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ZSGB Biotech anti-quenching blocker containing dapi
Anti Quenching Blocker Containing Dapi, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi-containing+blocker/dapi/pmc11005799-83-2-7
Average 90 stars, based on 1 article reviews
anti-quenching blocker containing dapi - by Bioz Stars, 2026-09
90/100 stars

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other:

Article Title: Wnt/β-catenin regulates Gli1 + osteogenic progenitors in condylar subchondral bone development and osteoarthritis
Article Snippet: DAPI (ZSGB-BIO, ZLI-9557) was used for nuclear staining.

Article Title: Neural stem cell changes and spatial distribution of AQP4 expression in a fetal goat model of obstructive hydrocephalus
Article Snippet: After rinsing with phosphate-buffered saline (PBS), the slices were incubated with secondary antibodies Alexa Fluor 488 or Alexa Fluor 555 (RRID: AB_10695356, AB_10695726, AB_10693782; Cell Signaling Technology, Massachusetts, United States, 1:1000) at room temperature for 1 h. Finally, the sections were rinsed in PBS and incubated with 4′,6-diamidino-2-phenylindole (DAPI, ZSGB-BIO, China) for nuclear staining.

Article Title: Antibacterial effects and mechanisms of graphene oxide loaded on TiO 2 -nanotube-modified ti: an in vitro study.
Article Snippet: The samples were rinsed with PBS three times and stained with DAPI (ZLI-9557, ZSGB-BIO, Beijing, China).

Incubation:

Article Title: Comparison of Efavirenz and Dolutegravir on Gut Microbiome and Gut Barrier Functions
Article Snippet: .. After deparaffinization and antigen retrieval (EDTA, pH 8.0), immunofluorescent detection of the tight junction protein Zonula occludens-1 (ZO-1) was performed with rabbit anti-ZO-1 polyantibody (21773–1-AP, Proteintech, China) incubation at 4 °C overnight, followed with Alexa Fluor 594-conjugated goat antirabbit lgG (H+L) (ZSGB-BIO, Beijing, China) incubation at 37 °C for 1 h. After the coverslip was mounted with antifade mountant containing DAPI (ZSGB-BIO), the slides were observed and photographed under a Leica TCS SP8 laser confocal microscope with LAS X Life Science Microscope Software (USA). ..

Article Title: Inhibition of Axl attenuates acute kidney injury by alleviating inflammation via SOCS3 downregulation in tubular epithelial cells.
Article Snippet: Cell slides were incubated with anti-Ki67 (1:300, Cat# 9129; CST) overnight at 4°C. .. After three PBS washes, sections and slides were incubated with secondary antibodies, washed, and mounted with a fluorescent medium containing 4’,6-diamidino2-phenylindole (Cat# ZLI-9557; ZSGB-BIO). .. Staining was visualized using a fluorescence microscope (Leica).

Article Title: SOX6 enhances vascular smooth muscle cell phenotypic switching and elevates blood pressure by activating autophagy.
Article Snippet: For Masson's trichrome staining, sections were processed using Masson's Trichrome Stain Kit (G1340, SolarBio) following the manufacturer's instructions. .. For cell staining, cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X- 100 for 10 min, and blocked with 5% BSA for 1 h. Primary antibodies (Sox6, 1:500, ab314210, Abcam; LC3, 1:500, ab48394, Abcam) were incubated overnight at 4°C, followed by incubation with a mixture of secondary fluorescent antibodies (1:1000, ab150077, Abcam) at room temperature for 1 h. Sections were mounted with 4′,6- diamidino- 2- phenylindole (DAPI, ZLI- 9600, ZSGB- BIO). ..

Microscopy:

Article Title: Comparison of Efavirenz and Dolutegravir on Gut Microbiome and Gut Barrier Functions
Article Snippet: .. After deparaffinization and antigen retrieval (EDTA, pH 8.0), immunofluorescent detection of the tight junction protein Zonula occludens-1 (ZO-1) was performed with rabbit anti-ZO-1 polyantibody (21773–1-AP, Proteintech, China) incubation at 4 °C overnight, followed with Alexa Fluor 594-conjugated goat antirabbit lgG (H+L) (ZSGB-BIO, Beijing, China) incubation at 37 °C for 1 h. After the coverslip was mounted with antifade mountant containing DAPI (ZSGB-BIO), the slides were observed and photographed under a Leica TCS SP8 laser confocal microscope with LAS X Life Science Microscope Software (USA). ..

Software:

Article Title: Comparison of Efavirenz and Dolutegravir on Gut Microbiome and Gut Barrier Functions
Article Snippet: .. After deparaffinization and antigen retrieval (EDTA, pH 8.0), immunofluorescent detection of the tight junction protein Zonula occludens-1 (ZO-1) was performed with rabbit anti-ZO-1 polyantibody (21773–1-AP, Proteintech, China) incubation at 4 °C overnight, followed with Alexa Fluor 594-conjugated goat antirabbit lgG (H+L) (ZSGB-BIO, Beijing, China) incubation at 37 °C for 1 h. After the coverslip was mounted with antifade mountant containing DAPI (ZSGB-BIO), the slides were observed and photographed under a Leica TCS SP8 laser confocal microscope with LAS X Life Science Microscope Software (USA). ..

Staining:

Article Title:
Article Snippet: .. Mounting medium with DAPI (ZSGB-BIO, ZLI-9557, China) was applied for nuclear staining. .. Sections were imaged through a Leica TCS SP8 STED confocal fluorescent microscope (3X, Germany).

Article Title: SOX6 enhances vascular smooth muscle cell phenotypic switching and elevates blood pressure by activating autophagy.
Article Snippet: For Masson's trichrome staining, sections were processed using Masson's Trichrome Stain Kit (G1340, SolarBio) following the manufacturer's instructions. .. For cell staining, cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X- 100 for 10 min, and blocked with 5% BSA for 1 h. Primary antibodies (Sox6, 1:500, ab314210, Abcam; LC3, 1:500, ab48394, Abcam) were incubated overnight at 4°C, followed by incubation with a mixture of secondary fluorescent antibodies (1:1000, ab150077, Abcam) at room temperature for 1 h. Sections were mounted with 4′,6- diamidino- 2- phenylindole (DAPI, ZLI- 9600, ZSGB- BIO). ..



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