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dapi solution  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science dapi solution
    Dapi Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution+c0065/dapi/pmc10891091-263-5-7
    Average 90 stars, based on 1 article reviews
    dapi solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Two novel phosphatidylinositol phosphate-binding proteins are identified in Neospora caninum with NcPX1 playing a crucial role.
    Article Snippet: Neospora caninum is a unicellular parasite that causes neosporosis, a major contributor to reproductive disorders in livestock.. Whether phosphatidylinositol phosphate (PIP)-binding proteins play a key role in the life cycle of the parasite is unknown.. To investigate the functional roles of PIP-binding proteins in the intracellular parasitism and pathogenicity of N. caninum.

    SYBR Green Assay:

    Article Title: Dual-Endogenous Activation and Self-Reinforcing DNA Nanodevice for Highly Efficient and Specific Imaging of MicroRNA in Living Cells
    Article Snippet: Tumor-specific microRNA (miRNA) imaging strategies are essential for studying mechanisms involved in cancer progression.. However, their selectivity and detection effectiveness are often limited by nonspecific signal leakage and false-positive signals.. In this study, a dual-endogenous activation and self-reinforcing target nucleic acid probing (DTNP) system were designed for spatially specific and high-contrast miRNA imaging in living cells.

    Blocking Assay:

    Article Title: Substrate topography-induced osteogenesis of bone marrow stem cells by reducing the chromatin accessibility of YBX1.
    Article Snippet: After being washed twice with PBS, the samples were permeated with 0.1% Triton X-100 (Solarbio) for 10 min. .. Following a blocking step with 5% BSA (Solarbio) for 60 min, both flat and random PLLA membranes were stained with 100 nM TRITC phalloidin (Solarbio) and counterstained with 10 μg/mL DAPI (Solarbio). .. Finally, the samples were examined with a confocal laser scanning microscope (Leica).

    Article Title: Construction of vascularized liver microtissues recapitulates angiocrine-mediated hepatocytes maturation and enhances therapeutic efficacy for acute liver failure
    Article Snippet: .. After permeabilizing in Triton-X100 for 30 min and blocking with 5 % BSA for 1 h at RT, the samples were incubated with diluted primary antibody at 4 °C for 48 h. After washing in PBST, samples were incubated with secondary antibody at 4 °C for 48 h and the nuclear was stained with DAPI (Solarbio, China). .. All images were acquired using a Total internal reflection fluorescent microscope TIRF&Thunder (Leica, Germany).

    Staining:

    Article Title: Substrate topography-induced osteogenesis of bone marrow stem cells by reducing the chromatin accessibility of YBX1.
    Article Snippet: After being washed twice with PBS, the samples were permeated with 0.1% Triton X-100 (Solarbio) for 10 min. .. Following a blocking step with 5% BSA (Solarbio) for 60 min, both flat and random PLLA membranes were stained with 100 nM TRITC phalloidin (Solarbio) and counterstained with 10 μg/mL DAPI (Solarbio). .. Finally, the samples were examined with a confocal laser scanning microscope (Leica).

    Article Title: Construction of vascularized liver microtissues recapitulates angiocrine-mediated hepatocytes maturation and enhances therapeutic efficacy for acute liver failure
    Article Snippet: .. After permeabilizing in Triton-X100 for 30 min and blocking with 5 % BSA for 1 h at RT, the samples were incubated with diluted primary antibody at 4 °C for 48 h. After washing in PBST, samples were incubated with secondary antibody at 4 °C for 48 h and the nuclear was stained with DAPI (Solarbio, China). .. All images were acquired using a Total internal reflection fluorescent microscope TIRF&Thunder (Leica, Germany).

    Incubation:

    Article Title: Construction of vascularized liver microtissues recapitulates angiocrine-mediated hepatocytes maturation and enhances therapeutic efficacy for acute liver failure
    Article Snippet: .. After permeabilizing in Triton-X100 for 30 min and blocking with 5 % BSA for 1 h at RT, the samples were incubated with diluted primary antibody at 4 °C for 48 h. After washing in PBST, samples were incubated with secondary antibody at 4 °C for 48 h and the nuclear was stained with DAPI (Solarbio, China). .. All images were acquired using a Total internal reflection fluorescent microscope TIRF&Thunder (Leica, Germany).

    TUNEL Assay:

    Article Title: Cardiac-targeted FGF4 encapsulated nanoliposomes improve acute myocardial injury induced by ischemia-reperfusion and adriamycin in in vitro and in vivo models
    Article Snippet: BSA, ECL, PVDF membrane, Tween 20, 1.5 M Tris-HCl buffer (pH 6.8), 4 % paraformaldehyde, and Triton X-100 were obtained from Solarbio (Beijing, China). .. DAPI, TUNEL apoptosis detection kit, and PBS buffer were purchased from Solarbio (Beijing, China). .. IL-1β ELISA Kit (PI305), Mouse IL-1β ELISA Kit (PI301), Mouse IL-6 ELISA Kit (PI326), and Mouse TNF-α ELISA Kit (PT512) were acquired from Beyotime (Shanghai, China).



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