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label it plasmid delivery control cy3  (Mirus Bio)


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    Structured Review

    Mirus Bio label it plasmid delivery control cy3
    Label It Plasmid Delivery Control Cy3, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 96/100, based on 1903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy3+kit/Label+IT+Nucleic+Acid+Labeling+Kit/pm41344099-56-0-8
    Average 96 stars, based on 1903 article reviews
    label it plasmid delivery control cy3 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Labeling:

    Article Title: Unveiling a missing component of the atypical type IV secretion system required for natural transformation of Helicobacter pylori
    Article Snippet: .. When cultures reached exponential phase (18-24h), OD was normalized to 4 in 20 μL and 200 ng of λ-DNA (New England Biolabs), labelled with Cy3 using Label IT Nucleic Acid Labeling Kit (Mirus Bio) according to manufacturer instructions, were added to the reaction. ..

    Article Title: Cu(II) and Zn(II) Enhance Antibiotic Resistance Gene Transformation by Regulating Type IV Pili-Mediated DNA Uptake.
    Article Snippet: Natural transformation represents one of the major dissemination pathways of antibiotic resistance genes (ARGs) in the environment.. It can be facilitated by heavy metals, which is traditionally attributed to the induction of oxidative stress and an SOS response.. Here, we demonstrate an alternative mechanism involving type IV pili (T4P), the molecular machinery for extracellular DNA (eDNA) uptake.

    Article Title: Ionizable Polymeric Micelles Targeting Transferrin Receptor 1 Enhance Systemic mRNA Delivery to the Brain.
    Article Snippet: The samples were incubated at 37 °C for 1 h, followed by RNA extraction using the RNeasy Mini Kit. cDNA was then synthesized through reverse transcription using the High Capacity RNA-to-cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. Finally, qPCR was performed on an Applied Biosystems 7500 Fast Real-Time PCR System us i ng the spe c i fi ed p r ime r/p robe pa i r s : (Fo rwa rd : GTGGTGTGCAGCGAGAATAG, Reverse: CGCTCGTTGTAGATGTCGTTAG, Probe: TTGCAGTTCTTCATGCCCGTGTTG) To evaluate the structural stability of mRNA/PMs in serum, mRNA was double labeled with Cy3 and Cy5 using the Label IT Cy3 Labeling Kit and Label IT Cy5 Labeling Kit (Mirus Bio LLC, Madison, WI), followed by encapsulation into PMs. .. Before and after incubation in 50% FBS at 37 °C, samples were excited at 520 nm, and fluorescence emission spectra were recorded using Infinite M Nano (Tecan Group Ltd., Zürich, Switzerland).

    Article Title: Ionizable Polymeric Micelles Targeting Transferrin Receptor 1 Enhance Systemic mRNA Delivery to the Brain.
    Article Snippet: .. mRNA was labeled with Cy5 using the Label IT Cy5 Labeling Kit (Mirus Bio LLC, Madison, WI) and subsequently encapsulated in PMs as described above. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Ionizable Polymeric Micelles Targeting Transferrin Receptor 1 Enhance Systemic mRNA Delivery to the Brain.
    Article Snippet: The samples were incubated at 37 °C for 1 h, followed by RNA extraction using the RNeasy Mini Kit. cDNA was then synthesized through reverse transcription using the High Capacity RNA-to-cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. Finally, qPCR was performed on an Applied Biosystems 7500 Fast Real-Time PCR System us i ng the spe c i fi ed p r ime r/p robe pa i r s : (Fo rwa rd : GTGGTGTGCAGCGAGAATAG, Reverse: CGCTCGTTGTAGATGTCGTTAG, Probe: TTGCAGTTCTTCATGCCCGTGTTG) To evaluate the structural stability of mRNA/PMs in serum, mRNA was double labeled with Cy3 and Cy5 using the Label IT Cy3 Labeling Kit and Label IT Cy5 Labeling Kit (Mirus Bio LLC, Madison, WI), followed by encapsulation into PMs. .. Before and after incubation in 50% FBS at 37 °C, samples were excited at 520 nm, and fluorescence emission spectra were recorded using Infinite M Nano (Tecan Group Ltd., Zürich, Switzerland).

    Encapsulation:

    Article Title: Ionizable Polymeric Micelles Targeting Transferrin Receptor 1 Enhance Systemic mRNA Delivery to the Brain.
    Article Snippet: The samples were incubated at 37 °C for 1 h, followed by RNA extraction using the RNeasy Mini Kit. cDNA was then synthesized through reverse transcription using the High Capacity RNA-to-cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. Finally, qPCR was performed on an Applied Biosystems 7500 Fast Real-Time PCR System us i ng the spe c i fi ed p r ime r/p robe pa i r s : (Fo rwa rd : GTGGTGTGCAGCGAGAATAG, Reverse: CGCTCGTTGTAGATGTCGTTAG, Probe: TTGCAGTTCTTCATGCCCGTGTTG) To evaluate the structural stability of mRNA/PMs in serum, mRNA was double labeled with Cy3 and Cy5 using the Label IT Cy3 Labeling Kit and Label IT Cy5 Labeling Kit (Mirus Bio LLC, Madison, WI), followed by encapsulation into PMs. .. Before and after incubation in 50% FBS at 37 °C, samples were excited at 520 nm, and fluorescence emission spectra were recorded using Infinite M Nano (Tecan Group Ltd., Zürich, Switzerland).

    CCK-8 Assay:

    Article Title: Transfersomes with core and surface‐loaded NF‐κB p65 siRNA for enhanced transdermal transfection and effective treatment of psoriasis
    Article Snippet: DMEM, trypsin, fetal bovine serum, and penicillin‐streptomycin were purchased from Gibco. .. Mirus Label IT® reagent kits were purchased from XinboSheng Bio‐Tech Co., Ltd. CCK‐8 assay kits, Lyso‐Tracker kits, and NF‐κB p65 assay kits were purchased from Biyuntian Bio‐Tech Co., Ltd. Dio reagent kits were purchased from KeyGEN BioTECH Co., Ltd. .. Mouse anti‐Ki67, VEGF, IL‐6, IL‐23, IL‐17A, and TNF‐α antibodies were purchased from SanYing Bio‐Tech Co., Ltd. HaCat, Raw264.7, and 293T cells were purchased from Meisen Cell Technology Co., Ltd. Chlorpromazine, Genistein, Wortmannin, and Cytochalasin B were purchased from MedChem Express.

    other:

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: The Cy3-DNA was obtained from Mirus Biosciences (MIR 7905), while MFP488-DNA was labeled according to the manufacturers protocol and purified via ethanol precipitation (Mirus MIR 7125).



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    Image Search Results


    (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with UTP-X-Cy3 labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.

    Journal: bioRxiv

    Article Title: Restoring Shugoshin 1 reduces chromosome errors in human eggs

    doi: 10.64898/2026.01.08.698387

    Figure Lengend Snippet: (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with UTP-X-Cy3 labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.

    Article Snippet: The HighYield T7 Cy3 RNA Labelling Kit (Jena Bioscience, #RNT-101-CY3) was used to label amplified sequences with Cy3-labelled UTP (UTP-X-Cy3, 35%), followed by DNA removal using TurboTM DNAse (ThermoFisher).

    Techniques: Immunofluorescence, Negative Control, Control, Microinjection, Labeling

    Effect of inflammation on spermatogonia in LPS-induced mouse testes. ( A ) Testes from LPS-induced mice (Orchitis) and the Sham group. n = 6 mice per group ( B ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. n = 6 mice per group ( C ) Expression of SP1 at the protein level as detected by Western blot. n = 6 mice per group ( D ) H&E staining was used to analyze the histopathology of the testes. n = 3 mice per group ( E ) Apoptosis in testes was analyzed using TUNEL staining. n = 3 mice per group (F-I) Expression levels of Oct4, C-kit, IL-17 A and ZO-1 as detected by IHC assay. n = 3 mice per group. Statistical comparisons between the two groups were performed using an unpaired two-tailed Student’s t-test. *** P < 0.001

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

    doi: 10.1007/s00018-025-06055-3

    Figure Lengend Snippet: Effect of inflammation on spermatogonia in LPS-induced mouse testes. ( A ) Testes from LPS-induced mice (Orchitis) and the Sham group. n = 6 mice per group ( B ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. n = 6 mice per group ( C ) Expression of SP1 at the protein level as detected by Western blot. n = 6 mice per group ( D ) H&E staining was used to analyze the histopathology of the testes. n = 3 mice per group ( E ) Apoptosis in testes was analyzed using TUNEL staining. n = 3 mice per group (F-I) Expression levels of Oct4, C-kit, IL-17 A and ZO-1 as detected by IHC assay. n = 3 mice per group. Statistical comparisons between the two groups were performed using an unpaired two-tailed Student’s t-test. *** P < 0.001

    Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

    Techniques: Expressing, Western Blot, Staining, Histopathology, TUNEL Assay, Two Tailed Test

    Effects of LPS-induced inflammation on spermatogonia in vitro. (A) Cell proliferation was detected by EdU staining. (B-C) Apoptosis was detected using flow cytometry and the TUNEL assay. (D) SP1 expression at the protein level was detected using a Western blot. (E) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. (F–H) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical comparisons between the two groups were performed using an unpaired two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

    doi: 10.1007/s00018-025-06055-3

    Figure Lengend Snippet: Effects of LPS-induced inflammation on spermatogonia in vitro. (A) Cell proliferation was detected by EdU staining. (B-C) Apoptosis was detected using flow cytometry and the TUNEL assay. (D) SP1 expression at the protein level was detected using a Western blot. (E) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. (F–H) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical comparisons between the two groups were performed using an unpaired two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001

    Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

    Techniques: In Vitro, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot, Two Tailed Test

    SP1 knockdown reduces the LPS-induced spermatogonia dysfunction. ( A ) Cell proliferation was detected using EdU staining. ( B - C ) Cell apoptosis was detected using flow cytometry and the TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. ( E ) SP1 expression at the protein level was detected by Western blot. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

    doi: 10.1007/s00018-025-06055-3

    Figure Lengend Snippet: SP1 knockdown reduces the LPS-induced spermatogonia dysfunction. ( A ) Cell proliferation was detected using EdU staining. ( B - C ) Cell apoptosis was detected using flow cytometry and the TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. ( E ) SP1 expression at the protein level was detected by Western blot. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

    Techniques: Knockdown, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

    SNHG1 knockdown reduces LPS-induced spermatogonial dysfunction. ( A ) Cell proliferation was detected by EdU staining. ( B - C ) Cell apoptosis was detected by flow cytometry and TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected by qPCR. ( E ) SP1 expression at the protein level was detected by Western blot. ( F – H ) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

    doi: 10.1007/s00018-025-06055-3

    Figure Lengend Snippet: SNHG1 knockdown reduces LPS-induced spermatogonial dysfunction. ( A ) Cell proliferation was detected by EdU staining. ( B - C ) Cell apoptosis was detected by flow cytometry and TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected by qPCR. ( E ) SP1 expression at the protein level was detected by Western blot. ( F – H ) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

    Techniques: Knockdown, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

    IL-17A signaling pathway blockade rescued the SNHG1 -induced cell dysfunction. ( A ) Cell proliferation was detected using EdU staining. ( B - C ) Cell apoptosis detected by flow cytometry and TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected by qPCR. ( E ) SP1 expression at the protein level was detected using a Western blot. ( F – H ) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, ** P < 0.01, *** P < 0.001

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

    doi: 10.1007/s00018-025-06055-3

    Figure Lengend Snippet: IL-17A signaling pathway blockade rescued the SNHG1 -induced cell dysfunction. ( A ) Cell proliferation was detected using EdU staining. ( B - C ) Cell apoptosis detected by flow cytometry and TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected by qPCR. ( E ) SP1 expression at the protein level was detected using a Western blot. ( F – H ) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, ** P < 0.01, *** P < 0.001

    Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

    Techniques: Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

    Re-expressing SNHGI in SP1-knock-down cells protect cells from inflammatory damage. ( A - C ) Expression levels of SP1 , SNHG1 , and IL-17 A were detected by QPCR assay. ( D ) The WB method was employed to assess the protein expression levels of SP1 and IL-17 A . ( E ) EdU staining was utilized to evaluate cell proliferation. ( F ) Flow cytometry was applied to detect cell apoptosis. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, ** P < 0.01, *** P < 0.001

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

    doi: 10.1007/s00018-025-06055-3

    Figure Lengend Snippet: Re-expressing SNHGI in SP1-knock-down cells protect cells from inflammatory damage. ( A - C ) Expression levels of SP1 , SNHG1 , and IL-17 A were detected by QPCR assay. ( D ) The WB method was employed to assess the protein expression levels of SP1 and IL-17 A . ( E ) EdU staining was utilized to evaluate cell proliferation. ( F ) Flow cytometry was applied to detect cell apoptosis. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, ** P < 0.01, *** P < 0.001

    Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

    Techniques: Expressing, Knockdown, Staining, Flow Cytometry