dapi #d1306 (Thermo Fisher)
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dapi #d1306
Dapi #D1306, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+d1306/dapi/pm40576576-72-5-7
Average 90 stars, based on 1 article reviews
Dapi #D1306, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+d1306/dapi/pm40576576-72-5-7
Average 90 stars, based on 1 article reviews
dapi #d1306 - by Bioz Stars,
2026-09
90/100 stars
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Immunocytochemistry:Article Title: Neuron-derived extracellular vesicles in plasma present a potential non-invasive biomarker for Huntingtin protein and RNA assessment in Huntington disease Article Snippet: .. NSCs cultures were characterized by ICC using the markers Nestin 10C2 (StemCell, 60091.1, 1:1000), Sox1 JJ20-40 (Thermo Scientific, MA532447, 1:1000) and Pax6 Poly19013 (BioLegend, 901301, 1:1000), and absence of the pluripotency marker Oct4 3A2A20 (StemCell, 60093.1, 1:1000) in the two lines. iPSC-differentiated neurons were stained for DARPP-32 19A3 (Cell signaling, 2306S, 1:1000), NeuN clone A60 (Millipore, mab377), β3-Tubulin D71G9 (Cell signaling, 5568S, 1:1000), MAP2 (Abcam, ab32454, 1:1000) and counterstained with Marker:Article Title: Neuron-derived extracellular vesicles in plasma present a potential non-invasive biomarker for Huntingtin protein and RNA assessment in Huntington disease Article Snippet: .. NSCs cultures were characterized by ICC using the markers Nestin 10C2 (StemCell, 60091.1, 1:1000), Sox1 JJ20-40 (Thermo Scientific, MA532447, 1:1000) and Pax6 Poly19013 (BioLegend, 901301, 1:1000), and absence of the pluripotency marker Oct4 3A2A20 (StemCell, 60093.1, 1:1000) in the two lines. iPSC-differentiated neurons were stained for DARPP-32 19A3 (Cell signaling, 2306S, 1:1000), NeuN clone A60 (Millipore, mab377), β3-Tubulin D71G9 (Cell signaling, 5568S, 1:1000), MAP2 (Abcam, ab32454, 1:1000) and counterstained with Staining:Article Title: Neuron-derived extracellular vesicles in plasma present a potential non-invasive biomarker for Huntingtin protein and RNA assessment in Huntington disease Article Snippet: .. NSCs cultures were characterized by ICC using the markers Nestin 10C2 (StemCell, 60091.1, 1:1000), Sox1 JJ20-40 (Thermo Scientific, MA532447, 1:1000) and Pax6 Poly19013 (BioLegend, 901301, 1:1000), and absence of the pluripotency marker Oct4 3A2A20 (StemCell, 60093.1, 1:1000) in the two lines. iPSC-differentiated neurons were stained for DARPP-32 19A3 (Cell signaling, 2306S, 1:1000), NeuN clone A60 (Millipore, mab377), β3-Tubulin D71G9 (Cell signaling, 5568S, 1:1000), MAP2 (Abcam, ab32454, 1:1000) and counterstained with Article Title: Microwave-assisted immunostaining for rapid labeling of matrix-embedded multicellular structures Article Snippet: A solution containing Alexa Fluor 488-conjugated anti-Lamin B1 (1:25; ab194106, Abcam, Eugene, OR), Alexa Fluor 555-conjugated anti-GM130 (1:10; Catalog #560066, BD Biosciences, Franklin Lakes, NJ), and Alexa Fluor 647-conjugated anti-β-Catenin (L54E2) (1:10; Catalog #4627, Cell Signaling Technology, Danvers, MA) in blocking buffer was added to the samples and microwaved at the indicated times. .. Nuclear staining was performed with a large volume of Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair Article Snippet: .. The cytoskeleton and nuclei were stained with rhodamine (Solarbio, China) and Article Title: Palmitic acid reduces LDLR-dependent uptake of macrophage-derived extracellular vesicles by hepatoma cells Article Snippet: .. Nuclei were stained with Blocking Assay:Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair Article Snippet: .. The cells were then blocked with normal goat serum at room temperature for 30 min. After blocking, the cells were incubated with primary antibodies specific for COL-1 (Proteintech, USA) for BMSCs and VEGF (Proteintech, USA) for HUVECs at 4 °C for 8–16 h. Following three washes with PBS, the cells were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA) in the dark for 1 h. The nuclei were counterstained with Incubation:Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair Article Snippet: .. The cells were then blocked with normal goat serum at room temperature for 30 min. After blocking, the cells were incubated with primary antibodies specific for COL-1 (Proteintech, USA) for BMSCs and VEGF (Proteintech, USA) for HUVECs at 4 °C for 8–16 h. Following three washes with PBS, the cells were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA) in the dark for 1 h. The nuclei were counterstained with Article Title: MitoQ Protects Against Oxidative Stress-Induced Mitochondrial Dysregulation in Human Cardiomyocytes Article Snippet: Cardiomyocytes were then incubated with Protein block (Dako, Victoria, Australia) to prevent unspecific antibody binding followed by Cardiac Troponin-T (cTnT 1:500; mouse polyclonal antibody; Cat# ab45932, Abcam) and Anti-Hsp60 (1:300; rabbit polyclonal antibody; Cat# ab46798, Abcam) overnight incubation at 4 °C. .. Cardiomyocytes were then incubated with Alexa Fluor 594 (1:200; goat anti-mouse secondary antibody; Cat# A20004, Thermo Fisher), Alexa Fluor 488 (1:200; goat anti-rabbit secondary antibody; Cat# A20000, Thermo Fisher) and Fluorescence:Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair Article Snippet: .. The cells were then blocked with normal goat serum at room temperature for 30 min. After blocking, the cells were incubated with primary antibodies specific for COL-1 (Proteintech, USA) for BMSCs and VEGF (Proteintech, USA) for HUVECs at 4 °C for 8–16 h. Following three washes with PBS, the cells were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA) in the dark for 1 h. The nuclei were counterstained with Microscopy:Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair Article Snippet: .. The cells were then blocked with normal goat serum at room temperature for 30 min. After blocking, the cells were incubated with primary antibodies specific for COL-1 (Proteintech, USA) for BMSCs and VEGF (Proteintech, USA) for HUVECs at 4 °C for 8–16 h. Following three washes with PBS, the cells were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA) in the dark for 1 h. The nuclei were counterstained with Article Title: Palmitic acid reduces LDLR-dependent uptake of macrophage-derived extracellular vesicles by hepatoma cells Article Snippet: .. Nuclei were stained with other:Article Title: Divergent Cell-Type Specific Hypoxia Responses in Human Stem Cell–Derived and Primary Islets Article Snippet: Enzyme-linked Immunosorbent Assay:Article Title: HydroWrap for T2DM-Related Fractures: A smart H 2 S-delivery controller modulating Macrophage senescence Article Snippet: Antibodies targeting β-actin, p65, p-p65, Parkin, p62, and LC3B, along with goat anti-rabbit IgG-HRP and goat anti-mouse IgG-HRP, were obtained from Cell Signaling Technology (CST, USA) and GeneTex (USA). .. MitoTracker, phalloidin, and |
