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dba2  (Jackson Laboratory)

 
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    Jackson Laboratory dba2
    Figure 4. Efficacy of reconstitution of midi-dystrophins in <t>Dba2-mdx</t> mouse model. (A) Schematic representation of the in vivo protocol. One month old Dba2/mdx mice were injected with midi-Dys 1, midi-Dys 2, and midi-Dys 3 at a dose of 2.0 × 1013 vg/kg each vector (total dose 4.0 × 1013 vg/kg).
    Dba2, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "In Silico Structural Prediction for the Generation of Novel Performant Midi-Dystrophins Based on Intein-Mediated Dual AAV Approach."

    Article Title: In Silico Structural Prediction for the Generation of Novel Performant Midi-Dystrophins Based on Intein-Mediated Dual AAV Approach.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms251910444

    Figure 4. Efficacy of reconstitution of midi-dystrophins in Dba2-mdx mouse model. (A) Schematic representation of the in vivo protocol. One month old Dba2/mdx mice were injected with midi-Dys 1, midi-Dys 2, and midi-Dys 3 at a dose of 2.0 × 1013 vg/kg each vector (total dose 4.0 × 1013 vg/kg).
    Figure Legend Snippet: Figure 4. Efficacy of reconstitution of midi-dystrophins in Dba2-mdx mouse model. (A) Schematic representation of the in vivo protocol. One month old Dba2/mdx mice were injected with midi-Dys 1, midi-Dys 2, and midi-Dys 3 at a dose of 2.0 × 1013 vg/kg each vector (total dose 4.0 × 1013 vg/kg).

    Techniques Used: In Vivo, Injection, Plasmid Preparation

    Figure 5. Functional and histopathological therapeutic efficacy in Dba2-mdx mice. (A) CPK (creatine phosphokinase) concentration in the sera of Dba2-mdx mice injected with midi-Dys 1-2-3. Results are
    Figure Legend Snippet: Figure 5. Functional and histopathological therapeutic efficacy in Dba2-mdx mice. (A) CPK (creatine phosphokinase) concentration in the sera of Dba2-mdx mice injected with midi-Dys 1-2-3. Results are

    Techniques Used: Functional Assay, Drug discovery, Concentration Assay, Injection

    Related Articles

    In Vivo:

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Article Title: Specific Innate Immune Cells Uptake Fetal Antigen and Display Homeostatic Phenotypes in the Maternal Circulation
    Article Snippet: C57BL/6-Tg(CAG-OVAL)916Jen/J (Act-mOVA II) (hereafter referred to as B6 CAG-OVA) male, BALB/cByJ (BALB/c) female and male, C57BL/6 female and C57BL/6 male (hereafter referred to as B6 non-CAG-OVA), C57BL/6 Actb-Egfp (GFP+) male, and DBA/2 female mice were purchased from The Jackson Laboratory (Bar Harbor, ME), bred in the animal care facility at the C.S.

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Injection:

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Article Title: Specific Innate Immune Cells Uptake Fetal Antigen and Display Homeostatic Phenotypes in the Maternal Circulation
    Article Snippet: C57BL/6-Tg(CAG-OVAL)916Jen/J (Act-mOVA II) (hereafter referred to as B6 CAG-OVA) male, BALB/cByJ (BALB/c) female and male, C57BL/6 female and C57BL/6 male (hereafter referred to as B6 non-CAG-OVA), C57BL/6 Actb-Egfp (GFP+) male, and DBA/2 female mice were purchased from The Jackson Laboratory (Bar Harbor, ME), bred in the animal care facility at the C.S.

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Plasmid Preparation:

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Article Title: Specific Innate Immune Cells Uptake Fetal Antigen and Display Homeostatic Phenotypes in the Maternal Circulation
    Article Snippet: C57BL/6-Tg(CAG-OVAL)916Jen/J (Act-mOVA II) (hereafter referred to as B6 CAG-OVA) male, BALB/cByJ (BALB/c) female and male, C57BL/6 female and C57BL/6 male (hereafter referred to as B6 non-CAG-OVA), C57BL/6 Actb-Egfp (GFP+) male, and DBA/2 female mice were purchased from The Jackson Laboratory (Bar Harbor, ME), bred in the animal care facility at the C.S.

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Functional Assay:

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Article Title: Specific Innate Immune Cells Uptake Fetal Antigen and Display Homeostatic Phenotypes in the Maternal Circulation
    Article Snippet: C57BL/6-Tg(CAG-OVAL)916Jen/J (Act-mOVA II) (hereafter referred to as B6 CAG-OVA) male, BALB/cByJ (BALB/c) female and male, C57BL/6 female and C57BL/6 male (hereafter referred to as B6 non-CAG-OVA), C57BL/6 Actb-Egfp (GFP+) male, and DBA/2 female mice were purchased from The Jackson Laboratory (Bar Harbor, ME), bred in the animal care facility at the C.S.

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Drug discovery:

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Article Title: Specific Innate Immune Cells Uptake Fetal Antigen and Display Homeostatic Phenotypes in the Maternal Circulation
    Article Snippet: C57BL/6-Tg(CAG-OVAL)916Jen/J (Act-mOVA II) (hereafter referred to as B6 CAG-OVA) male, BALB/cByJ (BALB/c) female and male, C57BL/6 female and C57BL/6 male (hereafter referred to as B6 non-CAG-OVA), C57BL/6 Actb-Egfp (GFP+) male, and DBA/2 female mice were purchased from The Jackson Laboratory (Bar Harbor, ME), bred in the animal care facility at the C.S.

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Concentration Assay:

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.

    Article Title: Specific Innate Immune Cells Uptake Fetal Antigen and Display Homeostatic Phenotypes in the Maternal Circulation
    Article Snippet: C57BL/6-Tg(CAG-OVAL)916Jen/J (Act-mOVA II) (hereafter referred to as B6 CAG-OVA) male, BALB/cByJ (BALB/c) female and male, C57BL/6 female and C57BL/6 male (hereafter referred to as B6 non-CAG-OVA), C57BL/6 Actb-Egfp (GFP+) male, and DBA/2 female mice were purchased from The Jackson Laboratory (Bar Harbor, ME), bred in the animal care facility at the C.S.

    Article Title: Adjuvants and MHCII modulate the immunogenicity of subdominant epitopes in Plasmodium vivax Duffy binding protein
    Article Snippet: Male and/or female BALB/c (stock no.:028) and female C57BL/6 (stock no.:027) mice were purchased from Charles River, Canada and B10.D2 (stock no.:000463), B10 (stock no.:000666), and DBA/2 (stock no.:000671) female mice were purchased from The Jackson Laboratory, USA.



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    Image Search Results


    Combined therapy: Gene transfer efficiency (A) Schematic representation of the in vivo protocol. Two-month-old D2.Mdx mice were injected or not with two consecutive doses (1 × 10 6 ) of control T cells or of FAP-CAR-T cells, and after 2 weeks of treatment the mice were injected with a single intravenous dose (5 × 10 12 VG/kg) of rAAV9-microdystrophin (μDystrophin or μDys). Tissues were collected 6 weeks after the AAV injection, 4 animals were included per group and statistical analyses used one-way ANOVA tests with p values indicated above the compared conditions. (B) Quantification of VGCN with ddPCR in TA, EDL, and DIA in DBA2-MDX mice. (C) Gene expression analysis of microdystrophin in TA, EDL, and DIA of DBA2-MDX mice. Results are shown as a histogram of relative abundance of gene expression over P0 normalizer gene. (D) Quantification of microdystrophin expression after capillary western blot with DYS-B antibody in TA, EDL, and DIA at 6 weeks post-injection in mice. Values are represented as normalized area under the curve (AUC). (E) Capillary western blot of protein lysates of TA at 6 weeks post-injection in mice. Microdystrophin are visible around 139 kDa. (F) Representative microdystrophin IHF pictures of the TA, EDL, and DIA at 6 weeks post-injection in mice. Images include a scale bar. (G) Quantification of dystrophin positive myofibers in TA, EDL, and DIA at 6 weeks post-injection in D2.mdx mice. The percentage of dystrophin+ fibers is represented of number of dystrophin positive fibers over fibers positive for laminin.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: FAP-CAR-T cells reduce dystrophic muscle fibrosis, improving adeno-associated virus gene transfer efficacy

    doi: 10.1016/j.omtm.2025.101545

    Figure Lengend Snippet: Combined therapy: Gene transfer efficiency (A) Schematic representation of the in vivo protocol. Two-month-old D2.Mdx mice were injected or not with two consecutive doses (1 × 10 6 ) of control T cells or of FAP-CAR-T cells, and after 2 weeks of treatment the mice were injected with a single intravenous dose (5 × 10 12 VG/kg) of rAAV9-microdystrophin (μDystrophin or μDys). Tissues were collected 6 weeks after the AAV injection, 4 animals were included per group and statistical analyses used one-way ANOVA tests with p values indicated above the compared conditions. (B) Quantification of VGCN with ddPCR in TA, EDL, and DIA in DBA2-MDX mice. (C) Gene expression analysis of microdystrophin in TA, EDL, and DIA of DBA2-MDX mice. Results are shown as a histogram of relative abundance of gene expression over P0 normalizer gene. (D) Quantification of microdystrophin expression after capillary western blot with DYS-B antibody in TA, EDL, and DIA at 6 weeks post-injection in mice. Values are represented as normalized area under the curve (AUC). (E) Capillary western blot of protein lysates of TA at 6 weeks post-injection in mice. Microdystrophin are visible around 139 kDa. (F) Representative microdystrophin IHF pictures of the TA, EDL, and DIA at 6 weeks post-injection in mice. Images include a scale bar. (G) Quantification of dystrophin positive myofibers in TA, EDL, and DIA at 6 weeks post-injection in D2.mdx mice. The percentage of dystrophin+ fibers is represented of number of dystrophin positive fibers over fibers positive for laminin.

    Article Snippet: The DBA2 (B6; 129S4-DBA2tm1Cpr/J, strain no. 000671) (D2) controls mice were purchased directly from Charles River Laboratories) while the DBA2/mdx (D2.B10-Dmdmdx/J, strain no. 013141) (D2.mdx) mice were bred and obtained from the Center d’Exploration et de Recherche Fonctionnelle Expérimentale (CERFE) in Evry, France.

    Techniques: In Vivo, Injection, Control, Gene Expression, Expressing, Western Blot

    Combined therapy: Histology effects Mice were treated as in A. (A) Representative images with scale bars, of hematoxylin and eosine, Sirius red, alizarine red coloration, and CD11b staining in the TA, EDL, and diaphragm of Dba2-mdx mice 6 weeks post-injection. For Sirius red coloration, red-colored regions are highly fibrotic and contain collagen deposits. For alizarine coloration, brown areas are indicated as a region containing calcium deposits. For CD11b staining, red signals indicate monocytes cells. (B) Quantification of the collagen, calcium deposits area and CD11b cells in the TA of mice. The results are shown as the percentage of fibrotic positive or calcium positive regions over the total area of the muscle cut for colorations. The results for CD11b staining are shown as the percentage of CD11b + cells after nuclei segmentation on R software. Statistical p values are indicated above the compared conditions.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: FAP-CAR-T cells reduce dystrophic muscle fibrosis, improving adeno-associated virus gene transfer efficacy

    doi: 10.1016/j.omtm.2025.101545

    Figure Lengend Snippet: Combined therapy: Histology effects Mice were treated as in A. (A) Representative images with scale bars, of hematoxylin and eosine, Sirius red, alizarine red coloration, and CD11b staining in the TA, EDL, and diaphragm of Dba2-mdx mice 6 weeks post-injection. For Sirius red coloration, red-colored regions are highly fibrotic and contain collagen deposits. For alizarine coloration, brown areas are indicated as a region containing calcium deposits. For CD11b staining, red signals indicate monocytes cells. (B) Quantification of the collagen, calcium deposits area and CD11b cells in the TA of mice. The results are shown as the percentage of fibrotic positive or calcium positive regions over the total area of the muscle cut for colorations. The results for CD11b staining are shown as the percentage of CD11b + cells after nuclei segmentation on R software. Statistical p values are indicated above the compared conditions.

    Article Snippet: The DBA2 (B6; 129S4-DBA2tm1Cpr/J, strain no. 000671) (D2) controls mice were purchased directly from Charles River Laboratories) while the DBA2/mdx (D2.B10-Dmdmdx/J, strain no. 013141) (D2.mdx) mice were bred and obtained from the Center d’Exploration et de Recherche Fonctionnelle Expérimentale (CERFE) in Evry, France.

    Techniques: Staining, Injection, Software

    Figure 4. Efficacy of reconstitution of midi-dystrophins in Dba2-mdx mouse model. (A) Schematic representation of the in vivo protocol. One month old Dba2/mdx mice were injected with midi-Dys 1, midi-Dys 2, and midi-Dys 3 at a dose of 2.0 × 1013 vg/kg each vector (total dose 4.0 × 1013 vg/kg).

    Journal: International journal of molecular sciences

    Article Title: In Silico Structural Prediction for the Generation of Novel Performant Midi-Dystrophins Based on Intein-Mediated Dual AAV Approach.

    doi: 10.3390/ijms251910444

    Figure Lengend Snippet: Figure 4. Efficacy of reconstitution of midi-dystrophins in Dba2-mdx mouse model. (A) Schematic representation of the in vivo protocol. One month old Dba2/mdx mice were injected with midi-Dys 1, midi-Dys 2, and midi-Dys 3 at a dose of 2.0 × 1013 vg/kg each vector (total dose 4.0 × 1013 vg/kg).

    Article Snippet: DBA2 (B6;129S4-DBA2tm1Cpr/J, strain #000671) and DBA2-mdx (D2.B10-Dmdmdx/J) mice were supplied by the Jackson Laboratory.

    Techniques: In Vivo, Injection, Plasmid Preparation

    Figure 5. Functional and histopathological therapeutic efficacy in Dba2-mdx mice. (A) CPK (creatine phosphokinase) concentration in the sera of Dba2-mdx mice injected with midi-Dys 1-2-3. Results are

    Journal: International journal of molecular sciences

    Article Title: In Silico Structural Prediction for the Generation of Novel Performant Midi-Dystrophins Based on Intein-Mediated Dual AAV Approach.

    doi: 10.3390/ijms251910444

    Figure Lengend Snippet: Figure 5. Functional and histopathological therapeutic efficacy in Dba2-mdx mice. (A) CPK (creatine phosphokinase) concentration in the sera of Dba2-mdx mice injected with midi-Dys 1-2-3. Results are

    Article Snippet: DBA2 (B6;129S4-DBA2tm1Cpr/J, strain #000671) and DBA2-mdx (D2.B10-Dmdmdx/J) mice were supplied by the Jackson Laboratory.

    Techniques: Functional Assay, Drug discovery, Concentration Assay, Injection