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scanarray microarray analysis system  (Revvity)


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    Structured Review

    Revvity scanarray microarray analysis system
    A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
    Scanarray Microarray Analysis System, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+analysis/pmc04410939-68-16-20?v=Revvity
    Average 90 stars, based on 4 article reviews
    scanarray microarray analysis system - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "MicroRNA-27a Contributes to Rhabdomyosarcoma Cell Proliferation by Suppressing RARA and RXRA"

    Article Title: MicroRNA-27a Contributes to Rhabdomyosarcoma Cell Proliferation by Suppressing RARA and RXRA

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0125171

    A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different microarray slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
    Figure Legend Snippet: A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different microarray slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).

    Techniques Used: Microarray, Labeling, Expressing, Quantitative RT-PCR



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    Image Search Results


    Cytokine expression profile after AAV gene therapy in NHPs Quantification of vitreous cytokines in NHP1 (A) and NHP2 (B) using the LegendPlex NHP Inflammation Panel with levels expressed as fold-change (log 2) in mean fluorescence intensity (MFI) relative to baseline. RE, right eyes; LE, left eye. (C and D) Single-cell transcriptomic analysis of cytokine gene expression among retinal cell types in NHP1 and NHP2, respectively.

    Journal: Molecular Therapy Advances

    Article Title: Single-cell and spatial transcriptomic analyses of gene therapy-associated retinal inflammation in non-human primates

    doi: 10.1016/j.omta.2026.201726

    Figure Lengend Snippet: Cytokine expression profile after AAV gene therapy in NHPs Quantification of vitreous cytokines in NHP1 (A) and NHP2 (B) using the LegendPlex NHP Inflammation Panel with levels expressed as fold-change (log 2) in mean fluorescence intensity (MFI) relative to baseline. RE, right eyes; LE, left eye. (C and D) Single-cell transcriptomic analysis of cytokine gene expression among retinal cell types in NHP1 and NHP2, respectively.

    Article Snippet: Data were analyzed in the LEGENDplex Data Analysis Software (Qognit, BioLegend).

    Techniques: Expressing, Fluorescence, Single Cell, Gene Expression