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acdbio dapi  (Advanced Cell Diagnostics Inc)


Bioz Verified Symbol Advanced Cell Diagnostics Inc is a verified supplier
Bioz Manufacturer Symbol Advanced Cell Diagnostics Inc manufactures this product  
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  • 99

    Structured Review

    Advanced Cell Diagnostics Inc acdbio dapi
    Acdbio Dapi, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/DAPI+solution/pm42735685-1158-5-5
    Average 99 stars, based on 119 article reviews
    acdbio dapi - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Itgb3 Expression is Developmentally Regulated in Pyramidal Neurons of Mouse Cortex and Hippocampus
    Article Snippet: For double labeled experiments, we utilized mice ages P7-P14, and a second probe, such as Grm5 (ACD Bio, cat. #423631), Gria2 (ACD Bio, cat. #416091-C3), or Fmr1 (ACD Bio, cat. #496391-C2), was used with TSA Vivid 570 (ACD Bio, cat. #7535) after Itgb3 was detected utilizing TSA Vivid 650. .. After fluorescent detection of mRNAs, all tissue was incubated with DAPI solution (ACD Bio) for 30 seconds, excess DAPI was tapped off, the tissue was coated with ProLong Diamond (Invitrogen) and covered with a #1.5H thickness coverslip. ..

    Article Title: Amygdalostriatal transition zone neurons encode sustained cue responses to guide defensive behaviors.
    Article Snippet: .. Slides were then incubated in ACDbio DAPI for 10 minutes, washed, dried for 20 minutes, coverslipped with PVA-DABCO (Millipore-Sigma), and left to dry overnight before imaging. .. Confocal microscopy For the Drd1a/Drd2 labelling experiment, confocal fluorescence images were acquired on an Olympus FV1000 confocal laser scanning microscope using a 40x/1.30NA oil immersion objective, and serial Z-stack images were acquired using the FluoView software (Olympus, Center Valley, PA) at a thickness of 1.5 μm per Z-slice, with only the best-targeted Z-slice used for each target area.

    Imaging:

    Article Title: Amygdalostriatal transition zone neurons encode sustained cue responses to guide defensive behaviors.
    Article Snippet: .. Slides were then incubated in ACDbio DAPI for 10 minutes, washed, dried for 20 minutes, coverslipped with PVA-DABCO (Millipore-Sigma), and left to dry overnight before imaging. .. Confocal microscopy For the Drd1a/Drd2 labelling experiment, confocal fluorescence images were acquired on an Olympus FV1000 confocal laser scanning microscope using a 40x/1.30NA oil immersion objective, and serial Z-stack images were acquired using the FluoView software (Olympus, Center Valley, PA) at a thickness of 1.5 μm per Z-slice, with only the best-targeted Z-slice used for each target area.

    other:

    Article Title: A dual pronged approach to cancer immune exclusion: tumor-derived LAIR-1 simultaneously drives fibrosis and blocks immune recruitment in glioma
    Article Snippet: RNAscope in-situ hybridization was performed on 5-μm coronal cryosections mounted onto positively charged slides, following the manufacturer’s protocol (ACDBio).

    Article Title: Ependymomas are cancers of the pre-neural crest/roof plate lineage
    Article Snippet: DAB solution (DAKO Omnis) was applied for approximately 2-3 minutes before washing and hematoxylin staining for one minute.

    Amplification:

    Article Title: Age-related loss of brain demyelinating and remyelinating potential is overcome by microglia renewal
    Article Snippet: Tissue then was incubated with a probe against Proteolipid Protein ( Plp , 1,057,381-C2, ACD, MA), diluted 1:2000 in TSA buffer for 2 h at 40 °C. .. This was followed by a three-probe amplification steps (AMP1-AMP3), fluorophore conjugation (Opal 570) and DAPI counterstaining (ACD, CA). .. To quantify cells labeled by RNAscope for Proteolipid Protein (PLP) or microglial cells labeled by mac-3, fluorescent stainings were scanned with the Vectra Polaris Automated Quantitative Pathology Imaging System from Perkin Elmer and quantified semi-automatically with QuPath software.

    Conjugation Assay:

    Article Title: Age-related loss of brain demyelinating and remyelinating potential is overcome by microglia renewal
    Article Snippet: Tissue then was incubated with a probe against Proteolipid Protein ( Plp , 1,057,381-C2, ACD, MA), diluted 1:2000 in TSA buffer for 2 h at 40 °C. .. This was followed by a three-probe amplification steps (AMP1-AMP3), fluorophore conjugation (Opal 570) and DAPI counterstaining (ACD, CA). .. To quantify cells labeled by RNAscope for Proteolipid Protein (PLP) or microglial cells labeled by mac-3, fluorescent stainings were scanned with the Vectra Polaris Automated Quantitative Pathology Imaging System from Perkin Elmer and quantified semi-automatically with QuPath software.



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