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polink 2 plus hrp rabbit  (OriGene)


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    Structured Review

    OriGene polink 2 plus hrp rabbit
    Polink 2 Plus Hrp Rabbit, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dab+kit/Polink-2+Plus+HRP+Rabbit+with+DAB+with+Kit/us12350322-720-55-62
    Average 94 stars, based on 37 article reviews
    polink 2 plus hrp rabbit - by Bioz Stars, 2026-11
    94/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Soybean Trypsin Inhibitor Possesses Potency Against SARS-CoV-2 Infection by Blocking the Host Cell Surface Receptors ACE2, TMPRSS2, and CD147.
    Article Snippet: Sections of lungs, livers, and kidneys removed after sacrifice were incubated with ACE2 primary antibody (bs-1004R diluted 50×; Bioss, Woburn, MA, USA), TMPRSS2 primary antibody (ab214462 diluted 200×; Abcam, Cambridge, UK), or CD147 primary antibody (R381696 diluted 50×; Zen-BioScience, Chengdu, China), respectively. .. IHC evaluation was performed using the Polink-2 Plus HRP Rabbit DAB Detection Kit (D39; OriGene, Rockville, MD, USA) according to the recommended protocol from the manufacturer, and microscopic examination of the results was conducted using a microscope (ECLIPSE TS100; Nikon, Japan), with images captured with a microscope camera (ProgRes camera; Jenoptik AG, Germany). ..

    Article Title: The efficacy of ophiopogonanone B in treating the cough in mice infected with Mycoplasma pneumoniae
    Article Snippet: The reference standard for Ophiopogonanone B was supplied by Wuhan Tianzhi Biotechnology Company (Lot No. 1316759-83-7, purity: ≥98%). .. A Rabbit Ultrasensitive Immunohistochemistry Kit (Beijing Zhongshan Jinqiao Biotechnology Company; Lot No. PV-9001), DAB Color Development Kit (Beijing Solarbio Science and Technology Company, lot no. DA1010), and TRIzol Reagent (GLPBIO, United States; Lot No. GK20008), UltraSYBR One-Step Fluorescent PCR Kit (Beijing Kangwei Century Biotechnology Company, Lot No. 29520), TRPA1 Polyclonal antibody (Proteintech, United States; Lot No. 19124-1-AP), Anti -CGRP antibody (Abcam, United Kingdom; Lot No. ab47027), Anti-Substance P antibody (Abcam, United Kingdom; Lot No. ab216414) were used. .. Based on a previous study , the BiacoreTM (GE Healthcare, United States) Test was used to chemically couple TRPA1 protein to a CM5 chip.

    Microscopy:

    Article Title: Soybean Trypsin Inhibitor Possesses Potency Against SARS-CoV-2 Infection by Blocking the Host Cell Surface Receptors ACE2, TMPRSS2, and CD147.
    Article Snippet: Sections of lungs, livers, and kidneys removed after sacrifice were incubated with ACE2 primary antibody (bs-1004R diluted 50×; Bioss, Woburn, MA, USA), TMPRSS2 primary antibody (ab214462 diluted 200×; Abcam, Cambridge, UK), or CD147 primary antibody (R381696 diluted 50×; Zen-BioScience, Chengdu, China), respectively. .. IHC evaluation was performed using the Polink-2 Plus HRP Rabbit DAB Detection Kit (D39; OriGene, Rockville, MD, USA) according to the recommended protocol from the manufacturer, and microscopic examination of the results was conducted using a microscope (ECLIPSE TS100; Nikon, Japan), with images captured with a microscope camera (ProgRes camera; Jenoptik AG, Germany). ..

    Polymer:

    Article Title: RAD23B Promotes Colorectal Cancer Metastasis via the Talin1/Integrin/PI3K/AKT/MMP9 Axis.
    Article Snippet: Background: Radiation sensitive 23 homolog B (RAD23B), a DNA repair-related protein, plays a contributory role in the development of multiple malignancies.. This study aimed to explore the role of RAD23B in promoting colorectal cancer (CRC) metastasis and to elucidate the underlying molecular mechanisms.. Methods: RAD23B was overexpressed in CRC cell lines SW480 and HCT-8, with empty vectors serving as controls.

    Polymerase Chain Reaction:

    Article Title: The efficacy of ophiopogonanone B in treating the cough in mice infected with Mycoplasma pneumoniae
    Article Snippet: The reference standard for Ophiopogonanone B was supplied by Wuhan Tianzhi Biotechnology Company (Lot No. 1316759-83-7, purity: ≥98%). .. A Rabbit Ultrasensitive Immunohistochemistry Kit (Beijing Zhongshan Jinqiao Biotechnology Company; Lot No. PV-9001), DAB Color Development Kit (Beijing Solarbio Science and Technology Company, lot no. DA1010), and TRIzol Reagent (GLPBIO, United States; Lot No. GK20008), UltraSYBR One-Step Fluorescent PCR Kit (Beijing Kangwei Century Biotechnology Company, Lot No. 29520), TRPA1 Polyclonal antibody (Proteintech, United States; Lot No. 19124-1-AP), Anti -CGRP antibody (Abcam, United Kingdom; Lot No. ab47027), Anti-Substance P antibody (Abcam, United Kingdom; Lot No. ab216414) were used. .. Based on a previous study , the BiacoreTM (GE Healthcare, United States) Test was used to chemically couple TRPA1 protein to a CM5 chip.

    Blocking Assay:

    Article Title: Peptide immunogens targeting interleukin 6 (IL-6) and formulations thereof for immunotherapy of diseases impacted by IL-6 dysregulation
    Article Snippet: Slide-mounted sections immersed in Antigen Retrieval Citrate Solution (Scytek) were heated until boiling in a microwave oven at maximum power and cooled down to room temperature for 30 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide/PBS for 10 min. .. Sections were preincubated with Ultravision Protein Block (ThermoFisher) at room temperature for 1 h. Then the sections were incubated with primary rabbit anti-rat IL-17 (Abbiotec, 1:100 diluted in PBST), anti-rat TNF-α (Abcam, 1:100 diluted in PBST) or anti-rat MCP-1 (Abcam, 1:200 diluted in PBST) at 4° C. overnight, washed with TBST (Scytek), and developed by Polink-2 Plus HRP Rabbit with DAB Kit (GBI Labs). .. The sections were counterstained with hematoxylin (Leica Biosystems), dehydrated and mounted in Surgipath Micromount mounting medium (Leica Biosystems).

    Incubation:

    Article Title: Peptide immunogens targeting interleukin 6 (IL-6) and formulations thereof for immunotherapy of diseases impacted by IL-6 dysregulation
    Article Snippet: Slide-mounted sections immersed in Antigen Retrieval Citrate Solution (Scytek) were heated until boiling in a microwave oven at maximum power and cooled down to room temperature for 30 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide/PBS for 10 min. .. Sections were preincubated with Ultravision Protein Block (ThermoFisher) at room temperature for 1 h. Then the sections were incubated with primary rabbit anti-rat IL-17 (Abbiotec, 1:100 diluted in PBST), anti-rat TNF-α (Abcam, 1:100 diluted in PBST) or anti-rat MCP-1 (Abcam, 1:200 diluted in PBST) at 4° C. overnight, washed with TBST (Scytek), and developed by Polink-2 Plus HRP Rabbit with DAB Kit (GBI Labs). .. The sections were counterstained with hematoxylin (Leica Biosystems), dehydrated and mounted in Surgipath Micromount mounting medium (Leica Biosystems).

    Expressing:

    Article Title: Chlorogenic acid attenuates endometrial senescence via mTOR modulation: A combined network pharmacology, in vitro, and in vivo study.
    Article Snippet: Advanced maternal age is correlated with increased infertility, often due to impaired decidualization and placentation.. This study elucidated the senotherapeutic potential of chlorogenic acid (CGA), a phenylacrylate polyphenol as major compound of coffee, in endometrial stromal cells (ESCs).. Using a passage number-induced senescence model, CGA reduced senescence markers (P16, P21), promoted proliferation, and upregulated decidualization markers (PRL, IGFBP1, FOXO1).

    other:

    Article Title: Quantitative imaging of schwannoma captures heterogeneity and accelerates preclinical testing, revealing distinct impacts of targeted therapies
    Article Snippet: The following secondary antibodies were used: Polink-2 plus HRP Syrian Hamster DAB (3,3’-Diaminobenzidine) Detection Kit (D86-18, OriGene) and Polink-2 plus HRP Rabbit DAB Detection Kit (D39-18, OriGene).



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