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dapi nuclear dye  (Proteintech)


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    Structured Review

    Proteintech dapi nuclear dye
    Dapi Nuclear Dye, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+nuclear+dye+solution/Drosha+Antibody/ppr0540980-91-10-8
    Average 96 stars, based on 597 article reviews
    dapi nuclear dye - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Monochromatic light affects intestinal barrier function of chick via an anti-oxidation pathway involving melatonin and its receptor Mel1b.
    Article Snippet: .. Paraffin jejunal sections were incubated with primary antibodies at 4◦C overnight: proliferating cell nuclear antigen (PCNA, 1:300; Proteintech, Wuhan, China), IgA (1:300; Abcam, Cambridge, England), Mel 1a (1:200; Abmart, Shanghai, China), Mel 1b (1:300; Abcam, USA) and Mel 1c (1:100; Abmart, Shanghai, China). .. Then, the sections were rinsed with PBS (0.01 M, pH = 7.4) and incubated with biotinylated secondary antibodies (Goat-anti-Mouse/Goat-anti-Rabbit, 1:300, CWBio, Taizhou, China) and streptavidin-horseradish peroxidase (1:300, Vector Laboratories, Burlingame, CA, USA) successively for 2 h at room temperature.

    Article Title: Multi-omics reveals cholesterol-driven macrophage metabolic reprogramming and inflammation in chronic obstructive pulmonary disease.
    Article Snippet: .. The film was blocked by Protein-Free Rapid Blocking Solution (Servicebio®, Wuhan, China) and then incubated with primary antibodies including 3β-hydroxy-Δ5-C27steroid dehydrogenase (HSD3B7, Affinity Biosciences, OH, USA), peptidylprolyl isomerase A (PPIA, Proteintech, Wuhan, China), nuclear factor-κB p65 (P65, Proteintech, Wuhan, China), phosphorylatedNF-κB p65 (Proteintech, Wuhan, China) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Proteintech, Wuhan, China), overnight at 4◦C. ..

    Blocking Assay:

    Article Title: Multi-omics reveals cholesterol-driven macrophage metabolic reprogramming and inflammation in chronic obstructive pulmonary disease.
    Article Snippet: .. The film was blocked by Protein-Free Rapid Blocking Solution (Servicebio®, Wuhan, China) and then incubated with primary antibodies including 3β-hydroxy-Δ5-C27steroid dehydrogenase (HSD3B7, Affinity Biosciences, OH, USA), peptidylprolyl isomerase A (PPIA, Proteintech, Wuhan, China), nuclear factor-κB p65 (P65, Proteintech, Wuhan, China), phosphorylatedNF-κB p65 (Proteintech, Wuhan, China) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Proteintech, Wuhan, China), overnight at 4◦C. ..

    Multiple Displacement Amplification:

    Article Title: FGF4 alleviates the lung cell damage caused by high glucose via AMPK-PGC-1 signaling axis in vitro and in vivo
    Article Snippet: Cell culture plates, FBS, BSA and TBST buffer were from Beyotime Institute of Biotechnology. .. CyclinD1, proliferating cell nuclear antigen (PCNA), phosphorylated (p-)IκB, p-P65 and p-STAT3 were from Proteintech Group, Inc. malondialdehyde (MDA; cat. no. A003-1-2), SOD (cat. no. A001-3-2) and glutathione peroxidase (GPX; cat. no. H545-1-1) were from Nanjing Jiancheng Bioengineering Institute. .. MLE12 cells and BEAS-2B were cultured using Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.).

    Western Blot:

    Article Title: DNA repair pathway-related proteins are involved in the circularization step of microDNA eccDNA fib-L .
    Article Snippet: Subcellular distribution of Polθ in the cytoplasm and nucleus Cytoplasmic and nuclear proteins from BmN cells were extracted following the protocol provided by the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, China). .. Western blot was performed to verify the separation of cytoplasmic and nuclear fractions using primary antibodies against H3 (Proteintech, USA) and tubulin (Proteintech, USA) at a 1:1000 dilution, with HRP-labeled goat anti-mouse IgG (Proteintech, USA) at a 1:5000 dilution as the secondary antibody. ..

    Article Title: Therapeutic potential of oral edaravone in diabetic endothelial dysfunction through NF-κB and p38 pathway inhibition.
    Article Snippet: Objective: Diabetes mellitus is a chronic metabolic disorder frequently associated with endothelial dysfunction, a major contributor to cardiovascular complications.. This study aimed to investigate the protective effects of oral edaravone (OED) on endothelial dysfunction in diabetic models and explore its underlying molecular

    Expressing:

    Article Title: Therapeutic potential of oral edaravone in diabetic endothelial dysfunction through NF-κB and p38 pathway inhibition.
    Article Snippet: Objective: Diabetes mellitus is a chronic metabolic disorder frequently associated with endothelial dysfunction, a major contributor to cardiovascular complications.. This study aimed to investigate the protective effects of oral edaravone (OED) on endothelial dysfunction in diabetic models and explore its underlying molecular

    Control:

    Article Title: Therapeutic potential of oral edaravone in diabetic endothelial dysfunction through NF-κB and p38 pathway inhibition.
    Article Snippet: Objective: Diabetes mellitus is a chronic metabolic disorder frequently associated with endothelial dysfunction, a major contributor to cardiovascular complications.. This study aimed to investigate the protective effects of oral edaravone (OED) on endothelial dysfunction in diabetic models and explore its underlying molecular



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