Review




Structured Review

Merck KGaA dapi staining solution d8471
Autophagy promotes the apoptosis of U87 and C6 cells. (A) <t>DAPI</t> <t>staining</t> images of U87 and C6 cells were captured using a fluorescence microscope following treatment with or without DRM (15 µ M) in the absence or presence of CQ (15 µ M) for 48h. Scale bar, 50 µ m. (B) U87 and C6 cells were treated with or without DRM (15 µ M) in the absence or presence of CQ (15 µ M) for 48 h, and the apoptotic cell ratio was measured by flow cytometry. (C) Graphical representation of quantitative analysis of the apoptotic rate. The results are presented as the mean ± SD of at least three independent experiments. ** P<0.01. CQ, chloroquine; CTR, control; DRM, doramectin.
Dapi Staining Solution D8471, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution/dapi+staining+solution+d8471/pmc08857930-90-21-25
Average 90 stars, based on 1 article reviews
dapi staining solution d8471 - by Bioz Stars, 2026-09
90/100 stars

Images

1) Product Images from "Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo"

Article Title: Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo

Journal: International Journal of Oncology

doi: 10.3892/ijo.2022.5319

Autophagy promotes the apoptosis of U87 and C6 cells. (A) DAPI staining images of U87 and C6 cells were captured using a fluorescence microscope following treatment with or without DRM (15 µ M) in the absence or presence of CQ (15 µ M) for 48h. Scale bar, 50 µ m. (B) U87 and C6 cells were treated with or without DRM (15 µ M) in the absence or presence of CQ (15 µ M) for 48 h, and the apoptotic cell ratio was measured by flow cytometry. (C) Graphical representation of quantitative analysis of the apoptotic rate. The results are presented as the mean ± SD of at least three independent experiments. ** P<0.01. CQ, chloroquine; CTR, control; DRM, doramectin.
Figure Legend Snippet: Autophagy promotes the apoptosis of U87 and C6 cells. (A) DAPI staining images of U87 and C6 cells were captured using a fluorescence microscope following treatment with or without DRM (15 µ M) in the absence or presence of CQ (15 µ M) for 48h. Scale bar, 50 µ m. (B) U87 and C6 cells were treated with or without DRM (15 µ M) in the absence or presence of CQ (15 µ M) for 48 h, and the apoptotic cell ratio was measured by flow cytometry. (C) Graphical representation of quantitative analysis of the apoptotic rate. The results are presented as the mean ± SD of at least three independent experiments. ** P<0.01. CQ, chloroquine; CTR, control; DRM, doramectin.

Techniques Used: Staining, Fluorescence, Microscopy, Flow Cytometry, Control

Related Articles

Staining:

Article Title: Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo
Article Snippet: .. The cells were harvested, washed twice with PBS, fixed with 4% formaldehyde for 10 min at room temperature and stained with DAPI (cat. no. D8471; Merck KGaA) staining solution according to the manufacturer's instructions for 10 min at 37°C. .. The images were immediately captured using a fluorescence microscope (Olympus Corporation).



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