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dna microarray dataset  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna microarray dataset
    Dna Microarray Dataset, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/datasets+of+dna+microarray/DNA/pm41662993-77-0-3
    Average 99 stars, based on 1 article reviews
    dna microarray dataset - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: A national study confirms a low occurrence of antimicrobial resistance amongst Escherichia coli isolates from the caecae of Australian meat chickens
    Article Snippet: .. DNA was extracted using the MagMAXTM Multi-Sample DNA Extraction Kit (Thermo Fisher Scientific, USA), following the manufacturer’s instructions. .. DNA libraries were prepared using the Celero chemistry (Tecan) with a modified reaction volume deviating from the manufacturer’s standard protocol.

    Concentration Assay:

    Article Title: Development of a high-resolution multiplex qPCR method to profile microbial consortia in spaceflight water recovery systems
    Article Snippet: 3 mL of broth culture from each strain was harvested by centrifugation and the genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen), following the manufacturer's protocol for cultured cells. .. DNA concentration and purity were assessed using a NanoDrop spectrophotometer. ..

    Article Title: Integrated genomic, transcriptomic, and metabolomic profile analysis reveals molecular mechanism underlying meat quality traits in Chinese Kangle chickens
    Article Snippet: .. We extracted genomic DNA from blood samples using the phenol/chloroform method and quantified DNA concentration/quality using a Nanodrop-2000 spectrophotometer (Thermo Fisher Scientific, MA, USA). .. In total, 273 Chinese Kangle chickens were genotyped for 52,180 SNPs using the “Jingxin NO.1” 55 K Beadchip by following the manufacturer's protocol (Chinese Academy of Agricultural Sciences, Beijing, China) ( ).

    Spectrophotometry:

    Article Title: Development of a high-resolution multiplex qPCR method to profile microbial consortia in spaceflight water recovery systems
    Article Snippet: 3 mL of broth culture from each strain was harvested by centrifugation and the genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen), following the manufacturer's protocol for cultured cells. .. DNA concentration and purity were assessed using a NanoDrop spectrophotometer. ..

    Article Title: Integrated genomic, transcriptomic, and metabolomic profile analysis reveals molecular mechanism underlying meat quality traits in Chinese Kangle chickens
    Article Snippet: .. We extracted genomic DNA from blood samples using the phenol/chloroform method and quantified DNA concentration/quality using a Nanodrop-2000 spectrophotometer (Thermo Fisher Scientific, MA, USA). .. In total, 273 Chinese Kangle chickens were genotyped for 52,180 SNPs using the “Jingxin NO.1” 55 K Beadchip by following the manufacturer's protocol (Chinese Academy of Agricultural Sciences, Beijing, China) ( ).

    Polymerase Chain Reaction:

    Article Title: Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction in Yarrowia lipolytica
    Article Snippet: The codon-optimized exogenous AaFS gene and its mutant variants synthesized by GenScript (Nanjing, China) were tailored for Y. lipolytica expression. .. PCR amplification was performed using primers listed in (Phusion® High-Fidelity DNA Polymerase, Thermo Fisher Scientific). ..

    Amplification:

    Article Title: Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction in Yarrowia lipolytica
    Article Snippet: The codon-optimized exogenous AaFS gene and its mutant variants synthesized by GenScript (Nanjing, China) were tailored for Y. lipolytica expression. .. PCR amplification was performed using primers listed in (Phusion® High-Fidelity DNA Polymerase, Thermo Fisher Scientific). ..

    Viscosity:

    Article Title: Protocol for the assessment of the impact of mycotoxins and glyphosate residues on the gut microbiome and resistome of European fallow deer
    Article Snippet: .. Qubit quantification indicated acceptable DNA concentrations; however, NanoDrop measurements showed low 260/230 ratios (e.g., 0.3–1.2), and the DNA exhibited a brown coloration and increased viscosity. ..

    other:

    Article Title: Integrative genomic analysis and gene expression patterns reveal a cardio-neuroendocrine signaling network for heat adaptation in geographically diverse chickens
    Article Snippet: DNA extraction was performed, checked for DNA quality using 1% agarose gel electrophoresis and NanoDrop 2000 (A260/280 ratio), and finally libraries were sequenced on the DNBSEQ-T7 platform.

    Modification:

    Article Title: Amino acid starvation and iron limitation facilitate the biofilm formation of Klebsiella pneumoniae within urine
    Article Snippet: .. All enzymes required for restriction digestion and DNA modification were sourced from Thermo Fisher Scientific (Waltham, MA, USA) and handled in accordance with the supplier's protocols. ..

    Purification:

    Article Title: Identification of biofilm-associated genes in Arcobacter butzleri
    Article Snippet: For that, the DNA of the P8 strain was extracted using Lysing Matrix E tubes (MP Biomedicals, France) and a Bead Mill 4 Mini Homogenizer (Fisher Scientific, USA). .. After purification with AMPure XP beads (Beckman Coulter, USA), DNA quantification was performed by a Qubit fluorometer (Invitrogen, USA) using the Qubit 1X dsDNA HS assay kit (Invitrogen, USA). .. DNA libraries were then prepared using the Rapid Sequencing Kit V14 (Oxford Nanopore Technologies, UK) and loaded into a Flonge flow cell (Oxford Nanopore Technologies, UK) for sequencing.



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    Image Search Results


    Low-dimensional representation of variable DNA methylation sites across all placental and immune cell types and whole placental tissue samples shows samples cluster by cell type.

    Journal: Epigenetics

    Article Title: Placental and immune cell DNA methylation reference panel for bulk tissue cell composition estimation in epidemiological studies

    doi: 10.1080/15592294.2024.2437275

    Figure Lengend Snippet: Low-dimensional representation of variable DNA methylation sites across all placental and immune cell types and whole placental tissue samples shows samples cluster by cell type.

    Article Snippet: To assess the performance of our placental DNA methylation deconvolution reference, we compared its ability to predict DNA methylation variation [ ] in a preprocessed placental whole tissue Illumina EPIC microarray DNA methylation dataset comprising 204 placentas across three cohorts (GEO accession number GSE232778) [ ] against the only existing multi-cell type reference [ ].

    Techniques: DNA Methylation Assay

    Analytic table of  DNA   methylation  samples used for deconvolution reference.

    Journal: Epigenetics

    Article Title: Placental and immune cell DNA methylation reference panel for bulk tissue cell composition estimation in epidemiological studies

    doi: 10.1080/15592294.2024.2437275

    Figure Lengend Snippet: Analytic table of DNA methylation samples used for deconvolution reference.

    Article Snippet: To assess the performance of our placental DNA methylation deconvolution reference, we compared its ability to predict DNA methylation variation [ ] in a preprocessed placental whole tissue Illumina EPIC microarray DNA methylation dataset comprising 204 placentas across three cohorts (GEO accession number GSE232778) [ ] against the only existing multi-cell type reference [ ].

    Techniques: DNA Methylation Assay, Fluorescence

    Placental cell type differential DNA methylation. Volcano plots comparing site-specific DNA methylation levels in one cell type against the average across all other cell types.

    Journal: Epigenetics

    Article Title: Placental and immune cell DNA methylation reference panel for bulk tissue cell composition estimation in epidemiological studies

    doi: 10.1080/15592294.2024.2437275

    Figure Lengend Snippet: Placental cell type differential DNA methylation. Volcano plots comparing site-specific DNA methylation levels in one cell type against the average across all other cell types.

    Article Snippet: To assess the performance of our placental DNA methylation deconvolution reference, we compared its ability to predict DNA methylation variation [ ] in a preprocessed placental whole tissue Illumina EPIC microarray DNA methylation dataset comprising 204 placentas across three cohorts (GEO accession number GSE232778) [ ] against the only existing multi-cell type reference [ ].

    Techniques: DNA Methylation Assay

    Top differentially methylated sites unique to the five placental cell types (cytotrophoblast, endothelial, Hofbauer, stromal, and syncytiotrophoblast) and nucleated red blood cells with gene annotations reveal highly specific DNA methylation profiles by cell type.

    Journal: Epigenetics

    Article Title: Placental and immune cell DNA methylation reference panel for bulk tissue cell composition estimation in epidemiological studies

    doi: 10.1080/15592294.2024.2437275

    Figure Lengend Snippet: Top differentially methylated sites unique to the five placental cell types (cytotrophoblast, endothelial, Hofbauer, stromal, and syncytiotrophoblast) and nucleated red blood cells with gene annotations reveal highly specific DNA methylation profiles by cell type.

    Article Snippet: To assess the performance of our placental DNA methylation deconvolution reference, we compared its ability to predict DNA methylation variation [ ] in a preprocessed placental whole tissue Illumina EPIC microarray DNA methylation dataset comprising 204 placentas across three cohorts (GEO accession number GSE232778) [ ] against the only existing multi-cell type reference [ ].

    Techniques: Methylation, DNA Methylation Assay

    Summary graphs of the root mean square error of prediction in the ability to model principal components of DNA methylation in 204 placenta dataset GSE232778 for our expanded reference versus the existing reference implemented in planet from 1,000 repeats of a 10-fold cross-validation linear regression model with estimated cell composition as the predictor variables.

    Journal: Epigenetics

    Article Title: Placental and immune cell DNA methylation reference panel for bulk tissue cell composition estimation in epidemiological studies

    doi: 10.1080/15592294.2024.2437275

    Figure Lengend Snippet: Summary graphs of the root mean square error of prediction in the ability to model principal components of DNA methylation in 204 placenta dataset GSE232778 for our expanded reference versus the existing reference implemented in planet from 1,000 repeats of a 10-fold cross-validation linear regression model with estimated cell composition as the predictor variables.

    Article Snippet: To assess the performance of our placental DNA methylation deconvolution reference, we compared its ability to predict DNA methylation variation [ ] in a preprocessed placental whole tissue Illumina EPIC microarray DNA methylation dataset comprising 204 placentas across three cohorts (GEO accession number GSE232778) [ ] against the only existing multi-cell type reference [ ].

    Techniques: DNA Methylation Assay, Biomarker Discovery