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dapi nuclear dye fcmacs cat  (Cell Signaling Technology Inc)


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Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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  • 98

    Structured Review

    Cell Signaling Technology Inc dapi nuclear dye fcmacs cat
    Dapi Nuclear Dye Fcmacs Cat, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+nuclear+dye+solution/DAPI/pm39577415-270-193-199
    Average 98 stars, based on 2159 article reviews
    dapi nuclear dye fcmacs cat - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Staining:

    Article Title: Metabolic‐Redox Remodeling of Inflammatory Synovium by Mannose‐Modified Selenium Nanoparticles for Osteoarthritis Therapy
    Article Snippet: .. Cells were washed with PBS, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X‐100, and stained with Actin‐Tracker Green‐488 (1:200, Beyotime) and DAPI (1:1000, CST). .. Cellular internalization was observed via a Leica STELLARIS 5 confocal microscope and quantitatively assessed by flow cytometry (BD LSRFortessa) with DiD fluorescence measured in the APC channel.

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: .. Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature. ..

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: In brief, Adenoassociated virus (AAV) vector-mediated overexpression of mouse RUNX1, control (ZsGreen), Adenoassociated virus (AAV)-Cre-ZsGreen and Control (ZsGreen) were purchased from Hanbio biotechnology (Shanghai, China). .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature. .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature.

    Confocal:

    Article Title: Ondansetron reduces seizures and improves cognitive impairment in a rat model of temporal lobe epilepsy
    Article Snippet: After PBS washes, samples were incubated with Alexa Fluor-conjugated secondary antibodies (goat anti-mouse 555 and goat anti-rabbit 647, 1:1000, CST) for 1 hr at RT in the dark. .. Nuclei were counterstained with DAPI (CST) for 10 min. Confocal images were acquired at 60 × magnification. .. The rats were uninterruptedly recorded using a 4-camera video system (Hikvision, China) in transparent cages.

    Incubation:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: Images were acquired using a digital slide scanner (Axio Scan Z1, Carl Zeiss). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083).

    Blocking Assay:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: Images were acquired using a digital slide scanner (Axio Scan Z1, Carl Zeiss). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083).



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