statistical significance is denoted as ∗∗ p < 0.01 and ∗∗∗ p < 0.001 comparing NDRG1 overexpressing PANC-1 cells in the presence and absence of WNT3a to VC cells in the absence of WNT3a; or ### p < 0.001 comparing NDRG1 overexpressing PANC-1 cells to VC cells in the presence of WNT3a.

    Journal: The Journal of Biological Chemistry

    Article Title: Multi-modal mechanisms of the metastasis suppressor, NDRG1: Inhibition of WNT/β-catenin signaling by stabilization of protein kinase Cα

    doi: 10.1016/j.jbc.2024.107417

    Figure Lengend Snippet: Triple co-localization of β-catenin, NDRG1, and PKCα demonstrates the formation of a possible metabolon, decreasing β-catenin levels and nuclear localization. A – H , NDRG1 overexpression in PANC-1 cells significantly increases the co-localization between β-catenin, NDRG1, and PKCα. A and B , VC and NDRG1 overexpressing PANC-1 cells were incubated for 24 h/37 °C in the presence and absence of WNT3a (100 ng/ml). The cells were then examined for β-catenin ( green ), NDRG1 ( red ), and PKCα ( blue ) expression and triple co-localization ( white ) using confocal immunofluorescence microscopy. Studies were performed using a 100× objective at the same acquisition setting with Olympus Fluoview FV3000 software. Images were digitally magnified for better demonstration of the possible co-localization. The scale bar = 3 μm for all images except for the inset images, where the scale bar = 9 μm. C and D , deconvolution analysis was then implemented on the images in ( A and B ) using Olympus CellSens imaging software. White arrows demonstrate triple co-localization and possible formation of the metabolon. The scale bar = 3 μm for all deconvolution images except for the inset images, where the scale bar = 1.8 μm. In all studies, the images are representative of three experiments, and the quantitative analysis of the pixel intensities is shown for ( E ) β-catenin; ( F ) NDRG1; ( G ) PKCα; and ( H ) Co-localization between β-catenin, NDRG1, and PKCα. Quantitative analyses were performed using ImageJ software and were presented as the mean ± SD ( n = 3). Analysis of pixel intensity and co-localization were performed using 24 cells. Statistical significance is denoted as ∗∗ p < 0.01 and ∗∗∗ p < 0.001 comparing NDRG1 overexpressing PANC-1 cells in the presence and absence of WNT3a to VC cells in the absence of WNT3a; or ### p < 0.001 comparing NDRG1 overexpressing PANC-1 cells to VC cells in the presence of WNT3a.

    Article Snippet: The images of visualized cells were then examined using Olympus Fluoview software, and in some studies, images were processed for deconvolution analysis with CellSens software (Olympus) to improve contrast and image resolution.

    Techniques: Over Expression, Incubation, Expressing, Immunofluorescence, Microscopy, Software, Imaging