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dapi fluorescent probe  (Beyotime)


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    Beyotime dapi fluorescent probe
    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
    Dapi Fluorescent Probe, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+fluorescent+probe/DAPI/pm40067175-280-24-30
    Average 99 stars, based on 32727 article reviews
    dapi fluorescent probe - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Orally Administered Functional Polyphenol-Nanozyme-Armored Probiotics for Enhanced Amelioration of Intestinal Inflammation and Microbiota Dysbiosis."

    Article Title: Orally Administered Functional Polyphenol-Nanozyme-Armored Probiotics for Enhanced Amelioration of Intestinal Inflammation and Microbiota Dysbiosis.

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    doi: 10.1002/advs.202411939

    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with DAPI (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
    Figure Legend Snippet: Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with DAPI (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.

    Techniques Used: Activity Assay, Staining, Enzyme-linked Immunosorbent Assay

    Related Articles

    Incubation:

    Article Title: Aged ZnO Nanoparticles Promote Breast Cancer Lung Metastasis by Remodeling the Pulmonary Microenvironment Through the CXCL10/Wnt/β‐Catenin Axis
    Article Snippet: NIH‐3T3 cells, RAW264.7 cells, or 4T1/MCF7 cells treated with 40 ng mL −1 rCXCL10 were seeded onto the coverslips and cultured for 72 h. Cells were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.2% Triton X‐100 (Solarbio, T8200) for 5 min, and blocked with 5% BSA (Solarbio, A8850) for 2 h. For tissue sections, slides underwent deparaffinization, rehydration, and blocking with 5% BSA for 2 h. Both coverslips and tissue sections were incubated overnight at 4°C with primary antibodies against α‐SMA (Affinity, AF1032), CD206 (Affinity, DF4149), or β‐Catenin (Proteintech, PRO‐CY‐50WY). .. After three washes, samples were incubated for 1 h at room temperature in the dark with Fluor488‐conjugated secondary antibody (Immunoway, RS32109), counterstained with DAPI (Beyotime, C1005) for 3 min, and mounted with antifade medium (Abbkine, BMU104). ..

    Lysis:

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: Mouse ELISA kits for quantifying IL‐6, IL‐1β, TNF‐α, and IFN‐β were supplied by Beijing Solarbio Science & Technology Co., Ltd. (China). .. DAPI, PMSF, and RIPA lysis buffer were acquired from Beyotime Biotechnology (China). .. Antibodies targeting phospho‐TBK1 (Ser172, #5483), phospho‐STING (Ser365, #72971), total TBK1 (#38066), and total STING (#13647) were purchased from Cell Signaling Technology (CST) via Beijing Chengzhi Kewei Biotechnology Co., Ltd. (China).

    Fluorescence:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Microscopy:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Staining:

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark. .. Finally, the fluorescence images of the stained cells were obtained using a confocal laser scanning microscope (Leica TCS SP8, Leica Microsystems, Wetzlar, Germany) and analyzed with ImageJ software.



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    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
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    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
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    Image Search Results


    (A) WB protein bands and their normalized integrated optical density for (B) the cortex and (C) hippocampus areas. The representative fluorescence microscopy images of the brain tissue (D) before and (E) after 808 nm tPBMT, stained with Arginase1 antibody (green), iNOS antibody (red), and DAPI (blue); and (F) the relative fluorescence intensities of the antibodies. The data are presented as M ± SD ( N = 3), * P < 0.05, ** P < 0.01 for data with a statistically significant difference.

    Journal: PLOS One

    Article Title: Transcranial photobiomodulation therapy with 808 nm light changes expression of genes and proteins associated with neuroprotection, neuroinflammation, oxidative stress, and Alzheimer’s disease: Whole RNA sequencing of mouse cortex and hippocampus

    doi: 10.1371/journal.pone.0326881

    Figure Lengend Snippet: (A) WB protein bands and their normalized integrated optical density for (B) the cortex and (C) hippocampus areas. The representative fluorescence microscopy images of the brain tissue (D) before and (E) after 808 nm tPBMT, stained with Arginase1 antibody (green), iNOS antibody (red), and DAPI (blue); and (F) the relative fluorescence intensities of the antibodies. The data are presented as M ± SD ( N = 3), * P < 0.05, ** P < 0.01 for data with a statistically significant difference.

    Article Snippet: The tissue slices were stained with Arginase 1 antibody, inducible nitric oxide synthase (iNOS) antibody [ ], and DAPI fluorescent probe (Thermo Fisher Scientific, USA).

    Techniques: Fluorescence, Microscopy, Staining

    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with DAPI (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Orally Administered Functional Polyphenol-Nanozyme-Armored Probiotics for Enhanced Amelioration of Intestinal Inflammation and Microbiota Dysbiosis.

    doi: 10.1002/advs.202411939

    Figure Lengend Snippet: Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with DAPI (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.

    Article Snippet: 3,3′,5,5′-Tetramethylbenzidine (TMB: ST746), DCFH-DA fluorescent probe, Total SOD Assay Kit with WST-8 (Cat# S0101S), Catalase Assay Kit (Cat# S0051), Hoechst 33342 (Cat# C1028), and DAPI fluorescent probe were acquired from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Techniques: Activity Assay, Staining, Enzyme-linked Immunosorbent Assay