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Structured Review

SERVA Electrophoresis dapi no. 18860
Dapi No. 18860, supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+stock+solution/dapi/pmc11704138-340-30-45
Average 90 stars, based on 1 article reviews
dapi no. 18860 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: Repetitive prefrontal tDCS activates VTA dopaminergic neurons, resulting in attenuation of Alzheimer's Disease-like deficits in Tg2576 mice.
Article Snippet: For Aβ/Iba1 immunostaining, sections were pretreated with M.O.M.® (Mouse on Mouse) Blocking Reagent (1:1000; Vector laboratories, #MKB-2213-1, 2 h, RT) diluted in permeabilization solution (PB with 0.3% Triton X-100), and then incubated with primary antibodies overnight at 4 °C in permeabilization solution. .. Sections were subsequently washed in PB and incubated with secondary antibodies in the permeabilization solution (2 h, RT) followed by washes in PB and incubation with DAPI (1:1000, Serva). .. After mounted, slices were examined using a Nikon Eclipse Ti2 confocal microscope.

Staining:

Article Title: Evaluation of the Expression of IDO and PTEN in Human Kidney Cancer
Article Snippet: .. Then, 0.5 μg/mL DAPI (SERVA Electrophoresis GmbH, Heidelberg, Germany) was added for nuclear staining. .. The fluorescent signal was detected using a ZEISS Axioscope fluorescent microscope (ZEISS, Oberkochen, Germany).

Expressing:

Article Title: Editing B cells at the IGHA2 gene position provides alternative route to therapeutic IgA production.
Article Snippet: As professional and long-lived immunoglobulin (Ig) producers, B cells represent attractive candidates for adoptive immunotherapy and their highly expressed Ig heavy (IgH) chain locus is ideal for editing.. Each of its constant genes, expressed after class switch recombination (CSR), affords an attractive platform where an adoptive Ig variable domain would acquire IgM, IgG, IgE or IgA class-specific functions.. In particular, IgA plays a unique role in mucosal immunity but has remained excluded from therapeutic applicability due to unfavorable chemistry, manufacturing, and control (CMC) issues.

other:

Article Title: Animal-Free Setup of a 3D Mature Adipocyte-Macrophage Co-Culture to Induce Inflammation In Vitro.
Article Snippet: Gels were washed and counterstained with 4’, 6-diamidino-2-phenylindole (DAPI, 1μg mL−1, Serva Electrophoresis) for nuclei staining.

Article Title: Chronic palmitoylethanolamide administration via slow-release subcutaneous pellets promotes neuroprotection and mitigates neuroinflammation in the Tg2576 mouse model of Alzheimer’s disease
Article Snippet: All sections were counterstained with DAPI (1:2000; #18860.01; Serva, Germany) for 5 min in PB and mounted using an anti-fade medium (Fluoromount, Sigma-Aldrich, MO, USA).

Article Title: Interplay of kinetochores and catalysts drives rapid assembly of the mitotic checkpoint complex.
Article Snippet: Subsequently, cells were incubated for 1 h sat room temperature with the following secondary antibodies: anti-MAD1-DyLight550 (mouse, in-house generated, 1:500), goat anti-human Alexa Fluor 488 (Invitrogen, Carlsbad, California, USA, 1:200), goat anti-human Rodamine Red (Jackson Immunoresearch, 1:200).

Cytometry:

Article Title: Editing B cells at the IGHA2 gene position provides alternative route to therapeutic IgA production.
Article Snippet: As professional and long-lived immunoglobulin (Ig) producers, B cells represent attractive candidates for adoptive immunotherapy and their highly expressed Ig heavy (IgH) chain locus is ideal for editing.. Each of its constant genes, expressed after class switch recombination (CSR), affords an attractive platform where an adoptive Ig variable domain would acquire IgM, IgG, IgE or IgA class-specific functions.. In particular, IgA plays a unique role in mucosal immunity but has remained excluded from therapeutic applicability due to unfavorable chemistry, manufacturing, and control (CMC) issues.



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