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zero-pixel shift filter sets: dapi-5060c-zero, fitc-5050-000, cy3-4040c-zero, cy5-4040c-zero  (IDEX)

 
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    Structured Review

    IDEX zero-pixel shift filter sets: dapi-5060c-zero, fitc-5050-000, cy3-4040c-zero, cy5-4040c-zero

    Zero Pixel Shift Filter Sets: Dapi 5060c Zero, Fitc 5050 000, Cy3 4040c Zero, Cy5 4040c Zero, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+(dapi-5060c-zero)/cy5+4040c/pmc07186551-3-94-100
    Average 90 stars, based on 1 article reviews
    zero-pixel shift filter sets: dapi-5060c-zero, fitc-5050-000, cy3-4040c-zero, cy5-4040c-zero - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification"

    Article Title: Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification

    Journal: Data in Brief

    doi: 10.1016/j.dib.2020.105511


    Figure Legend Snippet:

    Techniques Used: Expressing, Imaging, Fluorescence, Microscopy, Software, Staining, Immunofluorescence

    Related Articles

    Inverted Microscopy:

    Article Title: Visualizing highly bright and uniform cellular ultrastructure by expansion-microscopy with tetrahedral DNA nanostructures.
    Article Snippet: Expansion Microscopy (ExM) is a widely used super-resolution technique that enables imaging of structures beyond the diffraction limit of light.. However, ExM suffers from weak labeling signals and expansion distortions, limiting its applicability.. Here, we present an innovative approach called Tetrahedral DNA nanostructure Expansion Microscopy (TDN-ExM), addressing these limitations by using tetrahedral DNA nanostructures (TDNs) for fluorescence labeling.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647-labelld BRICHOS.

    Article Title: Rapid water flow navigates long-distance migration of thermophilic bacteria
    Article Snippet: 1.45; Olympus), a filter set (Cy3-4040C, Semrock), the CMOS camera, and the optical table.

    Article Title: Methods for diagnosing neoplastic lesions
    Article Snippet: The following filter cubes (Semrock, Rochester, N.Y.) were used: DAPI, DAPI-11LP-A-000; Alexa Fluor 488, GFP-1828A-NTE-ZERO; Alexa Fluor 546, FITC/TXRED-2X-B-NTE; Atto 647N or CF 633, Cy5-4040C-000.

    Article Title: The last resort antibiotic daptomycin exhibits two independent antibacterial mechanisms of action
    Article Snippet: Fluorescence microscopy of B. subtilis was performed using a Nikon Eclipse Ti equipped with a Nikon Plan Apo 100×/1.40 Oil Ph3 objective, CoolLED pE-4000 light source, Photometrics BSI sCMOS camera, and the filter sets: Chroma 49000 (EX350/50, DM400lp, EM460/50) used for daptomycin, Chroma 49002 (EX 470/40, DM495lpxr, EM 525/50) used for SYTOX Green and BODIPY FL-vancomycin, Chroma 49008 (EX560/40, DM585lprx, EM630/75) used for FM 5-95, and Nile red, and Semrock Cy5-4040C (EX 628/40, DM660lp, EM 692/40) used for DiSC 3 (5); while S. aureus samples were imaged using a Nikon Eclipse Ti2 equipped with a Photometrics PRIME BSI EXP camera, Lumencor Sola SE II FISH 365 light source and CFI Plan Apochromat DM Lambda 100X Oil objective (N.A.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway.
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647labelld BRICHOS.

    Fluorescence:

    Article Title: Visualizing highly bright and uniform cellular ultrastructure by expansion-microscopy with tetrahedral DNA nanostructures.
    Article Snippet: Expansion Microscopy (ExM) is a widely used super-resolution technique that enables imaging of structures beyond the diffraction limit of light.. However, ExM suffers from weak labeling signals and expansion distortions, limiting its applicability.. Here, we present an innovative approach called Tetrahedral DNA nanostructure Expansion Microscopy (TDN-ExM), addressing these limitations by using tetrahedral DNA nanostructures (TDNs) for fluorescence labeling.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647-labelld BRICHOS.

    Article Title: Rapid water flow navigates long-distance migration of thermophilic bacteria
    Article Snippet: 1.45; Olympus), a filter set (Cy3-4040C, Semrock), the CMOS camera, and the optical table.

    Article Title: Methods for diagnosing neoplastic lesions
    Article Snippet: The following filter cubes (Semrock, Rochester, N.Y.) were used: DAPI, DAPI-11LP-A-000; Alexa Fluor 488, GFP-1828A-NTE-ZERO; Alexa Fluor 546, FITC/TXRED-2X-B-NTE; Atto 647N or CF 633, Cy5-4040C-000.

    Article Title: The last resort antibiotic daptomycin exhibits two independent antibacterial mechanisms of action
    Article Snippet: Fluorescence microscopy of B. subtilis was performed using a Nikon Eclipse Ti equipped with a Nikon Plan Apo 100×/1.40 Oil Ph3 objective, CoolLED pE-4000 light source, Photometrics BSI sCMOS camera, and the filter sets: Chroma 49000 (EX350/50, DM400lp, EM460/50) used for daptomycin, Chroma 49002 (EX 470/40, DM495lpxr, EM 525/50) used for SYTOX Green and BODIPY FL-vancomycin, Chroma 49008 (EX560/40, DM585lprx, EM630/75) used for FM 5-95, and Nile red, and Semrock Cy5-4040C (EX 628/40, DM660lp, EM 692/40) used for DiSC 3 (5); while S. aureus samples were imaged using a Nikon Eclipse Ti2 equipped with a Photometrics PRIME BSI EXP camera, Lumencor Sola SE II FISH 365 light source and CFI Plan Apochromat DM Lambda 100X Oil objective (N.A.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway.
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647labelld BRICHOS.

    Microscopy:

    Article Title: Visualizing highly bright and uniform cellular ultrastructure by expansion-microscopy with tetrahedral DNA nanostructures.
    Article Snippet: Expansion Microscopy (ExM) is a widely used super-resolution technique that enables imaging of structures beyond the diffraction limit of light.. However, ExM suffers from weak labeling signals and expansion distortions, limiting its applicability.. Here, we present an innovative approach called Tetrahedral DNA nanostructure Expansion Microscopy (TDN-ExM), addressing these limitations by using tetrahedral DNA nanostructures (TDNs) for fluorescence labeling.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647-labelld BRICHOS.

    Article Title: Rapid water flow navigates long-distance migration of thermophilic bacteria
    Article Snippet: 1.45; Olympus), a filter set (Cy3-4040C, Semrock), the CMOS camera, and the optical table.

    Article Title: Methods for diagnosing neoplastic lesions
    Article Snippet: The following filter cubes (Semrock, Rochester, N.Y.) were used: DAPI, DAPI-11LP-A-000; Alexa Fluor 488, GFP-1828A-NTE-ZERO; Alexa Fluor 546, FITC/TXRED-2X-B-NTE; Atto 647N or CF 633, Cy5-4040C-000.

    Article Title: The last resort antibiotic daptomycin exhibits two independent antibacterial mechanisms of action
    Article Snippet: Fluorescence microscopy of B. subtilis was performed using a Nikon Eclipse Ti equipped with a Nikon Plan Apo 100×/1.40 Oil Ph3 objective, CoolLED pE-4000 light source, Photometrics BSI sCMOS camera, and the filter sets: Chroma 49000 (EX350/50, DM400lp, EM460/50) used for daptomycin, Chroma 49002 (EX 470/40, DM495lpxr, EM 525/50) used for SYTOX Green and BODIPY FL-vancomycin, Chroma 49008 (EX560/40, DM585lprx, EM630/75) used for FM 5-95, and Nile red, and Semrock Cy5-4040C (EX 628/40, DM660lp, EM 692/40) used for DiSC 3 (5); while S. aureus samples were imaged using a Nikon Eclipse Ti2 equipped with a Photometrics PRIME BSI EXP camera, Lumencor Sola SE II FISH 365 light source and CFI Plan Apochromat DM Lambda 100X Oil objective (N.A.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway.
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647labelld BRICHOS.

    Fluorescence In Situ Hybridization:

    Article Title: Visualizing highly bright and uniform cellular ultrastructure by expansion-microscopy with tetrahedral DNA nanostructures.
    Article Snippet: Expansion Microscopy (ExM) is a widely used super-resolution technique that enables imaging of structures beyond the diffraction limit of light.. However, ExM suffers from weak labeling signals and expansion distortions, limiting its applicability.. Here, we present an innovative approach called Tetrahedral DNA nanostructure Expansion Microscopy (TDN-ExM), addressing these limitations by using tetrahedral DNA nanostructures (TDNs) for fluorescence labeling.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647-labelld BRICHOS.

    Article Title: Rapid water flow navigates long-distance migration of thermophilic bacteria
    Article Snippet: 1.45; Olympus), a filter set (Cy3-4040C, Semrock), the CMOS camera, and the optical table.

    Article Title: Methods for diagnosing neoplastic lesions
    Article Snippet: The following filter cubes (Semrock, Rochester, N.Y.) were used: DAPI, DAPI-11LP-A-000; Alexa Fluor 488, GFP-1828A-NTE-ZERO; Alexa Fluor 546, FITC/TXRED-2X-B-NTE; Atto 647N or CF 633, Cy5-4040C-000.

    Article Title: The last resort antibiotic daptomycin exhibits two independent antibacterial mechanisms of action
    Article Snippet: Fluorescence microscopy of B. subtilis was performed using a Nikon Eclipse Ti equipped with a Nikon Plan Apo 100×/1.40 Oil Ph3 objective, CoolLED pE-4000 light source, Photometrics BSI sCMOS camera, and the filter sets: Chroma 49000 (EX350/50, DM400lp, EM460/50) used for daptomycin, Chroma 49002 (EX 470/40, DM495lpxr, EM 525/50) used for SYTOX Green and BODIPY FL-vancomycin, Chroma 49008 (EX560/40, DM585lprx, EM630/75) used for FM 5-95, and Nile red, and Semrock Cy5-4040C (EX 628/40, DM660lp, EM 692/40) used for DiSC 3 (5); while S. aureus samples were imaged using a Nikon Eclipse Ti2 equipped with a Photometrics PRIME BSI EXP camera, Lumencor Sola SE II FISH 365 light source and CFI Plan Apochromat DM Lambda 100X Oil objective (N.A.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway.
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647labelld BRICHOS.

    Imaging:

    Article Title: Visualizing highly bright and uniform cellular ultrastructure by expansion-microscopy with tetrahedral DNA nanostructures.
    Article Snippet: Expansion Microscopy (ExM) is a widely used super-resolution technique that enables imaging of structures beyond the diffraction limit of light.. However, ExM suffers from weak labeling signals and expansion distortions, limiting its applicability.. Here, we present an innovative approach called Tetrahedral DNA nanostructure Expansion Microscopy (TDN-ExM), addressing these limitations by using tetrahedral DNA nanostructures (TDNs) for fluorescence labeling.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647-labelld BRICHOS.

    Article Title: Rapid water flow navigates long-distance migration of thermophilic bacteria
    Article Snippet: 1.45; Olympus), a filter set (Cy3-4040C, Semrock), the CMOS camera, and the optical table.

    Article Title: Methods for diagnosing neoplastic lesions
    Article Snippet: The following filter cubes (Semrock, Rochester, N.Y.) were used: DAPI, DAPI-11LP-A-000; Alexa Fluor 488, GFP-1828A-NTE-ZERO; Alexa Fluor 546, FITC/TXRED-2X-B-NTE; Atto 647N or CF 633, Cy5-4040C-000.

    Article Title: The last resort antibiotic daptomycin exhibits two independent antibacterial mechanisms of action
    Article Snippet: Fluorescence microscopy of B. subtilis was performed using a Nikon Eclipse Ti equipped with a Nikon Plan Apo 100×/1.40 Oil Ph3 objective, CoolLED pE-4000 light source, Photometrics BSI sCMOS camera, and the filter sets: Chroma 49000 (EX350/50, DM400lp, EM460/50) used for daptomycin, Chroma 49002 (EX 470/40, DM495lpxr, EM 525/50) used for SYTOX Green and BODIPY FL-vancomycin, Chroma 49008 (EX560/40, DM585lprx, EM630/75) used for FM 5-95, and Nile red, and Semrock Cy5-4040C (EX 628/40, DM660lp, EM 692/40) used for DiSC 3 (5); while S. aureus samples were imaged using a Nikon Eclipse Ti2 equipped with a Photometrics PRIME BSI EXP camera, Lumencor Sola SE II FISH 365 light source and CFI Plan Apochromat DM Lambda 100X Oil objective (N.A.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway.
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647labelld BRICHOS.

    Inverted Epifluorescence:

    Article Title: Visualizing highly bright and uniform cellular ultrastructure by expansion-microscopy with tetrahedral DNA nanostructures.
    Article Snippet: Expansion Microscopy (ExM) is a widely used super-resolution technique that enables imaging of structures beyond the diffraction limit of light.. However, ExM suffers from weak labeling signals and expansion distortions, limiting its applicability.. Here, we present an innovative approach called Tetrahedral DNA nanostructure Expansion Microscopy (TDN-ExM), addressing these limitations by using tetrahedral DNA nanostructures (TDNs) for fluorescence labeling.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647-labelld BRICHOS.

    Article Title: Rapid water flow navigates long-distance migration of thermophilic bacteria
    Article Snippet: 1.45; Olympus), a filter set (Cy3-4040C, Semrock), the CMOS camera, and the optical table.

    Article Title: Methods for diagnosing neoplastic lesions
    Article Snippet: The following filter cubes (Semrock, Rochester, N.Y.) were used: DAPI, DAPI-11LP-A-000; Alexa Fluor 488, GFP-1828A-NTE-ZERO; Alexa Fluor 546, FITC/TXRED-2X-B-NTE; Atto 647N or CF 633, Cy5-4040C-000.

    Article Title: The last resort antibiotic daptomycin exhibits two independent antibacterial mechanisms of action
    Article Snippet: Fluorescence microscopy of B. subtilis was performed using a Nikon Eclipse Ti equipped with a Nikon Plan Apo 100×/1.40 Oil Ph3 objective, CoolLED pE-4000 light source, Photometrics BSI sCMOS camera, and the filter sets: Chroma 49000 (EX350/50, DM400lp, EM460/50) used for daptomycin, Chroma 49002 (EX 470/40, DM495lpxr, EM 525/50) used for SYTOX Green and BODIPY FL-vancomycin, Chroma 49008 (EX560/40, DM585lprx, EM630/75) used for FM 5-95, and Nile red, and Semrock Cy5-4040C (EX 628/40, DM660lp, EM 692/40) used for DiSC 3 (5); while S. aureus samples were imaged using a Nikon Eclipse Ti2 equipped with a Photometrics PRIME BSI EXP camera, Lumencor Sola SE II FISH 365 light source and CFI Plan Apochromat DM Lambda 100X Oil objective (N.A.

    Article Title: The inhibitory action of the chaperone BRICHOS against the α-Synuclein secondary nucleation pathway.
    Article Snippet: Imaging was performed using a custom-built inverted epifluorescence microscope equipped with a charge-coupled-device (CCD) camera (Prime 95B, Photometrics, Tucson, AZ, USA) and bright-field LED light sources (Thorlabs, Newton, NJ, USA), using the Cy5-4040C-000 Filter set from Semrock (Laser 2000, Huntingdon, UK) for detection of Alexa647labelld BRICHOS.



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    Image Search Results


    Journal: Data in Brief

    Article Title: Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification

    doi: 10.1016/j.dib.2020.105511

    Figure Lengend Snippet:

    Article Snippet: How data were acquired , Data were acquired using a wide-field fluorescence microscope coupled to a CCD camera, a motorized stage driven by ultrasonic piezo technology for precise Z-positioning and a 120 W mercury short arc lamp as fluorescence illumination system. Model and make of the instruments used: Olympus BX-63 epifluorescence microscope equipped with Ultrasonic stage and UPlanApo 100 ×, 1.35NA oil-immersion objective (Olympus). An X-Cite 120 PC Lamp (EXFO), an ORCA-R2 Digital Interline CCD Camera (C10600-10B; Hamamatsu; 6.45 µm-pixel size) mounted using U-CMT and 1X-TVAD Olympus c-Mount Adapters, and zero-pixel shift filter sets: DAPI-5060C-Zero, FITC-5050-000, Cy3-4040C-Zero, and Cy5-4040C-Zero from Semrock. Software used for instrument control and image acquisition: MetaMorph (Molecular Devices)..

    Techniques: Expressing, Imaging, Fluorescence, Microscopy, Software, Staining, Immunofluorescence