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cell cycle staining solution  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Structured Review

    Multi Sciences (Lianke) Biotech Co Ltd cell cycle staining solution
    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
    Cell Cycle Staining Solution, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 98/100, based on 1482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cycle+kit/Cell+cycle+staining+Kit/pmc12963903-77-56-59
    Average 98 stars, based on 1482 article reviews
    cell cycle staining solution - by Bioz Stars, 2026-11
    98/100 stars

    Images

    1) Product Images from "A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration"

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.051

    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
    Figure Legend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Techniques Used: In Vitro, Fluorescence, Staining

    Related Articles

    Binding Assay:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Flow Cytometry:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Staining:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Centrifugation:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Cytometry:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Transfection:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Analysis:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Incubation:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Cell Cycle Assay:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Control:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Oligomer Restriction:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    EdU Assay:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    BIA-KA:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Transferring:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Standard Curve:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Software:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio

    Lactate Dehydrogenase Assay:

    Article Title: Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses—In Vitro and Cell Line Xenograft Evidence
    Article Snippet: ls were analyzed by flow cytometry. The apoptotic population was calculated as the sum of early and late apoptotic cells. Cell-cycle distribution was evaluated using a cell-cycle staining kit (CCS012; LIANKE Biotech). Treated cells were detached using EDTA-free trypsin (C100C1; New Cell & Molecular Biotech, Suzhou, China), collected by centrifugation, and resuspended in 1 mL of DNA staining solutio



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