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dab staining kit  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science dab staining kit
    Dab Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dab+staining+kit/dab+staining+kit/pm39747628-302-6-9
    Average 90 stars, based on 1 article reviews
    dab staining kit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: OsbHLH062 negatively regulates Fe homeostasis by enhancing OsHRZ1 targeting OsPRIs for degradation.
    Article Snippet: Iron (Fe) is crucial for plant growth and development, and many transcription factors are involved in the maintenance of Fe homeostasis.. In rice Fe signaling, the basic helix–loop–helix (bHLH) IVc proteins (OsPRI1/OsbHLH060, OsPRI2/OsbHLH058, OsPRI3/OsbHLH059, and OsPRI4/OsbHLH057) act as positive regulators, while Oryza sativa IRON-RELATED BHLH TRANSCRIPTION FACTOR 3 (OsIRO3/OsbHLH063) functions as a negative regulator.. Additionally, HEMERYTHRIN MOTIF-CONTAINING REALLY INTERESTING NEW GENE AND ZINC-FINGER PROTEIN1 (OsHRZ1) plays a role in targeting OsPRIs for degradation.

    Article Title: Alleviation of Gypenosides on Peripheral and Central Fatigue via Anti‐Inflammation, Anti‐Oxidation and Neurotransmitter Regulation
    Article Snippet: The AG RNAex Pro Reagent was provided by Hunan Agbio Biotechnology Co. Ltd. (Changsha, China). .. DAB staining kits and goat serum were supplied by Beijing Solarbio Science & Technology Co. Ltd. (Beijing, China). .. Primers of tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6) and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) used for quantitative real‐time polymerase chain reaction (qRT‐PCR) were commercially ordered from Shanghai Sangon Biotechnology Co. Ltd. (Shanghai, China), as demonstrated in Table .

    Article Title: Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice.
    Article Snippet: .. The staining was performed using a DAB Staining Kit (Solarbio, Beijing, China). ..

    Article Title: Helicobacter pylori infection induces STAT3/MYBL2/NF-κB axis to promote gastric cancer progression.
    Article Snippet: Infection with Helicobacter pylori (H. pylori) represents a significant risk factor for the development of gastric cancer (GC).. The oncogenic functions of MYBL2 have been reported in several malignancies, for which little research has focused on its effect on H. pylori-induced GC progression.. We analyzed the expression and clinical relevance of MYBL2 in GC.


    Article Title: Centipeda minima extracts and the active sesquiterpene lactones have therapeutic efficacy in non-small cell lung cancer by suppressing Skp2/p27 signaling pathway.
    Article Snippet: Ethnophamacological relevance: Centipeda minima (L.) A. Braun & Asch (C. minima) was applied to treat nasal allergy, headache, cough, and even nasopharyngeal carcinoma in traditional Chinese medicine.. However, the underlying anticancer mechanisms of C. minima and its active components have not been systematically

    Article Title: A Purely Biomanufactured System for Delivering Nanoparticles and STING Agonists.
    Article Snippet: .. After incubation with primary antibodies, the sections were treated with anti-mouse horseradish peroxidase (HRP)-conjugated antibodies and stained using a DAB Stain Kit (Solarbio) according to the manufacturer’s guidelines. .. Enzyme-linked immunosorbent assay (ELISA) Polystyrene 96-well flatbottom microtiter plates (Corning) were coated with 50 ng per well of pure Cap.

    Article Title: Adsorption Behavior of Carbon Dots on La3+ and The Multiple Effects on The Growth of Mung Bean Seedlings under La3+ Stress
    Article Snippet: The excessive use of rare earth elements (REEs) as micro-fertilizers is harmful to agricultural production and the environment.. This study explored the potential application of carbon dots (CDs) to mitigate the effects of La contamination.. The results indicate that the CDs based on citric acid (C-CDs) can adsorb La in an aqueous solution system through surface carboxyl and pyrrolic-N.

    Immunostaining:

    Article Title: Centipeda minima extracts and the active sesquiterpene lactones have therapeutic efficacy in non-small cell lung cancer by suppressing Skp2/p27 signaling pathway.
    Article Snippet: Ethnophamacological relevance: Centipeda minima (L.) A. Braun & Asch (C. minima) was applied to treat nasal allergy, headache, cough, and even nasopharyngeal carcinoma in traditional Chinese medicine.. However, the underlying anticancer mechanisms of C. minima and its active components have not been systematically

    Incubation:

    Article Title: A Purely Biomanufactured System for Delivering Nanoparticles and STING Agonists.
    Article Snippet: .. After incubation with primary antibodies, the sections were treated with anti-mouse horseradish peroxidase (HRP)-conjugated antibodies and stained using a DAB Stain Kit (Solarbio) according to the manufacturer’s guidelines. .. Enzyme-linked immunosorbent assay (ELISA) Polystyrene 96-well flatbottom microtiter plates (Corning) were coated with 50 ng per well of pure Cap.

    Multiple Displacement Amplification:

    Article Title: Adsorption Behavior of Carbon Dots on La3+ and The Multiple Effects on The Growth of Mung Bean Seedlings under La3+ Stress
    Article Snippet: The excessive use of rare earth elements (REEs) as micro-fertilizers is harmful to agricultural production and the environment.. This study explored the potential application of carbon dots (CDs) to mitigate the effects of La contamination.. The results indicate that the CDs based on citric acid (C-CDs) can adsorb La in an aqueous solution system through surface carboxyl and pyrrolic-N.

    Fluorescence:

    Article Title: Adsorption Behavior of Carbon Dots on La3+ and The Multiple Effects on The Growth of Mung Bean Seedlings under La3+ Stress
    Article Snippet: The excessive use of rare earth elements (REEs) as micro-fertilizers is harmful to agricultural production and the environment.. This study explored the potential application of carbon dots (CDs) to mitigate the effects of La contamination.. The results indicate that the CDs based on citric acid (C-CDs) can adsorb La in an aqueous solution system through surface carboxyl and pyrrolic-N.



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    Stromal CXCL12 associates with TLS enrichment in NSCLC-BM and improved outcomes with ICI therapy. (A) Bubble plots showing chemokine receptor expression levels in Microglia, B cells, and CD4-TCF1 T cells. (B) Bubble plots CXCL12 , CXCL13 , CCL19 , and CCL21 expression levels in NSCLC-BM samples. (C) Violin plots displaying the expression levels of CXCL12 and CXCL13 in tumours from TLS-low and TLS-high groups. TLS-low group, n = 12; TLS-high group, n = 13. P < 0.001 (∗∗∗). Dashed lines indicate the median and IQR. P -value was calculated using Mann–Whitney U test. P < 0.001 (∗∗∗). (D) Representative <t>IHC</t> images of CXCL12 staining in NSCLC-BM tissues. Scale bar = 100 μm. (E) The statistical results of CXCL12 IHC staining. Upper, Correlation between stromal CXCL12 expression and TLS scores.The stromal CXCL12 IRS scores of 0–6 were classified as low expression, whereas scores of 8–12 were classified as high expression. P -value was calculated using Pearson's chi-square test. Lower, the IRS of CXCL12 expression in paired stromal regions within and outside TLSs across 52 TLS-positive samples. Data are shown as individual values with mean ± SD. P -value was calculated using Wilcoxon test. (F) Bubble plot showing CXCL12 expression levels of different cell clusters. (G) Representative mIHC images of BM tissues stained with anti-CD20 (yellow), CD31 (red), FAP (magenta), and CXCL12 (green) antibodies. Upper, scale bar = 100 μm. Lower, scale bar = 10 μm. (H) Boxplots showing the fraction of CXCL12+ cells among CD31+ cells and FAP+ cells within TLSs across 52 TLS-positive samples. Data are presented as median with IQR. P -value was calculated using the Wilcoxon test. (I) Kaplan–Meier survival analysis for iPFS in patients with NSCLC-BM receiving postoperative ICIs (n = 29) according to CXCL12 IRS in intratumoral stroma. P -value was calculated using log-rank test. NSCLC-BM, non-small cell lung cancer brain metastasis; iPFS, intracranial progression-free survival; TLS, tertiary lymphoid structure; ICIs, immune checkpoint inhibitors; IRS, immunoreactivity scores.
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    Stromal CXCL12 associates with TLS enrichment in NSCLC-BM and improved outcomes with ICI therapy. (A) Bubble plots showing chemokine receptor expression levels in Microglia, B cells, and CD4-TCF1 T cells. (B) Bubble plots CXCL12 , CXCL13 , CCL19 , and CCL21 expression levels in NSCLC-BM samples. (C) Violin plots displaying the expression levels of CXCL12 and CXCL13 in tumours from TLS-low and TLS-high groups. TLS-low group, n = 12; TLS-high group, n = 13. P < 0.001 (∗∗∗). Dashed lines indicate the median and IQR. P -value was calculated using Mann–Whitney U test. P < 0.001 (∗∗∗). (D) Representative <t>IHC</t> images of CXCL12 staining in NSCLC-BM tissues. Scale bar = 100 μm. (E) The statistical results of CXCL12 IHC staining. Upper, Correlation between stromal CXCL12 expression and TLS scores.The stromal CXCL12 IRS scores of 0–6 were classified as low expression, whereas scores of 8–12 were classified as high expression. P -value was calculated using Pearson's chi-square test. Lower, the IRS of CXCL12 expression in paired stromal regions within and outside TLSs across 52 TLS-positive samples. Data are shown as individual values with mean ± SD. P -value was calculated using Wilcoxon test. (F) Bubble plot showing CXCL12 expression levels of different cell clusters. (G) Representative mIHC images of BM tissues stained with anti-CD20 (yellow), CD31 (red), FAP (magenta), and CXCL12 (green) antibodies. Upper, scale bar = 100 μm. Lower, scale bar = 10 μm. (H) Boxplots showing the fraction of CXCL12+ cells among CD31+ cells and FAP+ cells within TLSs across 52 TLS-positive samples. Data are presented as median with IQR. P -value was calculated using the Wilcoxon test. (I) Kaplan–Meier survival analysis for iPFS in patients with NSCLC-BM receiving postoperative ICIs (n = 29) according to CXCL12 IRS in intratumoral stroma. P -value was calculated using log-rank test. NSCLC-BM, non-small cell lung cancer brain metastasis; iPFS, intracranial progression-free survival; TLS, tertiary lymphoid structure; ICIs, immune checkpoint inhibitors; IRS, immunoreactivity scores.
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    Image Search Results


    Stromal CXCL12 associates with TLS enrichment in NSCLC-BM and improved outcomes with ICI therapy. (A) Bubble plots showing chemokine receptor expression levels in Microglia, B cells, and CD4-TCF1 T cells. (B) Bubble plots CXCL12 , CXCL13 , CCL19 , and CCL21 expression levels in NSCLC-BM samples. (C) Violin plots displaying the expression levels of CXCL12 and CXCL13 in tumours from TLS-low and TLS-high groups. TLS-low group, n = 12; TLS-high group, n = 13. P < 0.001 (∗∗∗). Dashed lines indicate the median and IQR. P -value was calculated using Mann–Whitney U test. P < 0.001 (∗∗∗). (D) Representative IHC images of CXCL12 staining in NSCLC-BM tissues. Scale bar = 100 μm. (E) The statistical results of CXCL12 IHC staining. Upper, Correlation between stromal CXCL12 expression and TLS scores.The stromal CXCL12 IRS scores of 0–6 were classified as low expression, whereas scores of 8–12 were classified as high expression. P -value was calculated using Pearson's chi-square test. Lower, the IRS of CXCL12 expression in paired stromal regions within and outside TLSs across 52 TLS-positive samples. Data are shown as individual values with mean ± SD. P -value was calculated using Wilcoxon test. (F) Bubble plot showing CXCL12 expression levels of different cell clusters. (G) Representative mIHC images of BM tissues stained with anti-CD20 (yellow), CD31 (red), FAP (magenta), and CXCL12 (green) antibodies. Upper, scale bar = 100 μm. Lower, scale bar = 10 μm. (H) Boxplots showing the fraction of CXCL12+ cells among CD31+ cells and FAP+ cells within TLSs across 52 TLS-positive samples. Data are presented as median with IQR. P -value was calculated using the Wilcoxon test. (I) Kaplan–Meier survival analysis for iPFS in patients with NSCLC-BM receiving postoperative ICIs (n = 29) according to CXCL12 IRS in intratumoral stroma. P -value was calculated using log-rank test. NSCLC-BM, non-small cell lung cancer brain metastasis; iPFS, intracranial progression-free survival; TLS, tertiary lymphoid structure; ICIs, immune checkpoint inhibitors; IRS, immunoreactivity scores.

    Journal: eBioMedicine

    Article Title: TCF1+CD4+ T cells and microglia co-orchestrate tertiary lymphoid structures to enhance prognosis and immunotherapy in non-small cell lung cancer with brain metastases

    doi: 10.1016/j.ebiom.2026.106280

    Figure Lengend Snippet: Stromal CXCL12 associates with TLS enrichment in NSCLC-BM and improved outcomes with ICI therapy. (A) Bubble plots showing chemokine receptor expression levels in Microglia, B cells, and CD4-TCF1 T cells. (B) Bubble plots CXCL12 , CXCL13 , CCL19 , and CCL21 expression levels in NSCLC-BM samples. (C) Violin plots displaying the expression levels of CXCL12 and CXCL13 in tumours from TLS-low and TLS-high groups. TLS-low group, n = 12; TLS-high group, n = 13. P < 0.001 (∗∗∗). Dashed lines indicate the median and IQR. P -value was calculated using Mann–Whitney U test. P < 0.001 (∗∗∗). (D) Representative IHC images of CXCL12 staining in NSCLC-BM tissues. Scale bar = 100 μm. (E) The statistical results of CXCL12 IHC staining. Upper, Correlation between stromal CXCL12 expression and TLS scores.The stromal CXCL12 IRS scores of 0–6 were classified as low expression, whereas scores of 8–12 were classified as high expression. P -value was calculated using Pearson's chi-square test. Lower, the IRS of CXCL12 expression in paired stromal regions within and outside TLSs across 52 TLS-positive samples. Data are shown as individual values with mean ± SD. P -value was calculated using Wilcoxon test. (F) Bubble plot showing CXCL12 expression levels of different cell clusters. (G) Representative mIHC images of BM tissues stained with anti-CD20 (yellow), CD31 (red), FAP (magenta), and CXCL12 (green) antibodies. Upper, scale bar = 100 μm. Lower, scale bar = 10 μm. (H) Boxplots showing the fraction of CXCL12+ cells among CD31+ cells and FAP+ cells within TLSs across 52 TLS-positive samples. Data are presented as median with IQR. P -value was calculated using the Wilcoxon test. (I) Kaplan–Meier survival analysis for iPFS in patients with NSCLC-BM receiving postoperative ICIs (n = 29) according to CXCL12 IRS in intratumoral stroma. P -value was calculated using log-rank test. NSCLC-BM, non-small cell lung cancer brain metastasis; iPFS, intracranial progression-free survival; TLS, tertiary lymphoid structure; ICIs, immune checkpoint inhibitors; IRS, immunoreactivity scores.

    Article Snippet: IHC staining was performed using the SP method, employing the universal immunohistochemical staining kit (Zhongshan Golden Bridge Biotechnology; Cat# PV-6000) and DAB chromogen kit (Zhongshan Golden Bridge Biotechnology; Cat# PV-6000D).

    Techniques: Expressing, MANN-WHITNEY, Staining, Immunohistochemistry