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4 6 diamidino 2 phenylindole dapi reagent  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology 4 6 diamidino 2 phenylindole dapi reagent
    4 6 Diamidino 2 Phenylindole Dapi Reagent, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+reagent/DAPI+Reagent/pmc12116517-87-6-10
    Average 94 stars, based on 27 article reviews
    4 6 diamidino 2 phenylindole dapi reagent - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Indole ring linked thiazolidone hybrid molecules as potent tubulin inhibitors
    Article Snippet: Alexa Fluor 488 anti-mouse secondary antibody (Abcam, ab150113) was applied to samples (1:200) for 45 min at 37 oC. .. Nuclear staining was done by DAPI (Elabscience, E-CK-A163) for 10 min at room temperature. .. After washing steps, cells were mounted by Fluoromount-G (Invitrogen, 00495802) and cell imaging was performed using Zeiss Axio Fluorescence Microscope.

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway
    Article Snippet: The primary antibody was recovered, and the wells were washed three times with PBST (Solarbio, Beijing, China, P1033) and incubated with the secondary antibody (Proteintech, Wuhan, China, B900210, 1:1000) for 1 h in the dark. .. The nuclei of the cells were stained with DAPI (Elabscience, Wuhan, China, E-CK-A163) for 5 min, and finally, a drop of anti-fluorescence quencher (Solarbio, Beijing, China, S2110) was added. ..

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway.
    Article Snippet: The primary antibody was recovered, and the wells were washed three times with PBST (Solarbio, Beijing, China, P1033) and incubated with the secondary antibody (Proteintech, Wuhan, China, B900210, 1:1000) for 1 h in the dark. .. The nuclei of the cells were stained with DAPI (Elabscience, Wuhan, China, E-CK-A163) for 5 min, and finally, a drop of anti-fluorescence quencher (Solarbio, Beijing, China, S2110) was added. ..

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway.
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).

    Incubation:

    Article Title: Dehydrocorybulbine blocks the Lyn-mediated MAPK pathway to promote anti-inflammatory polarization of microglia in mice after sevoflurane anesthesia.
    Article Snippet: Dehydrocorybulbine (DHCB) has demonstrated efficacy in alleviating thermally induced acute pain.. The present study sought to study the impact of DHCB on postoperative cognitive dysfunction (POCD).. Mice or mouse BV2 cells were exposed to sevoflurane for modeling.

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway.
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).

    Cell Characterization:

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway.
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).



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