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Nacalai dapi solution(1 mg/ml)
Dapi Solution(1 Mg/Ml), supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+solution(1+mg/dapi+solution/pmc11760323-27-0-4
Average 90 stars, based on 1 article reviews
dapi solution(1 mg/ml) - by Bioz Stars, 2026-09
90/100 stars

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other:

Article Title: Hinokitiol exhibits potent anticancer effects in human oral squamous cell carcinoma by inducing apoptosis and inhibition of cell migration
Article Snippet: Objective: Hinokitiol (HNK) has been shown to have various pharmacological effects, including anti-inflammatory and anti-bacterial activities, against various diseases.. However, its effects on oral cancer have not been reported.. Therefore, we investigated the anti-cancer activity and mechanism of action of HNK in oral squamous cell

Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
Article Snippet: DAPI solution(1 mg/mL) , Nacalai Tesque , Cat#19178-91.

Article Title: Controlled aggregative assembly to form self-organizing macroscopic human intestine from induced pluripotent stem cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant murine EGF Peprotech Cat#31509 Recombinant mouse Noggin R&D Cat#1967 Recombinant mouse R-spondin1 R&D Cat#3474-RS A83-01 Tocris Cat#2939 Recombinant Human IGF-1 BioLegend Cat#590906 Nicotinamide Sigma Cat#N0636 Afamin/Wnt3a conditioned medium MBL lifescience Cat#J2-001 Activin A Nacalai Tesque Cat#18585-81 CHIR99021 Cayman Chemicals Cat#13122 Recombinant human FGF4 Peprotech Cat#100-31 Recombinant human BMP-4 R&D Cat# 314-BP Wnt-C59 Chemscene LLC Cat#CS-1420 PIK90 Cayman Chemicals Cat#10010749 Retinoic acid Sigma Aldrich Cat#R2625 Purmorphamine Tocris Bioscience Cat#4551 Y-27632 Fujifilm Wako Pure Chemical Cat#253-00513 FGF-basic(154a.a.), Human, Recombinant Peprotech Cat#100-18B Recombinant Human IGF-I BioLegend Cat#590906 Cellartis DEF-CSTM 500 culture system Takara Bio Cat#Y50101 Advanced DMEM/F12 Thermo Fisher Cat#12634010 DMEM, high glucose Thermo Fisher Cat#11965-092 RPMI 1640 with L-Gln, liquid Nacalai Tesque Cat#30264-85 Embryonic stem cell Fetal Bovine Serum Thermo Fisher Cat#16141079 GlutaMAXTM Supplement Thermo Fisher Cat#35050-061 HEPES buffer Nacalai Tesque Cat#17557-94 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#26253-84 Matrigel Growth Factor Reduced Corning Cat#356231 N-2 supplement (100X) Thermo Fisher Cat#17502-048 B-27 Supplement (50X), serum free Thermo Fisher Cat#17504-044 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat#12587010 TrypLE Express Enzyme Thermo Fisher Cat#12604013 Accumax Nacalai Tesque Cat#17087-54 Hoechst 33342 Thermo Fisher Cat#H3570 VECTASHIELD mounting medium with DAPI VECTOR Laboratories Cat#H1200 DAPI solution (1 mg/mL) Nacalai Tesque Cat#19178-91 The P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 Critical commercial assays RNAscope 2.5HD Assay-Red Advanced Cell Diagnostics Cat#322350 RNeasy Mini kit QIAGEN Cat#74106 QuantiTect Reverse Transcription Kit QIAGEN Cat#205313 Experimental models: Cell lines Human induced pluripotent stem cell line: HiPS-RIKEN-2F RIKEN BRC Cell Bank (Japan) HPS0014 Human induced pluripotent stem cell line: PB001 Kindly gifted by Dr Hideki Masaki (Institute of Medical Science, the University of Tokyo) N/A Human induced pluripotent stem cell line: TkdA3-4 Institute of Medical Science, the University of Tokyo N/A (Continued on next page) Cell Reports Methods 4, 100930, December 16, 2024 e2

Article Title: Controlled aggregative assembly to form self-organizing macroscopic human intestine from induced pluripotent stem cells
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant murine EGF Peprotech Cat#31509 Recombinant mouse Noggin R&D Cat#1967 Recombinant mouse R-spondin1 R&D Cat#3474-RS A83-01 Tocris Cat#2939 Recombinant Human IGF-1 BioLegend Cat#590906 Nicotinamide Sigma Cat#N0636 Afamin/Wnt3a conditioned medium MBL lifescience Cat#J2-001 Activin A Nacalai Tesque Cat#18585-81 CHIR99021 Cayman Chemicals Cat#13122 Recombinant human FGF4 Peprotech Cat#100-31 Recombinant human BMP-4 R&D Cat# 314-BP Wnt-C59 Chemscene LLC Cat#CS-1420 PIK90 Cayman Chemicals Cat#10010749 Retinoic acid Sigma Aldrich Cat#R2625 Purmorphamine Tocris Bioscience Cat#4551 Y-27632 Fujifilm Wako Pure Chemical Cat#253-00513 FGF-basic(154a.a.), Human, Recombinant Peprotech Cat#100-18B Recombinant Human IGF-I BioLegend Cat#590906 Cellartis DEF-CSTM 500 culture system Takara Bio Cat#Y50101 Advanced DMEM/F12 Thermo Fisher Cat#12634010 DMEM, high glucose Thermo Fisher Cat#11965-092 RPMI 1640 with L-Gln, liquid Nacalai Tesque Cat#30264-85 Embryonic stem cell Fetal Bovine Serum Thermo Fisher Cat#16141079 GlutaMAXTM Supplement Thermo Fisher Cat#35050-061 HEPES buffer Nacalai Tesque Cat#17557-94 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#26253-84 Matrigel Growth Factor Reduced Corning Cat#356231 N-2 supplement (100X) Thermo Fisher Cat#17502-048 B-27 Supplement (50X), serum free Thermo Fisher Cat#17504-044 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat#12587010 TrypLE Express Enzyme Thermo Fisher Cat#12604013 Accumax Nacalai Tesque Cat#17087-54 Hoechst 33342 Thermo Fisher Cat#H3570 VECTASHIELD mounting medium with DAPI VECTOR Laboratories Cat#H1200 DAPI solution (1 mg/mL) Nacalai Tesque Cat#19178-91 The P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 Critical commercial assays RNAscope 2.5HD Assay-Red Advanced Cell Diagnostics Cat#322350 RNeasy Mini kit QIAGEN Cat#74106 QuantiTect Reverse Transcription Kit QIAGEN Cat#205313 Experimental models: Cell lines Human induced pluripotent stem cell line: HiPS-RIKEN-2F RIKEN BRC Cell Bank (Japan) HPS0014 Human induced pluripotent stem cell line: PB001 Kindly gifted by Dr Hideki Masaki (Institute of Medical Science, the University of Tokyo) N/A Human induced pluripotent stem cell line: TkdA3-4 Institute of Medical Science, the University of Tokyo N/A (Continued on next page) Cell Reports Methods 4, 100930, December 16, 2024 e2 Report ll OPEN ACCESS

Staining:

Article Title: Direct analysis of hepatic stellate cells with flow cytometry in specimens derived from the human liver
Article Snippet: .. Finally, a DAPI solution (Nacalai, 1:1000) was added for nuclear staining. .. The cells were observed using BZ-X800 (KEYENCE).

Laser-Scanning Microscopy:

Article Title: Unravelling the cardioprotective effects of calcitriol in Sunitinib-induced toxicity: A comprehensive in silico and in vitro study.
Article Snippet: Sunitinib (SUN), a drug used to treat advanced renal cell carcinoma and other cancers, causes cardiotoxicity.. This study aimed to identify a potential drug candidate to counteract SUN-induced cardiotoxicity.. We analysed realworld data from adverse event report databases of existing clinically approved drugs to identify potential candidates.



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Image Search Results


Journal: eLife

Article Title: FABP4-mediated lipid accumulation and lipolysis in tumor-associated macrophages promote breast cancer metastasis

doi: 10.7554/eLife.101221

Figure Lengend Snippet:

Article Snippet: Chemical compound, drug , DAPI solution (1 mg/mL) , Invitrogen , Cat#62248 , 1:1000.

Techniques: Concentration Assay, Staining, Saline, SYBR Green Assay, Red Blood Cell Lysis, Cell Culture, XF Assay, Reverse Transcription, Polymer, Plasmid Preparation, Blocking Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Transfection, Recombinant, Microarray, Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Software, Sterility, Migration, Pore Size, Flow Cytometry, Quantitative RT-PCR, Imaging, Microscopy, Transmission Assay, Electron Microscopy

Journal: PLOS Pathogens

Article Title: Pregnancy trained decidual NK cells protect pregnancies from harmful Fusobacterium nucleatum infection

doi: 10.1371/journal.ppat.1011923

Figure Lengend Snippet:

Article Snippet: DAPI Solution (1 mg/mL) / 1 mL , Sigma-Aldrich , TS-62248.

Techniques: Purification, Virus, Recombinant