miscript mirna pcr array data analysis excel template (Qiagen)
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Miscript Mirna Pcr Array Data Analysis Excel Template, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data-analysis-template/miscript+ii+rt+kit/pm37655273-52-6-20
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Generated:Article Title: miR-769-5p has diagnostic value in acute kidney injury in intensive care unit patients and mediates disease development by targeting SIRT6. Article Snippet: .. For miRNAs such as miR-769-5p, cDNA was generated with the use of other:Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines Article Snippet: Article Title: Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma Article Snippet: The expression level of miR-30a-5p was assessed using miR-30a-5p and Synthesized:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using Quantitative RT-PCR:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using SYBR Green Assay:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines Article Snippet: The miScript II RT kit (cat. no. 218161; Qiagen, Inc.) was used for the reverse transcription of RNA into cDNA, adhering to the manufacturer's protocol for miRs. .. To detect miR-145 and miR-23b levels, the Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines Article Snippet: Small nuclear RNA U6 (forward primer, 5′-CGCTTCGGCAGCACATATACTA-3′; cat. no. MS00033740; Qiagen, Inc.) was used as a miR housekeeping control. .. The reverse primer is included in the Polymerase Chain Reaction:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using Sequencing:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using Real-time Polymerase Chain Reaction:Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines Article Snippet: The miScript II RT kit (cat. no. 218161; Qiagen, Inc.) was used for the reverse transcription of RNA into cDNA, adhering to the manufacturer's protocol for miRs. .. To detect miR-145 and miR-23b levels, the Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines Article Snippet: Small nuclear RNA U6 (forward primer, 5′-CGCTTCGGCAGCACATATACTA-3′; cat. no. MS00033740; Qiagen, Inc.) was used as a miR housekeeping control. .. The reverse primer is included in the Reverse Transcription:Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines Article Snippet: Extraction of miRs was carried out using chemical and mechanical lysis using QIAzol lysis reagent (Qiagen, Inc.), followed by the miRNeasy kit (Qiagen, Inc.), according to the manufacturer's instructions. .. The cDNA Synthesis:Article Title: Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma Article Snippet: .. Complementary DNA (cDNA) synthesis was carried out with 1 μg of miRNA using the |
