other:Article Title: Hinokitiol exhibits potent anticancer effects in human oral squamous cell carcinoma by inducing apoptosis and inhibition of cell migration
Article Snippet: Objective: Hinokitiol (HNK) has been shown to have various pharmacological effects, including anti-inflammatory and anti-bacterial activities, against various diseases.. However, its effects on oral cancer have not been reported.. Therefore, we investigated the anti-cancer activity and mechanism of action of HNK in oral squamous cell
Article Title: Controlled aggregative assembly to form self-organizing macroscopic human intestine from induced pluripotent stem cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant murine EGF Peprotech Cat#31509 Recombinant mouse Noggin R&D Cat#1967 Recombinant mouse R-spondin1 R&D Cat#3474-RS A83-01 Tocris Cat#2939 Recombinant Human IGF-1 BioLegend Cat#590906 Nicotinamide Sigma Cat#N0636 Afamin/Wnt3a conditioned medium MBL lifescience Cat#J2-001 Activin A Nacalai Tesque Cat#18585-81 CHIR99021 Cayman Chemicals Cat#13122 Recombinant human FGF4 Peprotech Cat#100-31 Recombinant human BMP-4 R&D Cat# 314-BP Wnt-C59 Chemscene LLC Cat#CS-1420 PIK90 Cayman Chemicals Cat#10010749 Retinoic acid Sigma Aldrich Cat#R2625 Purmorphamine Tocris Bioscience Cat#4551 Y-27632 Fujifilm Wako Pure Chemical Cat#253-00513 FGF-basic(154a.a.), Human, Recombinant Peprotech Cat#100-18B Recombinant Human IGF-I BioLegend Cat#590906 Cellartis DEF-CSTM 500 culture system Takara Bio Cat#Y50101 Advanced DMEM/F12 Thermo Fisher Cat#12634010 DMEM, high glucose Thermo Fisher Cat#11965-092 RPMI 1640 with L-Gln, liquid Nacalai Tesque Cat#30264-85 Embryonic stem cell Fetal Bovine Serum Thermo Fisher Cat#16141079 GlutaMAXTM Supplement Thermo Fisher Cat#35050-061 HEPES buffer Nacalai Tesque Cat#17557-94 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#26253-84 Matrigel Growth Factor Reduced Corning Cat#356231 N-2 supplement (100X) Thermo Fisher Cat#17502-048 B-27 Supplement (50X), serum free Thermo Fisher Cat#17504-044 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat#12587010 TrypLE Express Enzyme Thermo Fisher Cat#12604013 Accumax Nacalai Tesque Cat#17087-54 Hoechst 33342 Thermo Fisher Cat#H3570 VECTASHIELD mounting medium with DAPI VECTOR Laboratories Cat#H1200 DAPI solution (1 mg/mL) Nacalai Tesque Cat#19178-91 The P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 Critical commercial assays RNAscope 2.5HD Assay-Red Advanced Cell Diagnostics Cat#322350 RNeasy Mini kit QIAGEN Cat#74106 QuantiTect Reverse Transcription Kit QIAGEN Cat#205313 Experimental models: Cell lines Human induced pluripotent stem cell line: HiPS-RIKEN-2F RIKEN BRC Cell Bank (Japan) HPS0014 Human induced pluripotent stem cell line: PB001 Kindly gifted by Dr Hideki Masaki (Institute of Medical Science, the University of Tokyo) N/A Human induced pluripotent stem cell line: TkdA3-4 Institute of Medical Science, the University of Tokyo N/A (Continued on next page) Cell Reports Methods 4, 100930, December 16, 2024 e2
Article Title: Controlled aggregative assembly to form self-organizing macroscopic human intestine from induced pluripotent stem cells
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant murine EGF Peprotech Cat#31509 Recombinant mouse Noggin R&D Cat#1967 Recombinant mouse R-spondin1 R&D Cat#3474-RS A83-01 Tocris Cat#2939 Recombinant Human IGF-1 BioLegend Cat#590906 Nicotinamide Sigma Cat#N0636 Afamin/Wnt3a conditioned medium MBL lifescience Cat#J2-001 Activin A Nacalai Tesque Cat#18585-81 CHIR99021 Cayman Chemicals Cat#13122 Recombinant human FGF4 Peprotech Cat#100-31 Recombinant human BMP-4 R&D Cat# 314-BP Wnt-C59 Chemscene LLC Cat#CS-1420 PIK90 Cayman Chemicals Cat#10010749 Retinoic acid Sigma Aldrich Cat#R2625 Purmorphamine Tocris Bioscience Cat#4551 Y-27632 Fujifilm Wako Pure Chemical Cat#253-00513 FGF-basic(154a.a.), Human, Recombinant Peprotech Cat#100-18B Recombinant Human IGF-I BioLegend Cat#590906 Cellartis DEF-CSTM 500 culture system Takara Bio Cat#Y50101 Advanced DMEM/F12 Thermo Fisher Cat#12634010 DMEM, high glucose Thermo Fisher Cat#11965-092 RPMI 1640 with L-Gln, liquid Nacalai Tesque Cat#30264-85 Embryonic stem cell Fetal Bovine Serum Thermo Fisher Cat#16141079 GlutaMAXTM Supplement Thermo Fisher Cat#35050-061 HEPES buffer Nacalai Tesque Cat#17557-94 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#26253-84 Matrigel Growth Factor Reduced Corning Cat#356231 N-2 supplement (100X) Thermo Fisher Cat#17502-048 B-27 Supplement (50X), serum free Thermo Fisher Cat#17504-044 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat#12587010 TrypLE Express Enzyme Thermo Fisher Cat#12604013 Accumax Nacalai Tesque Cat#17087-54 Hoechst 33342 Thermo Fisher Cat#H3570 VECTASHIELD mounting medium with DAPI VECTOR Laboratories Cat#H1200 DAPI solution (1 mg/mL) Nacalai Tesque Cat#19178-91 The P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 Critical commercial assays RNAscope 2.5HD Assay-Red Advanced Cell Diagnostics Cat#322350 RNeasy Mini kit QIAGEN Cat#74106 QuantiTect Reverse Transcription Kit QIAGEN Cat#205313 Experimental models: Cell lines Human induced pluripotent stem cell line: HiPS-RIKEN-2F RIKEN BRC Cell Bank (Japan) HPS0014 Human induced pluripotent stem cell line: PB001 Kindly gifted by Dr Hideki Masaki (Institute of Medical Science, the University of Tokyo) N/A Human induced pluripotent stem cell line: TkdA3-4 Institute of Medical Science, the University of Tokyo N/A (Continued on next page) Cell Reports Methods 4, 100930, December 16, 2024 e2 Report ll OPEN ACCESS
Staining:Article Title: Direct analysis of hepatic stellate cells with flow cytometry in specimens derived from the human liver
Article Snippet: .. Finally, a DAPI solution (Nacalai, 1:1000) was added for nuclear staining. .. The cells were observed using BZ-X800 (KEYENCE).
Laser-Scanning Microscopy:
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