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double ihc staining kit  (OriGene)


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    Structured Review

    OriGene double ihc staining kit
    Double Ihc Staining Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dab+kit/Polink+DS-MR-Hu+A2+Kit+for+mouse+and+rabit+antibody+on+human+tissue+with+DAB+%26+GBI-Permanent+Red/pm40108601-45-24-29
    Average 93 stars, based on 6 article reviews
    double ihc staining kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: The correlation of tertiary lymphoid structures with tumor spread through air spaces and prognosis in lung adenocarcinoma: focusing on pathological spatial features.
    Article Snippet: .. The double CD20/CD23 IHC was performed using a primary antibody mixture of mouse anti-CD20 (Kit-0001, Maixin) and rabbit antiCD23 (18642-1-AP, Proteintech), along with a double IHC staining kit (DS-0004, Zhongshan Golden Bridge), and the procedures were performed according to the instructions. ..

    Article Title: Down‐regulation of Shh in the hair follicles of mice during chemotherapy‐induced hair loss is mediated by the JAK / STAT1 signaling pathway
    Article Snippet: .. For immunohistochemistry staining, paraffin sections were prepared, an anti‐p53 primary antibody (#2524; CST, Danvers, MA, USA) and a 3,3′‐diaminobenzidine staining kit (ZSBio, Beijing, China) were used. .. For immunofluorescence staining, cryosections were prepared and incubated with an anti‐activated caspase‐3 primary antibody (AF835; R&D Systems, Minneapolis, MN, USA) at 4 °C overnight.

    Staining:

    Article Title: The correlation of tertiary lymphoid structures with tumor spread through air spaces and prognosis in lung adenocarcinoma: focusing on pathological spatial features.
    Article Snippet: .. The double CD20/CD23 IHC was performed using a primary antibody mixture of mouse anti-CD20 (Kit-0001, Maixin) and rabbit antiCD23 (18642-1-AP, Proteintech), along with a double IHC staining kit (DS-0004, Zhongshan Golden Bridge), and the procedures were performed according to the instructions. ..

    Article Title: Analysis and verification of potential ferroptosis-related diagnostic markers in the early stage of metabolic dysfunction-associated steatohepatitis.
    Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH) is currently the fastest-growing cause of liver-related deaths.. Intervention in the early stage of MASH will effectively reverse the condition and avoid huge health and economic burdens.. To date, our understanding of MASH remains quite limited.

    Article Title: Down‐regulation of Shh in the hair follicles of mice during chemotherapy‐induced hair loss is mediated by the JAK / STAT1 signaling pathway
    Article Snippet: .. For immunohistochemistry staining, paraffin sections were prepared, an anti‐p53 primary antibody (#2524; CST, Danvers, MA, USA) and a 3,3′‐diaminobenzidine staining kit (ZSBio, Beijing, China) were used. .. For immunofluorescence staining, cryosections were prepared and incubated with an anti‐activated caspase‐3 primary antibody (AF835; R&D Systems, Minneapolis, MN, USA) at 4 °C overnight.

    Article Title: Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration.
    Article Snippet: .. Afterward, the staining process proceeded with the application of a universal two-step staining kit (Zhongshan Golden Bridge, PV-9000, Beijing, China), which included all reagents except the primary antibodies. .. The primary antibodies used in this study were as delineated below: antiCD11b (human/mouse, 1:4000, Abcam, ab133357, UK), anti-Collagen Iα1 (mouse, 1:500, Abcam, ab270993, UK), anti-Collagen II (mouse, 1:400, Abcam, ab34712, UK), anti-IL-1α (mouse, 1:500, Abcam, ab300499, UK), and anti-TNF-α (mouse, 2.5 μg/mL, Abcam, ab1793, UK).

    Incubation:

    Article Title: Interleukin 6 induces M2 macrophage differentiation by STAT3 activation that correlates with gastric cancer progression
    Article Snippet: .. Sections were pre-incubated with normal goat serum at 37 °C for 30 min followed by incubation with primary mouse anti-IL-6 antibody (Abcam, Cambridge, MA, USA) or anti-CD163 rabbit monoclonal antibody (Abcam) overnight at 4 °C, and then incubated with horseradish peroxidase (HRP-) conjugated anti-mouse or anti-rabbit secondary antibody (Zhongshan Biotechnology, Beijing, China) at 37 °C for 30 min. Polink DS-MR-Hu A2 Kit (Zhongshan Biotechnology) was used for double staining of CD163 and CD206 with anti-CD163 rabbit antibody and anti-CD206 mouse antibody (Abcam) according to the manufacturer’s instructions. ..

    Article Title: Organoid culture of mouse fallopian tube epithelial stem cells with a thermo-reversible gelation polymer.
    Article Snippet: In this study, a three-dimensional (3D) thermo-reversible gelation polymer (TGP) culture system was established for organoid culture of mouse fallopian tube (FT) epithelial stem cells (FTESCs) without cell isolation.. FT tissues from 6to 8-week-old ICR mice were digested with collagenase, and whole FT cells (FTCs) were inoculated into the TGP.. After 6 days of culture, many spheres in the TGP formed.

    Double Staining:

    Article Title: Interleukin 6 induces M2 macrophage differentiation by STAT3 activation that correlates with gastric cancer progression
    Article Snippet: .. Sections were pre-incubated with normal goat serum at 37 °C for 30 min followed by incubation with primary mouse anti-IL-6 antibody (Abcam, Cambridge, MA, USA) or anti-CD163 rabbit monoclonal antibody (Abcam) overnight at 4 °C, and then incubated with horseradish peroxidase (HRP-) conjugated anti-mouse or anti-rabbit secondary antibody (Zhongshan Biotechnology, Beijing, China) at 37 °C for 30 min. Polink DS-MR-Hu A2 Kit (Zhongshan Biotechnology) was used for double staining of CD163 and CD206 with anti-CD163 rabbit antibody and anti-CD206 mouse antibody (Abcam) according to the manufacturer’s instructions. ..

    Immunohistochemical staining:

    Article Title: Organoid culture of mouse fallopian tube epithelial stem cells with a thermo-reversible gelation polymer.
    Article Snippet: In this study, a three-dimensional (3D) thermo-reversible gelation polymer (TGP) culture system was established for organoid culture of mouse fallopian tube (FT) epithelial stem cells (FTESCs) without cell isolation.. FT tissues from 6to 8-week-old ICR mice were digested with collagenase, and whole FT cells (FTCs) were inoculated into the TGP.. After 6 days of culture, many spheres in the TGP formed.

    Article Title: Analysis and verification of potential ferroptosis-related diagnostic markers in the early stage of metabolic dysfunction-associated steatohepatitis.
    Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH) is currently the fastest-growing cause of liver-related deaths.. Intervention in the early stage of MASH will effectively reverse the condition and avoid huge health and economic burdens.. To date, our understanding of MASH remains quite limited.

    Expressing:

    Article Title: Analysis and verification of potential ferroptosis-related diagnostic markers in the early stage of metabolic dysfunction-associated steatohepatitis.
    Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH) is currently the fastest-growing cause of liver-related deaths.. Intervention in the early stage of MASH will effectively reverse the condition and avoid huge health and economic burdens.. To date, our understanding of MASH remains quite limited.



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