Review



mouse isocitrate dehydrogenase [nadp] cytoplasmic (idh1) elisa kit  (Abbexa Ltd)


Bioz Verified Symbol Abbexa Ltd is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Abbexa Ltd mouse isocitrate dehydrogenase [nadp] cytoplasmic (idh1) elisa kit
    ( A ) Characteristics of energy metabolism for ALF patients. ( B ) The expression levels of L-Lactate, L-Malate, <t>Isocitrate,</t> Succinate, 3-phosphoglycerate, ADP, α-Ketoglutarate, ATP, Citrate, Oxaloacetate, NAD, NADPH, Phosphoenolpyruvate, and UDPglucose were significantly different between ALF patients and normal volunteers. ( C ) Based on the Pearson correlation analysis method, the correlation coefficient between different metabolites was calculated. The correlation between the different metabolites was displayed in the form of a correlation coefficient matrix heat map, and the expression of each metabolite was replaced by the ratio of the relative expression of the ALF group to the relative expression of the normal group. This matrix chart shows the correlation between significantly different metabolites. The Pearson correlation coefficient value R was between −1 and +1. The correlation coefficient R between metabolites was represented by color and circle, where R > 0 represents a positive correlation, and it was represented by red. R < 0 indicates a negative correlation, which was represented by blue. The larger and darker the circles, the more relevant they were. It was found that the expression for α-Ketoglutarate, Citrate, and Oxaloacetate was positively correlated with ATP. The expression for L-Malate, Isocitrate, and Succinate was negatively correlated with ATP. ( D ) Hypothesis diagram of M1 macrophage energy metabolism during ALF process.
    Mouse Isocitrate Dehydrogenase [Nadp] Cytoplasmic (Idh1) Elisa Kit, supplied by Abbexa Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+processing+software+idps/pmc08055295-56-0-11?v=Abbexa+Ltd
    Average 90 stars, based on 1 article reviews
    mouse isocitrate dehydrogenase [nadp] cytoplasmic (idh1) elisa kit - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Histone Deacetylase 6 Regulates the Activation of M1 Macrophages by the Glycolytic Pathway During Acute Liver Failure"

    Article Title: Histone Deacetylase 6 Regulates the Activation of M1 Macrophages by the Glycolytic Pathway During Acute Liver Failure

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S302391

    ( A ) Characteristics of energy metabolism for ALF patients. ( B ) The expression levels of L-Lactate, L-Malate, Isocitrate, Succinate, 3-phosphoglycerate, ADP, α-Ketoglutarate, ATP, Citrate, Oxaloacetate, NAD, NADPH, Phosphoenolpyruvate, and UDPglucose were significantly different between ALF patients and normal volunteers. ( C ) Based on the Pearson correlation analysis method, the correlation coefficient between different metabolites was calculated. The correlation between the different metabolites was displayed in the form of a correlation coefficient matrix heat map, and the expression of each metabolite was replaced by the ratio of the relative expression of the ALF group to the relative expression of the normal group. This matrix chart shows the correlation between significantly different metabolites. The Pearson correlation coefficient value R was between −1 and +1. The correlation coefficient R between metabolites was represented by color and circle, where R > 0 represents a positive correlation, and it was represented by red. R < 0 indicates a negative correlation, which was represented by blue. The larger and darker the circles, the more relevant they were. It was found that the expression for α-Ketoglutarate, Citrate, and Oxaloacetate was positively correlated with ATP. The expression for L-Malate, Isocitrate, and Succinate was negatively correlated with ATP. ( D ) Hypothesis diagram of M1 macrophage energy metabolism during ALF process.
    Figure Legend Snippet: ( A ) Characteristics of energy metabolism for ALF patients. ( B ) The expression levels of L-Lactate, L-Malate, Isocitrate, Succinate, 3-phosphoglycerate, ADP, α-Ketoglutarate, ATP, Citrate, Oxaloacetate, NAD, NADPH, Phosphoenolpyruvate, and UDPglucose were significantly different between ALF patients and normal volunteers. ( C ) Based on the Pearson correlation analysis method, the correlation coefficient between different metabolites was calculated. The correlation between the different metabolites was displayed in the form of a correlation coefficient matrix heat map, and the expression of each metabolite was replaced by the ratio of the relative expression of the ALF group to the relative expression of the normal group. This matrix chart shows the correlation between significantly different metabolites. The Pearson correlation coefficient value R was between −1 and +1. The correlation coefficient R between metabolites was represented by color and circle, where R > 0 represents a positive correlation, and it was represented by red. R < 0 indicates a negative correlation, which was represented by blue. The larger and darker the circles, the more relevant they were. It was found that the expression for α-Ketoglutarate, Citrate, and Oxaloacetate was positively correlated with ATP. The expression for L-Malate, Isocitrate, and Succinate was negatively correlated with ATP. ( D ) Hypothesis diagram of M1 macrophage energy metabolism during ALF process.

    Techniques Used: Expressing

    The effect of ACY-1215 on LPS/D-Gal induced ALF mice. ( A ) HE staining was used to detect histopathological changes in liver. TUNEL staining was used to detect the cell apoptosis level. The serum levels of ALT, AST, and TBIL were detected. ( B ) Venn diagram showing the protein quantitative sequencing. ( A ) ACY-1215 intervention group. M: Model group. N: Control group. M/N (up/down) represented the protein whose expression level was increased (decreased) in the model group compared with the normal group. A/M (up/down) represented the protein whose expression level was increased (decreased) in the ACY-1215 intervention group compared with the model group. The table showed different expression levels of MDH1, IDH1 and DNMT1 in different mice groups. ( C ) The substrate and product of MDH1 were L-Malate and Oxaloacetate. The substrate and product of IDH1 were Isocitrate and α-Ketoglutarate. ( D ) The levels of ATP, Oxaloacetate, MDH1, α-Ketoglutarate, Citrate, MDH1, L-Malate, Isocitrate and Succinate in liver were tested. Data are shown as mean ± SD. # P < 0.05, compared with the control group. * P < 0.05, compared with the LPS/D-Gal group.
    Figure Legend Snippet: The effect of ACY-1215 on LPS/D-Gal induced ALF mice. ( A ) HE staining was used to detect histopathological changes in liver. TUNEL staining was used to detect the cell apoptosis level. The serum levels of ALT, AST, and TBIL were detected. ( B ) Venn diagram showing the protein quantitative sequencing. ( A ) ACY-1215 intervention group. M: Model group. N: Control group. M/N (up/down) represented the protein whose expression level was increased (decreased) in the model group compared with the normal group. A/M (up/down) represented the protein whose expression level was increased (decreased) in the ACY-1215 intervention group compared with the model group. The table showed different expression levels of MDH1, IDH1 and DNMT1 in different mice groups. ( C ) The substrate and product of MDH1 were L-Malate and Oxaloacetate. The substrate and product of IDH1 were Isocitrate and α-Ketoglutarate. ( D ) The levels of ATP, Oxaloacetate, MDH1, α-Ketoglutarate, Citrate, MDH1, L-Malate, Isocitrate and Succinate in liver were tested. Data are shown as mean ± SD. # P < 0.05, compared with the control group. * P < 0.05, compared with the LPS/D-Gal group.

    Techniques Used: Staining, TUNEL Assay, Sequencing, Expressing

    The effect of ACY-1215 on energy metabolite in LPS induced RAW264.7 cells. ( A ) The levels of ATP, Oxaloacetate, MDH1, α-Ketoglutarate, Citrate, MDH1, L-Malate, Isocitrate, and Succinate in RAW264.7 cells was tested. ( B – D ) The levels of DDX3X, NLRP3, and DNMT1 were detected by immunofluorescence. ( E ) A histogram was used to show the protein level of DDX3X, NLRP3 and DNMT1. ( F ) Cell apoptosis was detected by flow cytometry. ( G ) The levels of HIF1α, TNF-α, IL-6, and IL-1β in cell were tested by ELISA kits. Data are shown as mean ± SD. # P < 0.05, compared with the control group. * P < 0.05, compared with the LPS group.
    Figure Legend Snippet: The effect of ACY-1215 on energy metabolite in LPS induced RAW264.7 cells. ( A ) The levels of ATP, Oxaloacetate, MDH1, α-Ketoglutarate, Citrate, MDH1, L-Malate, Isocitrate, and Succinate in RAW264.7 cells was tested. ( B – D ) The levels of DDX3X, NLRP3, and DNMT1 were detected by immunofluorescence. ( E ) A histogram was used to show the protein level of DDX3X, NLRP3 and DNMT1. ( F ) Cell apoptosis was detected by flow cytometry. ( G ) The levels of HIF1α, TNF-α, IL-6, and IL-1β in cell were tested by ELISA kits. Data are shown as mean ± SD. # P < 0.05, compared with the control group. * P < 0.05, compared with the LPS group.

    Techniques Used: Immunofluorescence, Flow Cytometry, Enzyme-linked Immunosorbent Assay



    Similar Products

    data processing software idps  (Inscopix Inc)
    86
    Inscopix Inc data processing software idps
    Data Processing Software Idps, supplied by Inscopix Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+processing+software+idps/bio_rxiv__64898__2026__06__02__729661-381-6-5?v=Inscopix+Inc
    Average 86 stars, based on 1 article reviews
    data processing software idps - by Bioz Stars, 2026-07
    86/100 stars
    		  Buy from Supplier
    data processing software (idps)  (Inscopix Inc)
    90
    Inscopix Inc data processing software (idps)
    Data Processing Software (Idps), supplied by Inscopix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+processing+software+idps/pmc12275950-19-1-0?v=Inscopix+Inc
    Average 90 stars, based on 1 article reviews
    data processing software (idps) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    idps (inscopix data processing software)  (Inscopix Inc)
    90
    Inscopix Inc idps (inscopix data processing software)
    Idps (Inscopix Data Processing Software), supplied by Inscopix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+processing+software+idps/pmc12192352-285-15-16?v=Inscopix+Inc
    Average 90 stars, based on 1 article reviews
    idps (inscopix data processing software) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    data processing software idps v1.9.1  (Inscopix Inc)
    90
    Inscopix Inc data processing software idps v1.9.1
    Data Processing Software Idps V1.9.1, supplied by Inscopix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+processing+software+idps/pmc12138423-292-23-23?v=Inscopix+Inc
    Average 90 stars, based on 1 article reviews
    data processing software idps v1.9.1 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    data processing software idps 1.8.0  (Inscopix Inc)
    90
    Inscopix Inc data processing software idps 1.8.0
    a , Simultaneous in vivo calcium imaging and optogenetic stimulation under free-moving conditions. AAV-syn-GCaMP6f and AAV-syn-ChrimsonR were co-injected into the DG, followed by GRIN-lens implantation. An integrated miniature microscope (nVoke; <t>Inscopix)</t> enabled large-scale cellular-resolution Ca 2+ imaging and optogenetic stimulation. b, Left: Expression of GCaMP6f and ChrimsonR in the DG. Right: Max projection of relative fluorescence change (Δ F / F ) of Ca 2+ transients from all imaging frames during the first 30 min, showing representative active neurons. c, Top: Experimental design of Ca 2+ imaging combined with optogenetic stimulation during the open field test. Each 50-min session consisted of 30-min Ca 2+ imaging (used for the following analysis), 5-min optogenetic stimulation, and 15-min post-stimulation recording. This was repeated for 10 days. No light was introduced to mice in the No Stim group. Bottom: Δ F / F traces from 15 representative neurons (scale bar, 10% Δ F / F ). <t>Data</t> from 25 to 38 min of a 50-min session are shown. d, Distance traveled during the first 30 min e, Average Ca 2+ transient rate during the first 30 min. f, Distribution of spatial information for the No Stim (grey curve) and Stim×10 groups (red curve). The vertical axis represents the frequency of distribution, with the dotted line indicating the criterion for place cells (top 95% percentile of the shuffled distribution; see Methods section). Bar graph indicates proportion of place cells. g, Representative results of position decoding using Ca 2+ imaging data. The first 30 min was split into two 15-min halves for training and test data for decoding. Black/red lines: observed position; grey/pink lines: decoded position. h, Decoding accuracy (mae; mean absolute error, cm). Dotted lines: shuffled control. Two-way repeated measures ANOVA: Stim type, F (1, 8) = 6.49, P = 0.034; Day, F (2, 16) = 2.89, P = 0.085; Stim type × Day, F (2, 16) = 1.11, P = 0.353. Bonferroni correction for multiple comparisons was performed, * P < 0.05. i, Same as f , but for speed information and speed cells. The dotted line indicates the criterion for speed cells (top 99% percentile of the shuffled distribution). j, Same as g , but for speed decoding. k, Same as h , but for speed decoding accuracy. Two-way repeated measures ANOVA: Stim type, F (1, 8) =4.02, P = 0.080; Day, F (2, 16) = 4.87, P = 0.022; Day×Stim type, F (2, 16) = 3.98, P = 0.039. Bonferroni correction for multiple comparisons was performed, * P < 0.05.
    Data Processing Software Idps 1.8.0, supplied by Inscopix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+processing+software+idps/bio_rxiv__2025__05__02__651848-361-16-16?v=Inscopix+Inc
    Average 90 stars, based on 1 article reviews
    data processing software idps 1.8.0 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    idps inscopix data 721 processing software  (Inscopix Inc)
    90
    Inscopix Inc idps inscopix data 721 processing software
    a , Simultaneous in vivo calcium imaging and optogenetic stimulation under free-moving conditions. AAV-syn-GCaMP6f and AAV-syn-ChrimsonR were co-injected into the DG, followed by GRIN-lens implantation. An integrated miniature microscope (nVoke; <t>Inscopix)</t> enabled large-scale cellular-resolution Ca 2+ imaging and optogenetic stimulation. b, Left: Expression of GCaMP6f and ChrimsonR in the DG. Right: Max projection of relative fluorescence change (Δ F / F ) of Ca 2+ transients from all imaging frames during the first 30 min, showing representative active neurons. c, Top: Experimental design of Ca 2+ imaging combined with optogenetic stimulation during the open field test. Each 50-min session consisted of 30-min Ca 2+ imaging (used for the following analysis), 5-min optogenetic stimulation, and 15-min post-stimulation recording. This was repeated for 10 days. No light was introduced to mice in the No Stim group. Bottom: Δ F / F traces from 15 representative neurons (scale bar, 10% Δ F / F ). <t>Data</t> from 25 to 38 min of a 50-min session are shown. d, Distance traveled during the first 30 min e, Average Ca 2+ transient rate during the first 30 min. f, Distribution of spatial information for the No Stim (grey curve) and Stim×10 groups (red curve). The vertical axis represents the frequency of distribution, with the dotted line indicating the criterion for place cells (top 95% percentile of the shuffled distribution; see Methods section). Bar graph indicates proportion of place cells. g, Representative results of position decoding using Ca 2+ imaging data. The first 30 min was split into two 15-min halves for training and test data for decoding. Black/red lines: observed position; grey/pink lines: decoded position. h, Decoding accuracy (mae; mean absolute error, cm). Dotted lines: shuffled control. Two-way repeated measures ANOVA: Stim type, F (1, 8) = 6.49, P = 0.034; Day, F (2, 16) = 2.89, P = 0.085; Stim type × Day, F (2, 16) = 1.11, P = 0.353. Bonferroni correction for multiple comparisons was performed, * P < 0.05. i, Same as f , but for speed information and speed cells. The dotted line indicates the criterion for speed cells (top 99% percentile of the shuffled distribution). j, Same as g , but for speed decoding. k, Same as h , but for speed decoding accuracy. Two-way repeated measures ANOVA: Stim type, F (1, 8) =4.02, P = 0.080; Day, F (2, 16) = 4.87, P = 0.022; Day×Stim type, F (2, 16) = 3.98, P = 0.039. Bonferroni correction for multiple comparisons was performed, * P < 0.05.
    Idps Inscopix Data 721 Processing Software, supplied by Inscopix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+processing+software+idps/pm40157920-532-8-9?v=Inscopix+Inc
    Average 90 stars, based on 1 article reviews
    idps inscopix data 721 processing software - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Simultaneous in vivo calcium imaging and optogenetic stimulation under free-moving conditions. AAV-syn-GCaMP6f and AAV-syn-ChrimsonR were co-injected into the DG, followed by GRIN-lens implantation. An integrated miniature microscope (nVoke; Inscopix) enabled large-scale cellular-resolution Ca 2+ imaging and optogenetic stimulation. b, Left: Expression of GCaMP6f and ChrimsonR in the DG. Right: Max projection of relative fluorescence change (Δ F / F ) of Ca 2+ transients from all imaging frames during the first 30 min, showing representative active neurons. c, Top: Experimental design of Ca 2+ imaging combined with optogenetic stimulation during the open field test. Each 50-min session consisted of 30-min Ca 2+ imaging (used for the following analysis), 5-min optogenetic stimulation, and 15-min post-stimulation recording. This was repeated for 10 days. No light was introduced to mice in the No Stim group. Bottom: Δ F / F traces from 15 representative neurons (scale bar, 10% Δ F / F ). Data from 25 to 38 min of a 50-min session are shown. d, Distance traveled during the first 30 min e, Average Ca 2+ transient rate during the first 30 min. f, Distribution of spatial information for the No Stim (grey curve) and Stim×10 groups (red curve). The vertical axis represents the frequency of distribution, with the dotted line indicating the criterion for place cells (top 95% percentile of the shuffled distribution; see Methods section). Bar graph indicates proportion of place cells. g, Representative results of position decoding using Ca 2+ imaging data. The first 30 min was split into two 15-min halves for training and test data for decoding. Black/red lines: observed position; grey/pink lines: decoded position. h, Decoding accuracy (mae; mean absolute error, cm). Dotted lines: shuffled control. Two-way repeated measures ANOVA: Stim type, F (1, 8) = 6.49, P = 0.034; Day, F (2, 16) = 2.89, P = 0.085; Stim type × Day, F (2, 16) = 1.11, P = 0.353. Bonferroni correction for multiple comparisons was performed, * P < 0.05. i, Same as f , but for speed information and speed cells. The dotted line indicates the criterion for speed cells (top 99% percentile of the shuffled distribution). j, Same as g , but for speed decoding. k, Same as h , but for speed decoding accuracy. Two-way repeated measures ANOVA: Stim type, F (1, 8) =4.02, P = 0.080; Day, F (2, 16) = 4.87, P = 0.022; Day×Stim type, F (2, 16) = 3.98, P = 0.039. Bonferroni correction for multiple comparisons was performed, * P < 0.05.

    Journal: bioRxiv

    Article Title: Repetitive Neuronal Activation Regulates Cellular Maturation State via Nuclear Reprogramming

    doi: 10.1101/2025.05.02.651848

    Figure Lengend Snippet: a , Simultaneous in vivo calcium imaging and optogenetic stimulation under free-moving conditions. AAV-syn-GCaMP6f and AAV-syn-ChrimsonR were co-injected into the DG, followed by GRIN-lens implantation. An integrated miniature microscope (nVoke; Inscopix) enabled large-scale cellular-resolution Ca 2+ imaging and optogenetic stimulation. b, Left: Expression of GCaMP6f and ChrimsonR in the DG. Right: Max projection of relative fluorescence change (Δ F / F ) of Ca 2+ transients from all imaging frames during the first 30 min, showing representative active neurons. c, Top: Experimental design of Ca 2+ imaging combined with optogenetic stimulation during the open field test. Each 50-min session consisted of 30-min Ca 2+ imaging (used for the following analysis), 5-min optogenetic stimulation, and 15-min post-stimulation recording. This was repeated for 10 days. No light was introduced to mice in the No Stim group. Bottom: Δ F / F traces from 15 representative neurons (scale bar, 10% Δ F / F ). Data from 25 to 38 min of a 50-min session are shown. d, Distance traveled during the first 30 min e, Average Ca 2+ transient rate during the first 30 min. f, Distribution of spatial information for the No Stim (grey curve) and Stim×10 groups (red curve). The vertical axis represents the frequency of distribution, with the dotted line indicating the criterion for place cells (top 95% percentile of the shuffled distribution; see Methods section). Bar graph indicates proportion of place cells. g, Representative results of position decoding using Ca 2+ imaging data. The first 30 min was split into two 15-min halves for training and test data for decoding. Black/red lines: observed position; grey/pink lines: decoded position. h, Decoding accuracy (mae; mean absolute error, cm). Dotted lines: shuffled control. Two-way repeated measures ANOVA: Stim type, F (1, 8) = 6.49, P = 0.034; Day, F (2, 16) = 2.89, P = 0.085; Stim type × Day, F (2, 16) = 1.11, P = 0.353. Bonferroni correction for multiple comparisons was performed, * P < 0.05. i, Same as f , but for speed information and speed cells. The dotted line indicates the criterion for speed cells (top 99% percentile of the shuffled distribution). j, Same as g , but for speed decoding. k, Same as h , but for speed decoding accuracy. Two-way repeated measures ANOVA: Stim type, F (1, 8) =4.02, P = 0.080; Day, F (2, 16) = 4.87, P = 0.022; Day×Stim type, F (2, 16) = 3.98, P = 0.039. Bonferroni correction for multiple comparisons was performed, * P < 0.05.

    Article Snippet: To extract the activity patterns of individual DG neurons from the obtained fluorescent images, we used Inscopix Data Processing Software (IDPS 1.8.0).

    Techniques: In Vivo, Imaging, Injection, Microscopy, Expressing, Fluorescence, Control