Review



situ apoptosis detection kit  (R&D Systems)


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    Structured Review

    R&D Systems situ apoptosis detection kit
    (A) TUNEL staining of kidney sections from Trpm2-KO and WT mice subjected to bilateral kidney ischemia or sham surgery. (B) Quantitation of apoptotic cells. ***P < 0.001. Scale bars: 50 μm. (C) Western blot analysis of kidney lysates from Trpm2-KO and WT mice subjected to bilateral kidney ischemia or sham surgery to detect pro- and antiapoptotic proteins. (D) Western blot analysis of PARP cleavage as a measure of <t>apoptosis.</t>
    Situ Apoptosis Detection Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dab+kit/TdT+In+Situ+Apoptosis+Detection+Kit+-+DAB/pmc04347231-396-13-17
    Average 94 stars, based on 137 article reviews
    situ apoptosis detection kit - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "TRPM2 mediates ischemic kidney injury and oxidant stress through RAC1"

    Article Title: TRPM2 mediates ischemic kidney injury and oxidant stress through RAC1

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI76042

    (A) TUNEL staining of kidney sections from Trpm2-KO and WT mice subjected to bilateral kidney ischemia or sham surgery. (B) Quantitation of apoptotic cells. ***P < 0.001. Scale bars: 50 μm. (C) Western blot analysis of kidney lysates from Trpm2-KO and WT mice subjected to bilateral kidney ischemia or sham surgery to detect pro- and antiapoptotic proteins. (D) Western blot analysis of PARP cleavage as a measure of apoptosis.
    Figure Legend Snippet: (A) TUNEL staining of kidney sections from Trpm2-KO and WT mice subjected to bilateral kidney ischemia or sham surgery. (B) Quantitation of apoptotic cells. ***P < 0.001. Scale bars: 50 μm. (C) Western blot analysis of kidney lysates from Trpm2-KO and WT mice subjected to bilateral kidney ischemia or sham surgery to detect pro- and antiapoptotic proteins. (D) Western blot analysis of PARP cleavage as a measure of apoptosis.

    Techniques Used: TUNEL Assay, Staining, Quantitation Assay, Western Blot

    Related Articles

    TUNEL Assay:

    Article Title: SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers
    Article Snippet: Slides were stained for peroxidase for 3 min with the DAB (Diamine-benzidine) substrate kit (Biocare Medical, DS900), washed with water and counterstained with hematoxylin. .. TUNEL staining (R&D Systems, 4810–30-K) was performed according to the manufacturer’s protocol. .. Slides were photographed with an Olympus DP74 microscope.

    Staining:

    Article Title: SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers
    Article Snippet: Slides were stained for peroxidase for 3 min with the DAB (Diamine-benzidine) substrate kit (Biocare Medical, DS900), washed with water and counterstained with hematoxylin. .. TUNEL staining (R&D Systems, 4810–30-K) was performed according to the manufacturer’s protocol. .. Slides were photographed with an Olympus DP74 microscope.



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