x cutsmart buffer (New England Biolabs)

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New England Biolabs x cutsmart buffer
X Cutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1007 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cutsmart+buffer/rCutSmart+Buffer/pm42881182-488-16-19
Average 99 stars, based on 1007 article reviews

x cutsmart buffer - by Bioz Stars, 2026-11

99/100 stars

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Incubation:

Article Title: G-quadruplex structures affect the immune response to bacterial infection in mouse macrophages
Article Snippet: Streptavidin Dynabeads M280 (Invitrogen, #10202983) were washed with incubation buffer, added to the sample, and rotated for 4 h at 4 °C. .. To reduce genomic background, beads were washed twice with 1× rCutSmart buffer (NEB, #B6004S), resuspended in 100 μl of the same buffer with 2 U double-strand-specific dsDNase (Sigma Aldrich, #EN771), and incubated for 10 min at 37 °C with shaking, followed by three washes with cold incubation buffer. ..

Plasmid Preparation:

Article Title: A Rapid Method to Rank Proteins of Widely Varying Stability: Application to Paramyxovirus Foot Domains.
Article Snippet: .. suBLA vector miniprep (see Strategic Planning) 10× restriction enzyme buffer (e.g, rCutSmart Buffer, New England Biolabs, cat. no. B6004S, supplied with BSER1) H2O, RNase-free BseR1 restriction enzyme (e.g, New England Biolabs, cat. no. R0581S)Hausrath et al. 8 of 28 Current Protocols nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70479 by M ukul C hauhan - U niversity O f D elhi , on [24/10/2026] .. Alkaline phosphatase, calf or shrimp (e.g, New England Biolabs, cat. nos

Article Title: Reconstituting human and SV40 replisomes with Okazaki fragment maturation reveals synchronized leading- and lagging-strand synthesis.
Article Snippet: .. ZN38 plasmid was digested with BsaI (20,000 units/ml, New England Biolabs) in a reaction containing 1 x CutSmart Buffer (New England Biolabs) for 2 h at 37 °C. .. The digested plasmid was purified from an agarose gel using the QIAquick Gel Extraction Kit (QIAGEN).

Recombinant:

Article Title: Rheology and programmable gelation of DNA tadpoles.
Article Snippet: .. The cutting mixture contained: 50 nM scaffold, 0.4 U mL 1 EcoRI-HF, and 1 rCutSmart buffer (50 mM Potassium Acetate, 20 mM Trisacetate, 10 mM Magnesium Acetate, 100 mg mL 1 Recombinant Albumin) (NEB B6004). ..

Purification:

Article Title: Single Staphylococcus aureus SauUSI restriction enzyme motors fragment methylated DNA.
Article Snippet: .. Following purification (Zymo clean and concentrate kit), the non-SR DNA circle was linearized and fragmented with EcoRI-HF (NEB) and 10 nM RecBCD (gift from M. Dillingham) in rCutSmart buffer (NEB) supplemented with 1 mM ATP for 1.5 h at 37 °C. .. SRs were purified using AMPure XP beads (Beckman Coulter).

Construct:

Article Title: Myopathy-causing variants of PYROXD1 converge on impaired RNA ligation
Article Snippet: Primers for site-directed mutagenesis of constructs were designed with NEBaseChanger ( https://nebasechanger.neb.com/ ) and the linear mutagenized constructs were generated with Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493) from the non-mutagenized constructs. .. For circularization of linear mutagenized constructs, 2 μL of the unpurified PCR product was mixed with 2 μL 10x rCutSmart (NEB, B6004), 2 μL 10 mM ATP, 1 μL 100 mM DTT, 1 μL T4 DNA Ligase (NEB, M0202), 0.2 μL T4 PNK (NEB, M0201), 0.2 μL DpnI (NEB, R0176) and 11.6 μL nuclease-free water. ..

Polymerase Chain Reaction:

other:

Article Title: Multiplexed single-cell reduced representation bisulfite sequencing for genome-wide DNA methylation profiling.
Article Snippet: DNA methylation is a fundamental epigenetic modification that regulates gene expression, maintains genomic integrity and defines cellular identity.. Conventional bulk sequencing methods, such as reduced-representation bisulfite sequencing (RRBS), have elucidated genome-wide methylation landscapes but do not reveal cell-to-cell variation, limiting insights into methylation heterogeneity during development, differentiation and disease.. Here, we present multiplexed single-cell RRBS (Msc-RRBS), a streamlined and scalable protocol for parallel DNA methylation profiling of hundreds of individual cells.

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