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Merck KGaA 4′,6-diamidino-2-phenylindole d9542
4′,6 Diamidino 2 Phenylindole D9542, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+d9542/dapi/pm39622370-89-8-11
Average 90 stars, based on 1 article reviews
4′,6-diamidino-2-phenylindole d9542 - by Bioz Stars, 2026-10
90/100 stars

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Staining:

Article Title: Prenatal Delta-9-Tetrahydrocannabinol Exposure Induces Transcriptional Alterations in Dopaminergic System with Associated Electrophysiological Dysregulation in the Prefrontal Cortex of Adolescent Rats.
Article Snippet: Thereafter, sections were incubated at 4 ◦C with either the rabbit polyclonal primary antibody antityrosine hydroxylase (TH, 1:1000, Merck, Darmstadt, Germany, #AB152, 48 h) or rabbit polyclonal primary antibody anti-Netrin-1 (1:600, Alomone Labs, Jerusalem, Israel, #ANR121, 96 h), rinsed three times in 0.1 M PB, and then incubated with the secondary antibody, Atto 488-labeled goat anti-rabbit IgG (1:400, Merck, Germany, #18772) in 0.1 M PB at room temperature (3 h).

Article Title: GDF15 modulates aging-associated adaptions in the mechanoresponse of periodontal ligament fibroblasts.
Article Snippet: Cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000 in PBS) and coverslips were embedded with Mowiol®4-88 (Merck Millipore, Burlington, MA, USA) for imaging. β-Galactosidase staining CellEventTM Senescence Green Detection Kit (Thermo Fisher Scientific) was used to detect cellular senescence in hPdLFs fixed with 4% PFA in PBS.

Article Title: Prenatal Delta-9-Tetrahydrocannabinol Exposure Induces Transcriptional Alterations in Dopaminergic System with Associated Electrophysiological Dysregulation in the Prefrontal Cortex of Adolescent Rats
Article Snippet: Afterward, sections were incubated for 10 min in 4′,6-diamidino-2-phenylindole (DAPI; 1:10,000, Merck, Italy, D9542) to allow the visualization of cell nuclei, rinsed in PB 0.1 M, and mounted onto super-frost glass slides using Mowiol ® mounting medium.

Article Title: HOPS-dependent vesicle tethering lock inhibits endolysosomal fusions and autophagosome secretion upon the loss of Syntaxin17
Article Snippet: 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) , Merck KGaA , D8417.

Imaging:

Article Title: Prenatal Delta-9-Tetrahydrocannabinol Exposure Induces Transcriptional Alterations in Dopaminergic System with Associated Electrophysiological Dysregulation in the Prefrontal Cortex of Adolescent Rats.
Article Snippet: Thereafter, sections were incubated at 4 ◦C with either the rabbit polyclonal primary antibody antityrosine hydroxylase (TH, 1:1000, Merck, Darmstadt, Germany, #AB152, 48 h) or rabbit polyclonal primary antibody anti-Netrin-1 (1:600, Alomone Labs, Jerusalem, Israel, #ANR121, 96 h), rinsed three times in 0.1 M PB, and then incubated with the secondary antibody, Atto 488-labeled goat anti-rabbit IgG (1:400, Merck, Germany, #18772) in 0.1 M PB at room temperature (3 h).

Article Title: GDF15 modulates aging-associated adaptions in the mechanoresponse of periodontal ligament fibroblasts.
Article Snippet: Cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000 in PBS) and coverslips were embedded with Mowiol®4-88 (Merck Millipore, Burlington, MA, USA) for imaging. β-Galactosidase staining CellEventTM Senescence Green Detection Kit (Thermo Fisher Scientific) was used to detect cellular senescence in hPdLFs fixed with 4% PFA in PBS.

Article Title: Prenatal Delta-9-Tetrahydrocannabinol Exposure Induces Transcriptional Alterations in Dopaminergic System with Associated Electrophysiological Dysregulation in the Prefrontal Cortex of Adolescent Rats
Article Snippet: Afterward, sections were incubated for 10 min in 4′,6-diamidino-2-phenylindole (DAPI; 1:10,000, Merck, Italy, D9542) to allow the visualization of cell nuclei, rinsed in PB 0.1 M, and mounted onto super-frost glass slides using Mowiol ® mounting medium.

Article Title: HOPS-dependent vesicle tethering lock inhibits endolysosomal fusions and autophagosome secretion upon the loss of Syntaxin17
Article Snippet: 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) , Merck KGaA , D8417.



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