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il1α lentiviral expression vectors  (Addgene inc)


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    Addgene inc il1α lentiviral expression vectors
    Il1α Lentiviral Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcas9+expression+vector/pHAGE+EF1%CE%B1+dCas9-KRAB+(Plasmid+%2350919)/pm39117622-265-2-6
    Average 93 stars, based on 59 article reviews
    il1α lentiviral expression vectors - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Map functional insulators with cross-platform Hi-C meta-analysis
    Article Snippet: The dCas9 expression vectors used in this study were created using the Cas9 expression vector backbone from the pX330 plasmid (Addgene; plasmid 42230) through the In-Fusion cloning technique. .. The dCas9 genes were amplified from the pHAGE EF1α dCas9-KRAB plasmid (Addgene; plasmid 50919) via PCR and independently cloned into the AgeI and EcoRI sites of the pX330 plasmid, substituting the Cas9 ORF. ..

    Plasmid Preparation:

    Article Title: Map functional insulators with cross-platform Hi-C meta-analysis
    Article Snippet: The dCas9 expression vectors used in this study were created using the Cas9 expression vector backbone from the pX330 plasmid (Addgene; plasmid 42230) through the In-Fusion cloning technique. .. The dCas9 genes were amplified from the pHAGE EF1α dCas9-KRAB plasmid (Addgene; plasmid 50919) via PCR and independently cloned into the AgeI and EcoRI sites of the pX330 plasmid, substituting the Cas9 ORF. ..

    Article Title: Proximity labelling of pro-interleukin-1α reveals evolutionary conserved nuclear interactions.
    Article Snippet: Pro-IL-1α, pro-α-mat-β, orca pro-(h)IL-1α and pro-IL-1β genes were synthesised and cloned to pcDNA3.1(+) vectors (Life Technologies). .. To generate IL1α lentiviral expression vectors, Addgene plasmid #50919 was digested with BstBI and BstXI and a synthesised fragment comprising a multiple cloning site (MCS) and bGH polyA was inserted via restriction cloning, to create pLV-Ef1a-MCS. ..

    Article Title: Optimizing approaches for targeted integration of transgenic cassettes by integrase-mediated cassette exchange in mouse and human stem cells
    Article Snippet: For the CRISPRa exchange vector, the MS2-p65A-HSF1 cassette (Addgene #61423) was linked via a P2A cassette to dCas9-VP64-T2A-GFP (Addgene #61422) to create a single open reading frame expressing all components of the CRISPR-based SAM system and cloned between the Bxb1 attB sites. .. For the CRISPRi exchange vector, the dCas9-KRAB cassette (Addgene #50919) was subcloned between the Bxb1 attB sites. .. KOLF2-C1 (WTSIi018-B-1; RRID:CVCL_9S58 ) human iPS cells were cultured in Essential8 media (Thermofisher Scientific, #A1517001) on a Vitronectin substrate (Gibco, #A14700).

    Polymerase Chain Reaction:

    Article Title: Map functional insulators with cross-platform Hi-C meta-analysis
    Article Snippet: The dCas9 expression vectors used in this study were created using the Cas9 expression vector backbone from the pX330 plasmid (Addgene; plasmid 42230) through the In-Fusion cloning technique. .. The dCas9 genes were amplified from the pHAGE EF1α dCas9-KRAB plasmid (Addgene; plasmid 50919) via PCR and independently cloned into the AgeI and EcoRI sites of the pX330 plasmid, substituting the Cas9 ORF. ..

    Clone Assay:

    Article Title: Map functional insulators with cross-platform Hi-C meta-analysis
    Article Snippet: The dCas9 expression vectors used in this study were created using the Cas9 expression vector backbone from the pX330 plasmid (Addgene; plasmid 42230) through the In-Fusion cloning technique. .. The dCas9 genes were amplified from the pHAGE EF1α dCas9-KRAB plasmid (Addgene; plasmid 50919) via PCR and independently cloned into the AgeI and EcoRI sites of the pX330 plasmid, substituting the Cas9 ORF. ..

    Expressing:

    Article Title: Fitness Screens Map State-Specific Glioblastoma Stem Cell Vulnerabilities
    Article Snippet: 2 Glioblastoma (GBM) is the most common and lethal primary brain tumor in adults and is driven 33 by self-renewing glioblastoma stem cells (GSCs) that persist after therapy and seed treatment 34 refractory recurrent tumors.. GBM tumors display a high degree of intra- and inter-tumoral 35 heterogeneity that is a prominent barrier to targeted treatment strategies.. This heterogeneity 36 extends to GSCs that exist on a gradient between two transcriptional states or subtypes termed 37 developmental and injury-response.

    Article Title: Proximity labelling of pro-interleukin-1α reveals evolutionary conserved nuclear interactions.
    Article Snippet: Pro-IL-1α, pro-α-mat-β, orca pro-(h)IL-1α and pro-IL-1β genes were synthesised and cloned to pcDNA3.1(+) vectors (Life Technologies). .. To generate IL1α lentiviral expression vectors, Addgene plasmid #50919 was digested with BstBI and BstXI and a synthesised fragment comprising a multiple cloning site (MCS) and bGH polyA was inserted via restriction cloning, to create pLV-Ef1a-MCS. ..

    Cloning:

    Article Title: Proximity labelling of pro-interleukin-1α reveals evolutionary conserved nuclear interactions.
    Article Snippet: Pro-IL-1α, pro-α-mat-β, orca pro-(h)IL-1α and pro-IL-1β genes were synthesised and cloned to pcDNA3.1(+) vectors (Life Technologies). .. To generate IL1α lentiviral expression vectors, Addgene plasmid #50919 was digested with BstBI and BstXI and a synthesised fragment comprising a multiple cloning site (MCS) and bGH polyA was inserted via restriction cloning, to create pLV-Ef1a-MCS. ..

    Transfection:

    Article Title: Accessibility at a primed distal Fshb-Kcna4 super-enhancer is facilitated by Foxl2 during gonadotrope differentiation.
    Article Snippet: As noted, they were treated with vehicle alone (non-treated controls), 13 Activin A (5 nM; #SRP6057, Sigma or Peprotech #120-14P, Gibco) or JQ-1 (0.25 or 1 μM; 14 #CAY-11187-1, Cayman Chemicals). .. CRISPRi was carried out as previously22 following stable 15 transfections of dCas9-KRAB (Addgene #50919) and gRNA oligos (Table S128). ..



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    Figure 1. Effects of different sequences of sgRNAs (sgRNA1-3), including the vector and positive control sgRNA, on gene expression according to RT‒qPCR at 0 and 24 h. (a-e) Fold changes in the <t>dCas9</t> gene. (f-g) Fold changes in the number of cells positive for the mmpL3 gene. (h-k) Fold changes in the inhA gene. (l) Comparison of the fold changes in inhA gene expression after 24 h of aTc activation. Statistical significance was determined using nonparametric tests (Mann‒Whitney U test) for comparisons between inactivated and activated samples using 100 ng/ml of aTc at each time point for different strains (a-k). The Kruskal‒Wallis test was performed to analyze the significant differences in inhA gene expression from different sgRNAs after induction by aTc for 24 h (l). Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).
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    Image Search Results


    Figure 1. Effects of different sequences of sgRNAs (sgRNA1-3), including the vector and positive control sgRNA, on gene expression according to RT‒qPCR at 0 and 24 h. (a-e) Fold changes in the dCas9 gene. (f-g) Fold changes in the number of cells positive for the mmpL3 gene. (h-k) Fold changes in the inhA gene. (l) Comparison of the fold changes in inhA gene expression after 24 h of aTc activation. Statistical significance was determined using nonparametric tests (Mann‒Whitney U test) for comparisons between inactivated and activated samples using 100 ng/ml of aTc at each time point for different strains (a-k). The Kruskal‒Wallis test was performed to analyze the significant differences in inhA gene expression from different sgRNAs after induction by aTc for 24 h (l). Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).

    Journal: Scientific reports

    Article Title: The CRISPR-dCas9 interference system suppresses inhA gene expression in Mycobacterium smegmatis.

    doi: 10.1038/s41598-024-77442-2

    Figure Lengend Snippet: Figure 1. Effects of different sequences of sgRNAs (sgRNA1-3), including the vector and positive control sgRNA, on gene expression according to RT‒qPCR at 0 and 24 h. (a-e) Fold changes in the dCas9 gene. (f-g) Fold changes in the number of cells positive for the mmpL3 gene. (h-k) Fold changes in the inhA gene. (l) Comparison of the fold changes in inhA gene expression after 24 h of aTc activation. Statistical significance was determined using nonparametric tests (Mann‒Whitney U test) for comparisons between inactivated and activated samples using 100 ng/ml of aTc at each time point for different strains (a-k). The Kruskal‒Wallis test was performed to analyze the significant differences in inhA gene expression from different sgRNAs after induction by aTc for 24 h (l). Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).

    Article Snippet: The PLJR962 CRISPRi vector expressing Streptococcus thermophilus dCas9 (SthdCas9) with a specific site for inserted sgRNAs (Addgene plasmid #115162) was kindly provided by Dr. Sarah Fortune (Department of Immunology and Infectious Diseases, Harvard T.H.

    Techniques: Plasmid Preparation, Positive Control, Gene Expression, Comparison, Activation Assay, Standard Deviation

    Figure 3. Effects of different aTc concentrations for CRISPRi activation on gene expression, as determined by RT‒qPCR at 0 and 24 h. (a-c) Fold changes in the dCas9 gene. (d-e) Fold changes in the number of positive sgRNAs and mmpL3 gene expression. (f-g) Fold changes in the inhA gene. Statistical significance was determined using nonparametric ANOVA (Kruskal‒Wallis test) to compare Msm inactivated and activated by 50, 100 or 200 ng/ml of aTc at each time point with the vector control, positive sgRNA, or sgRNA2. Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).

    Journal: Scientific reports

    Article Title: The CRISPR-dCas9 interference system suppresses inhA gene expression in Mycobacterium smegmatis.

    doi: 10.1038/s41598-024-77442-2

    Figure Lengend Snippet: Figure 3. Effects of different aTc concentrations for CRISPRi activation on gene expression, as determined by RT‒qPCR at 0 and 24 h. (a-c) Fold changes in the dCas9 gene. (d-e) Fold changes in the number of positive sgRNAs and mmpL3 gene expression. (f-g) Fold changes in the inhA gene. Statistical significance was determined using nonparametric ANOVA (Kruskal‒Wallis test) to compare Msm inactivated and activated by 50, 100 or 200 ng/ml of aTc at each time point with the vector control, positive sgRNA, or sgRNA2. Error bars represent the standard deviation from the mean of triplicate experiments (*p < 0.05, **p ≤ 0.01, ***p ≤ 0.001).

    Article Snippet: The PLJR962 CRISPRi vector expressing Streptococcus thermophilus dCas9 (SthdCas9) with a specific site for inserted sgRNAs (Addgene plasmid #115162) was kindly provided by Dr. Sarah Fortune (Department of Immunology and Infectious Diseases, Harvard T.H.

    Techniques: Activation Assay, Gene Expression, Plasmid Preparation, Control, Standard Deviation