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miscript mirna pcr array data analysis excel template  (Qiagen)


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    Qiagen miscript mirna pcr array data analysis excel template
    Miscript Mirna Pcr Array Data Analysis Excel Template, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data-analysis-template/miscript+ii+rt+kit/pm37655273-52-6-20
    Average 90 stars, based on 1 article reviews
    miscript mirna pcr array data analysis excel template - by Bioz Stars, 2026-09
    90/100 stars

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    Generated:

    Article Title: miR-769-5p has diagnostic value in acute kidney injury in intensive care unit patients and mediates disease development by targeting SIRT6.
    Article Snippet: .. For miRNAs such as miR-769-5p, cDNA was generated with the use of miScript II RT Kit (Qiagen, Germany). .. Reverse transcription was performed for mRNA of SIRT6, renal tubular injury markers kidney injury molecule-1 (KIM-1), and Neutrophil gelatinase-associated lipocalin (NGAL) using SuperScriptTM IV Reverse Transcriptase Kit (Invitrogen, USA). miR-769-5p were amplified using miScript SYBR Green PCR Kit (Qiagen, Germany).

    other:

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: Small nuclear RNA U6 (forward primer, 5′-CGCTTCGGCAGCACATATACTA-3′; cat. no. MS00033740; Qiagen, Inc.) was used as a miR housekeeping control.

    Article Title: Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma
    Article Snippet: The expression level of miR-30a-5p was assessed using miR-30a-5p and U6 small RNA (as the endogenous control) miScript primer assays (Qiagen GmbH, Hilden, Germany). miR-30a-5p expression was determined using the miScript SYBR® Green PCR Kit (Qiagen GmbH, Hilden, Germany), following the manufacturer's protocol.

    Synthesized:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    SYBR Green Assay:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: The miScript II RT kit (cat. no. 218161; Qiagen, Inc.) was used for the reverse transcription of RNA into cDNA, adhering to the manufacturer's protocol for miRs. .. To detect miR-145 and miR-23b levels, the miScript SYBR Green qPCR Kit (cat. no. 218076; Qiagen, Inc.) was used in a StepOne Real-Time PCR thermal cycler (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: Small nuclear RNA U6 (forward primer, 5′-CGCTTCGGCAGCACATATACTA-3′; cat. no. MS00033740; Qiagen, Inc.) was used as a miR housekeeping control. .. The reverse primer is included in the miScript SYBR Green qPCR kit (cat. no. 218076; Qiagen, Inc.) and is designed specifically for this purpose. ..

    Polymerase Chain Reaction:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    Sequencing:

    Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression.
    Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using miScript II RT Kit (Qiagen). qRT-PCR analysis of primary (pri-), precursor (pre-), and mature-miR-223 was performed using miScript primer assays (Qiagen), a SYBR Green polymerase chain reaction kit (Qiagen), and ABI Prism 7500 HT sequence detection system (Applied Biosystems, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: The miScript II RT kit (cat. no. 218161; Qiagen, Inc.) was used for the reverse transcription of RNA into cDNA, adhering to the manufacturer's protocol for miRs. .. To detect miR-145 and miR-23b levels, the miScript SYBR Green qPCR Kit (cat. no. 218076; Qiagen, Inc.) was used in a StepOne Real-Time PCR thermal cycler (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: Small nuclear RNA U6 (forward primer, 5′-CGCTTCGGCAGCACATATACTA-3′; cat. no. MS00033740; Qiagen, Inc.) was used as a miR housekeeping control. .. The reverse primer is included in the miScript SYBR Green qPCR kit (cat. no. 218076; Qiagen, Inc.) and is designed specifically for this purpose. ..

    Reverse Transcription:

    Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines
    Article Snippet: Extraction of miRs was carried out using chemical and mechanical lysis using QIAzol lysis reagent (Qiagen, Inc.), followed by the miRNeasy kit (Qiagen, Inc.), according to the manufacturer's instructions. .. The miScript II RT kit (cat. no. 218161; Qiagen, Inc.) was used for the reverse transcription of RNA into cDNA, adhering to the manufacturer's protocol for miRs. .. To detect miR-145 and miR-23b levels, the miScript SYBR Green qPCR Kit (cat. no. 218076; Qiagen, Inc.) was used in a StepOne Real-Time PCR thermal cycler (Applied Biosystems; Thermo Fisher Scientific, Inc.).

    cDNA Synthesis:

    Article Title: Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma
    Article Snippet: .. Complementary DNA (cDNA) synthesis was carried out with 1 μg of miRNA using the miScript II RT Kit (Qiagen GmbH, Germany), adhering to the manufacturer's instructions. .. The expression level of miR-30a-5p was assessed using miR-30a-5p and U6 small RNA (as the endogenous control) miScript primer assays (Qiagen GmbH, Hilden, Germany). miR-30a-5p expression was determined using the miScript SYBR® Green PCR Kit (Qiagen GmbH, Hilden, Germany), following the manufacturer's protocol.



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    Acute SIV infection is associated with altered liver metabolism. Changes in the expression of 84 genes associated with steatotic liver disease were profiled using the RT2 PCR array for fatty liver disease using RNA extracted from 3 SIV-infected (SIV+) and 3 SIV-naïve (SIV−) macaques with liver samples available at the 2-week time point. ( A ) The 3 genes that were different ( p < 0.10) between the SIV+ and SIV− macaques (PPARA, ABCA1, and GSK3B). ( B ) The 3 genes with the greatest fold-change in an alcoholic liver disease model in macaques are also depicted (PPARGC1A, IGFBP1, and FASN) .

    Journal: Viruses

    Article Title: SIV Infection Is Associated with Transient Acute-Phase Steatosis in Hepatocytes In Vivo

    doi: 10.3390/v16020296

    Figure Lengend Snippet: Acute SIV infection is associated with altered liver metabolism. Changes in the expression of 84 genes associated with steatotic liver disease were profiled using the RT2 PCR array for fatty liver disease using RNA extracted from 3 SIV-infected (SIV+) and 3 SIV-naïve (SIV−) macaques with liver samples available at the 2-week time point. ( A ) The 3 genes that were different ( p < 0.10) between the SIV+ and SIV− macaques (PPARA, ABCA1, and GSK3B). ( B ) The 3 genes with the greatest fold-change in an alcoholic liver disease model in macaques are also depicted (PPARGC1A, IGFBP1, and FASN) .

    Article Snippet: Gene expression was analyzed using the RT2 Profiler PCR Array Data Analysis Template 2024 (Qiagen) via the 2 −∆∆Ct method.

    Techniques: Infection, Expressing