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dapi reagent  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology dapi reagent
    Dapi Reagent, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+reagent/DAPI+Reagent/pm42869340-133-16-19
    Average 94 stars, based on 30 article reviews
    dapi reagent - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Inverse Vaccine Nano-Mimics: In Situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post Myocardial Infarction
    Article Snippet: .. For T-cell immunofluorescence labeling, nuclei were labeled with DAPI (E-CK-A163, Elabscience, China), CD4 with FITC (E-AB-F1097C, Elabscience, China), and CD127 with Alexa Fluor 555 (16-1271-85, eBioscience, USA). ..

    Labeling:

    Article Title: Inverse Vaccine Nano-Mimics: In Situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post Myocardial Infarction
    Article Snippet: .. For T-cell immunofluorescence labeling, nuclei were labeled with DAPI (E-CK-A163, Elabscience, China), CD4 with FITC (E-AB-F1097C, Elabscience, China), and CD127 with Alexa Fluor 555 (16-1271-85, eBioscience, USA). ..

    Staining:

    Article Title: Indole ring linked thiazolidone hybrid molecules as potent tubulin inhibitors
    Article Snippet: Alexa Fluor 488 anti-mouse secondary antibody (Abcam, ab150113) was applied to samples (1:200) for 45 min at 37 oC. .. Nuclear staining was done by DAPI (Elabscience, E-CK-A163) for 10 min at room temperature. .. After washing steps, cells were mounted by Fluoromount-G (Invitrogen, 00495802) and cell imaging was performed using Zeiss Axio Fluorescence Microscope.

    Article Title: Period3 transgenic mice attenuate cadmium-induced chronic renal toxicity associated with reduced Trpm7 expression
    Article Snippet: Tissue sections were also used for the TUNEL assay (One-Step TUNEL In Situ Apoptosis Kit; Elabscience, Houston, TX, USA), performed according to the manufacturer instructions, in which red fluorescent cells are considered apoptotic. .. 4′,6-Diamidino-2-phenylindole (DAPI; Elabscience) was used to stain the nuclei, which are visualized as blue fluorescence signals. ..

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway.
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).

    Article Title: WSSV355 enhances IDH1 enzymatic activity and promotes efficient WSSV replication.
    Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof 2 White spot syndrome virus (WSSV) drives extensive metabolic reprogramming to support viral replication, including activation of reductive carboxylation, a pathway in which host isocitrate dehydrogenase 1 (IDH1) has a central role.. However, the mechanisms by which viral factors regulate IDH1 and associated metabolic networks remain unclear.. Here, we identified WSSV355 as a key viral regulator that contributed to IDH1 binding and activation during infection.

    Incubation:

    Article Title: Dehydrocorybulbine blocks the Lyn-mediated MAPK pathway to promote anti-inflammatory polarization of microglia in mice after sevoflurane anesthesia.
    Article Snippet: Dehydrocorybulbine (DHCB) has demonstrated efficacy in alleviating thermally induced acute pain.. The present study sought to study the impact of DHCB on postoperative cognitive dysfunction (POCD).. Mice or mouse BV2 cells were exposed to sevoflurane for modeling.

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway.
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).

    Article Title: WSSV355 enhances IDH1 enzymatic activity and promotes efficient WSSV replication.
    Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof 2 White spot syndrome virus (WSSV) drives extensive metabolic reprogramming to support viral replication, including activation of reductive carboxylation, a pathway in which host isocitrate dehydrogenase 1 (IDH1) has a central role.. However, the mechanisms by which viral factors regulate IDH1 and associated metabolic networks remain unclear.. Here, we identified WSSV355 as a key viral regulator that contributed to IDH1 binding and activation during infection.

    Fluorescence:

    Article Title: Period3 transgenic mice attenuate cadmium-induced chronic renal toxicity associated with reduced Trpm7 expression
    Article Snippet: Tissue sections were also used for the TUNEL assay (One-Step TUNEL In Situ Apoptosis Kit; Elabscience, Houston, TX, USA), performed according to the manufacturer instructions, in which red fluorescent cells are considered apoptotic. .. 4′,6-Diamidino-2-phenylindole (DAPI; Elabscience) was used to stain the nuclei, which are visualized as blue fluorescence signals. ..

    Cell Characterization:

    Article Title: The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway.
    Article Snippet: .. Primary myoblast cells and the cells from the 3rd and 5th days of myogenic differentiation were fixed with 4% paraformaldehyde (Biosharp, Hefei, China, BL539A) for 15 min, permeabilized in 0.5% Triton X-100 (Beyotime, Shanghai, China, P0096) for 15 min, and blocked with 1% BSA (Solarbio, Beijing, China, SW3015) for 1 h. The primary antibody MyHC (Priteintech, Wuhan, China, 22281-1-AP, 1:500) was added and incubated overnight, followed by the addition of the corresponding secondary antibody, and incubated for 2 h. Subsequently, DAPI (Elabscience, Wuhan, China, E-CK-A163) was added to stain the nuclei for 5 min. .. Images were captured using a fluorescence microscope (Nikon, Tokyo, Japan).



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