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dapi (1:5000) counterstaining  (Merck KGaA)

 
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    Merck KGaA dapi (1:5000) counterstaining
    Dapi (1:5000) Counterstaining, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+counterstaining/dapi++1+5000++counterstaining/pmc07821154-157-32-37
    Average 90 stars, based on 1 article reviews
    dapi (1:5000) counterstaining - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: The Impact of Curcumin on the Inflammatory Profile of SW982 Cells in a Rheumatoid Arthritis Model
    Article Snippet: Afterwards, primary antibodies were rinsed with PBS for 4 × 5 min and donkey anti-mouse secondary antibodies conjugated with DyLight-488 (Novus Biologicals, USA) in 1 : 500 dilution (in blocking serum) were added for 2-hour incubation at room temperature and no light. .. After the incubation, cells were rinsed with PBS for 4 × 5 min and DAPI (dilution 1 : 1000, Merck KGaA, Germany) counterstaining was performed for 10 min at room temperature. .. Finally, cells were rinsed with PBS for 3 × 5 min and the culture plate was imaged on a Zeiss Cell Observer SD confocal microscope (Carl Zeiss, Germany) using a 10x air objective (NA 0.30) and QImaging Rolera EM-C2 EMCCD camera.

    Article Title: Intranasal mesenchymal stem cell therapy to boost myelination after encephalopathy of prematurity
    Article Snippet: .. The following day, sections were washed in PBS and incubated with alexafluor‐594 and ‐488 conjugated secondary antibodies (Life technologies, Carlsbad, CA; 1:200–500) for 1–2 hr at room temperature, followed by DAPI (1:5000) counterstaining and embedment in Fluorsave (Merck Millipore, 345789). ..

    Fluorsave:

    Article Title: Intranasal mesenchymal stem cell therapy to boost myelination after encephalopathy of prematurity
    Article Snippet: .. The following day, sections were washed in PBS and incubated with alexafluor‐594 and ‐488 conjugated secondary antibodies (Life technologies, Carlsbad, CA; 1:200–500) for 1–2 hr at room temperature, followed by DAPI (1:5000) counterstaining and embedment in Fluorsave (Merck Millipore, 345789). ..



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    Image Search Results


    (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Journal: Bioengineering & Translational Medicine

    Article Title: Extracellular matrix microarchitecture modulates cellular behavior and extracellular vesicle phenotypes in biomimetic tendon models

    doi: 10.1002/btm2.70134

    Figure Lengend Snippet: (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Article Snippet: Cellular morphology was assessed via actin staining (Invitrogen, Cat. No. A12379) and nuclear counterstaining with DAPI (Fisher Scientific, Cat. No. H‐1200‐10) ( n = 3).

    Techniques: Staining, Derivative Assay, Control