other:Article Title: Targeting CDK2 ameliorates allergic asthma through suppression of epithelial chemokine production and T cell inflammatory responses.
Article Snippet: 1Department of Biochemistry and Department of Pulmonology, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China 2Postgraduate Training Base Alliance of Wenzhou Medical University (Zhejiang Cancer Hospital), Hangzhou 310022, China 3Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou 310022, China 4Affiliated Hangzhou First People’s Hospital, School of Medicine, Westlake University, Hangzhou 310006, China 5Department of Immunology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China 6Department of Pulmonology, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China 7Key Laboratory of Scientific Technology and Intelligent Equipment for Prevention and Control of Major Chronic Diseases, Ministry of Industry and Information Technology, Harbin Institute of Technology, Zhengzhou 450000, China 8Guangxi Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor, Nanning 530021, China 9Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning 530021, China
CCK-8 Assay:
Flow Cytometry:
Analysis:
Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
Staining:
Article Title: Sinensetin ameliorates osteoarthritis progression by modulating the NF-κB/NLRP3 signaling pathway and suppressing chondrocyte pyroptosis.
Article Snippet: Recombinant human IL-1β (catalog no. 200-1B) was obtained from PeproTech (Monmouth Junction, NJ, USA), and the Cell Counting Kit-8 (CCK-8) was sourced from Dojindo Laboratories (Kumamoto, Japan). .. Human IL-1β and IL-18 ELISA kits were purchased from Absin (Shanghai, China), whereas TUNEL staining, LDH release, and cell cycle analysis kits were supplied by Beyotime Biotechnology (Shanghai, China). .. Primary antibodies against ASC, NLRP3, GSDMD, SERPINA3, GAPDH, and β-actin were acquired from Proteintech (Wuhan, China), and secondary antibodies conjugated with Alexa Fluor 488 or 594 were obtained from Beyotime.
Immunofluorescence:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
β-Gal Assay:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
Nile Red Staining:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
Protein Extraction:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
Tissue:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
Cell Culture:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
BIA-KA:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
RNA Extraction:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
Multiple Displacement Amplification:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
Software:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
Article Snippet: The CCR6 antagonist (Cat# HY-151435, MedChem Express; 1mg/kg) was intraperitoneally administered twice a week until mice were euthanized; AZ191 (Cat# HY-12277, MedChem .. REAGENT or RESOURCE SOURCE IDENTIFIER PBS Solarbio Cat #P1020 RPMI 1640 medium Solarbio Cat #31800 Critical commercial assays Cell Cycle and Apoptosis Analysis Kit Beyotime Cat #C1052 DNA Damage Assay Kit by γ-H2AX Immunofluorescence Beyotime Cat #C2037S Senescence β-Galactosidase Staining Kit Beyotime Cat #C0602 Nile Red Staining Kit Beyotime Cat #C2051S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat #P0028 REAGENT or RESOURCE SOURCE IDENTIFIER MinuteTM ER Enrichment Kit For Tissue and Cultured Cells Invent Biotech Cat #ER-036 Minute Golgi Apparatus Enrichment Kit Invent Biotech Cat #GO-037 BCA protein assay kit Thermo Scientific Cat #23227 Omni-ECLTM Femto Light Chemiluminescence Kit Epizyme Biotech Cat #SQ201 SteadyPure Rapid RNA Extraction Kit Accurate biology Cat #AG21023 Experimental models: Cell lines MDA-MB-231 ATCC Cat #HTB-26 MDA-MB-468 ATCC Cat #HTB-132 HCC1937 ATCC Cat #CRL-2336 BT-549 ATCC Cat #HTB-122 HEK-293T ATCC Cat #CRL-3216 4T1 ATCC Cat #CRL-2539 Py230 ATCC Cat #CRL-3279 Experimental models: Organisms/strains Mouse: Balb/c HFK Bioscience Cat #11002A Mouse: C57BL/6(MMTV-PyMT) Cyagen Cat #C001212 Mouse: Mmgtfl/fl; MMTV-Cre; PyMT Cyagen Cat #S-CKO-07890 Mouse: Balb/c nude SPF (Beijing) biotechnology Cat #C201 Mouse: C-NKG Cyagen Cat #C001316 Mouse: FVB SPF (Beijing) biotechnology Cat #B210 Software and algorithms R version 4.2.3 V4.3.1 https://www.r-project.org/ GraphPad Prism 9.3 GraphPad Software https://www.graphpad.com/ Image J v1.53 NIH https://imagej.nih.gov/ij/ FlowJo v10.8.1 BD Biosciences https://www.flowjo.com e3 Cell Reports Medicine 7, 103120, November 17, 2026 Article ll OPEN ACCESS Express; 50mg/kg) was intraperitoneally administered every day for ten days. .. Anti-PD-1 antibody (Cat# A2122, Selleck; 100 μg/ mouse) was intraperitoneally administered four times within two weeks.
Enzyme-linked Immunosorbent Assay:Article Title: Sinensetin ameliorates osteoarthritis progression by modulating the NF-κB/NLRP3 signaling pathway and suppressing chondrocyte pyroptosis.
Article Snippet: Recombinant human IL-1β (catalog no. 200-1B) was obtained from PeproTech (Monmouth Junction, NJ, USA), and the Cell Counting Kit-8 (CCK-8) was sourced from Dojindo Laboratories (Kumamoto, Japan). .. Human IL-1β and IL-18 ELISA kits were purchased from Absin (Shanghai, China), whereas TUNEL staining, LDH release, and cell cycle analysis kits were supplied by Beyotime Biotechnology (Shanghai, China). .. Primary antibodies against ASC, NLRP3, GSDMD, SERPINA3, GAPDH, and β-actin were acquired from Proteintech (Wuhan, China), and secondary antibodies conjugated with Alexa Fluor 488 or 594 were obtained from Beyotime.
TUNEL Assay:Article Title: Sinensetin ameliorates osteoarthritis progression by modulating the NF-κB/NLRP3 signaling pathway and suppressing chondrocyte pyroptosis.
Article Snippet: Recombinant human IL-1β (catalog no. 200-1B) was obtained from PeproTech (Monmouth Junction, NJ, USA), and the Cell Counting Kit-8 (CCK-8) was sourced from Dojindo Laboratories (Kumamoto, Japan). .. Human IL-1β and IL-18 ELISA kits were purchased from Absin (Shanghai, China), whereas TUNEL staining, LDH release, and cell cycle analysis kits were supplied by Beyotime Biotechnology (Shanghai, China). .. Primary antibodies against ASC, NLRP3, GSDMD, SERPINA3, GAPDH, and β-actin were acquired from Proteintech (Wuhan, China), and secondary antibodies conjugated with Alexa Fluor 488 or 594 were obtained from Beyotime.
Cell Cycle Assay:Article Title: Sinensetin ameliorates osteoarthritis progression by modulating the NF-κB/NLRP3 signaling pathway and suppressing chondrocyte pyroptosis.
Article Snippet: Recombinant human IL-1β (catalog no. 200-1B) was obtained from PeproTech (Monmouth Junction, NJ, USA), and the Cell Counting Kit-8 (CCK-8) was sourced from Dojindo Laboratories (Kumamoto, Japan). .. Human IL-1β and IL-18 ELISA kits were purchased from Absin (Shanghai, China), whereas TUNEL staining, LDH release, and cell cycle analysis kits were supplied by Beyotime Biotechnology (Shanghai, China). .. Primary antibodies against ASC, NLRP3, GSDMD, SERPINA3, GAPDH, and β-actin were acquired from Proteintech (Wuhan, China), and secondary antibodies conjugated with Alexa Fluor 488 or 594 were obtained from Beyotime.
Propidium Iodide Staining:
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