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e3lite customizable ubiquitin ligase kit  (LifeSensors)

 
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    Structured Review

    LifeSensors e3lite customizable ubiquitin ligase kit
    E3lite Customizable Ubiquitin Ligase Kit, supplied by LifeSensors, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/customised+oligonucleotide+microarray/e3lite+customizable+ubiquitin+ligase+kit/pm29681526-239-19-25
    Average 90 stars, based on 1 article reviews
    e3lite customizable ubiquitin ligase kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Ubiquitin Proteomics:

    Article Title: Recognition and reprogramming of E3 ubiquitin ligase surfaces by α-helical peptides
    Article Snippet: .. E3LITE Customizable Ubiquitin Ligase Kit (LifeSensors, UC101) was used to assess the autoubiquitination activity of HECT domain of WWP2 (WWP2 HECT ). ..

    Article Title: p140Cap modulates the mevalonate pathway decreasing cell migration and enhancing drug sensitivity in breast cancer cells
    Article Snippet: .. The ubiquitination assay was performed on 100 μg of microsomal proteins, diluted in 100 μl of ubiquitination assay buffer (1 M Tris/HCl, 500 mM MgCl 2 , 10 mM DTT, pH 8), using the E3Lite Customizable Ubiquitin Ligase kit (LifeSensors Inc., Malvern, PA), and divided into 3 aliquots. .. The first aliquot was incubated for 30 min at 37 °C, in the presence of 5 nM E1 activating enzyme provided by the kit, 100 nM E2 conjugating enzyme Ube2g2 (LifeSensors Inc.), 200 μM ATP, 6 mM human recombinant ubiquitin, to monitor the physiological ubiquitination of HMGCR in mock and p140Cap cells.

    Article Title: CMIP interacts with WT1 and targets it on the proteasome degradation pathway
    Article Snippet: The samples were analyzed by Western blot using mouse anti‐WT1 (F‐6, Santa Cruz) and rabbit anti‐CMIP antibodies. .. To test E3 ligase activity of CMIP, we used the E 3 ubiquitin ligase kit, according to the manufacturer's protocol (LifeSensors, Malvern, PA). ..

    Article Title: p140Cap modulates the mevalonate pathway decreasing cell migration and enhancing drug sensitivity in breast cancer cells.
    Article Snippet: .. The ubiquitination assay was performed on 100 μg of microsomal proteins, diluted in 100 μl of ubiquitination assay buffer (1 M Tris/HCl, 500mM MgCl2, 10 mM DTT, pH 8), using the E3Lite Customizable Ubiquitin Ligase kit (LifeSensors Inc., Malvern, PA), and divided into 3 aliquots. .. The first aliquot was incubated for 30min at 37 °C, in the presence of 5 nM E1 activating enzyme provided by the kit, 100 nM E2 conjugating enzyme Ube2g2 (LifeSensors Inc.), 200 μM ATP, 6 mM human recombinant ubiquitin, to monitor the physiological ubiquitination of HMGCR in mock and p140Cap cells.

    Article Title: Recognition and reprogramming of E3 ubiquitin ligase surfaces by α-helical peptides.
    Article Snippet: .. E3LITE Customizable Ubiquitin Ligase Kit (LifeSensors, UC101) was used to assess the autoubiquitination activity of HECT domain of WWP2 (WWP2HECT). ..

    Article Title: Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.
    Article Snippet: .. The activity of ICP22 as an E3 ubiquitin ligase was determined by an E3LITE Customizable Ubiquitin Ligase Kit (UC101; LifeSensors) according to the manufacturer’s protocol. ..

    Article Title: LncRNAs-directed PTEN enzymatic switch governs epithelial-mesenchymal transition.
    Article Snippet: PI3K activity in cells with indicated treatments was measured using PI3Kinase Activity ELISA: Pico (Echelon Biosciences). .. E3LITE MEX3C ubiquitin ligase assay and in vitro ubiquitination assay E3LITE Customizable Ubiquitin Ligase assay (LifeSensors) were performed according to the manufacture’s instruciton. .. In vitro ubiquitination assays were performed using an ubiquitin protein conjugation kit (BostonBiochem).

    Article Title: Coencapsulation of disulfiram and doxorubicin in liposomes strongly reverses multidrug resistance in breast cancer cells.
    Article Snippet: Disulfiram (DSF) is an inhibitor of P-glycoprotein (Pgp), the main obstacle limiting the success of doxorubicin (DOX), but it has poor solubility and stability.. With the aim to overcome these limitations we prepared liposomes coencapsulating DSF and DOX (LipoDSF-DOX).. Liposome stability, drugs release profile, effects on DOX cytotoxicity, Pgp activity and expression in breast cancer cells were evaluated.

    Activity Assay:

    Article Title: Recognition and reprogramming of E3 ubiquitin ligase surfaces by α-helical peptides
    Article Snippet: .. E3LITE Customizable Ubiquitin Ligase Kit (LifeSensors, UC101) was used to assess the autoubiquitination activity of HECT domain of WWP2 (WWP2 HECT ). ..

    Article Title: CMIP interacts with WT1 and targets it on the proteasome degradation pathway
    Article Snippet: The samples were analyzed by Western blot using mouse anti‐WT1 (F‐6, Santa Cruz) and rabbit anti‐CMIP antibodies. .. To test E3 ligase activity of CMIP, we used the E 3 ubiquitin ligase kit, according to the manufacturer's protocol (LifeSensors, Malvern, PA). ..

    Article Title: Recognition and reprogramming of E3 ubiquitin ligase surfaces by α-helical peptides.
    Article Snippet: .. E3LITE Customizable Ubiquitin Ligase Kit (LifeSensors, UC101) was used to assess the autoubiquitination activity of HECT domain of WWP2 (WWP2HECT). ..

    Article Title: Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.
    Article Snippet: .. The activity of ICP22 as an E3 ubiquitin ligase was determined by an E3LITE Customizable Ubiquitin Ligase Kit (UC101; LifeSensors) according to the manufacturer’s protocol. ..

    In Vitro:

    Article Title: LncRNAs-directed PTEN enzymatic switch governs epithelial-mesenchymal transition.
    Article Snippet: PI3K activity in cells with indicated treatments was measured using PI3Kinase Activity ELISA: Pico (Echelon Biosciences). .. E3LITE MEX3C ubiquitin ligase assay and in vitro ubiquitination assay E3LITE Customizable Ubiquitin Ligase assay (LifeSensors) were performed according to the manufacture’s instruciton. .. In vitro ubiquitination assays were performed using an ubiquitin protein conjugation kit (BostonBiochem).



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    Relative expression levels of GATA-4 and friend-of-GATA ( FOG )-2 according to <t>microarray</t> analyses. ( A – D ) GATA-4 and ( E – H ) FOG-2 . ( A and E ) MYCN -nonamplified ( n =218) vs MYCN -amplified ( n =32 for GATA-4 ; n =33 for FOG-2 ). ( B and F ) Localised stages ( n =153) vs stage 4 ( n =67) vs stage 4S ( n =31). ( C and G ) Patients below 1 year ( n =94) vs above 1 year ( n =157). ( D and H ) High-risk (HR; n =82) vs low-risk (LR; n =169) tumours according to the PAM classification. Expression values are given in log ratios (sample vs reference RNA). Boxes: median expression values (horizontal line) and twenty-fifth and seventy-fifth percentiles; whiskers: distances from the end of the box to the largest and smallest observed values that are <1.5 box lengths from either end of the box; open circles: outlying values; asterisks: extreme values. ** P <0.01; *** P <0.001 according to the nonparametric Mann–Whitney test.
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    Image Search Results


    Relative expression levels of GATA-4 and friend-of-GATA ( FOG )-2 according to microarray analyses. ( A – D ) GATA-4 and ( E – H ) FOG-2 . ( A and E ) MYCN -nonamplified ( n =218) vs MYCN -amplified ( n =32 for GATA-4 ; n =33 for FOG-2 ). ( B and F ) Localised stages ( n =153) vs stage 4 ( n =67) vs stage 4S ( n =31). ( C and G ) Patients below 1 year ( n =94) vs above 1 year ( n =157). ( D and H ) High-risk (HR; n =82) vs low-risk (LR; n =169) tumours according to the PAM classification. Expression values are given in log ratios (sample vs reference RNA). Boxes: median expression values (horizontal line) and twenty-fifth and seventy-fifth percentiles; whiskers: distances from the end of the box to the largest and smallest observed values that are <1.5 box lengths from either end of the box; open circles: outlying values; asterisks: extreme values. ** P <0.01; *** P <0.001 according to the nonparametric Mann–Whitney test.

    Journal: British Journal of Cancer

    Article Title: GATA factors in human neuroblastoma: distinctive expression patterns in clinical subtypes

    doi: 10.1038/sj.bjc.6605276

    Figure Lengend Snippet: Relative expression levels of GATA-4 and friend-of-GATA ( FOG )-2 according to microarray analyses. ( A – D ) GATA-4 and ( E – H ) FOG-2 . ( A and E ) MYCN -nonamplified ( n =218) vs MYCN -amplified ( n =32 for GATA-4 ; n =33 for FOG-2 ). ( B and F ) Localised stages ( n =153) vs stage 4 ( n =67) vs stage 4S ( n =31). ( C and G ) Patients below 1 year ( n =94) vs above 1 year ( n =157). ( D and H ) High-risk (HR; n =82) vs low-risk (LR; n =169) tumours according to the PAM classification. Expression values are given in log ratios (sample vs reference RNA). Boxes: median expression values (horizontal line) and twenty-fifth and seventy-fifth percentiles; whiskers: distances from the end of the box to the largest and smallest observed values that are <1.5 box lengths from either end of the box; open circles: outlying values; asterisks: extreme values. ** P <0.01; *** P <0.001 according to the nonparametric Mann–Whitney test.

    Article Snippet: In brief, 1 μ g of linearly amplified Cy3- and Cy5-labelled cRNA was hybridised together with 1 μ g of reverse colour Cy-labelled reference cRNA to a customised 11 kb oligonucleotide-microarray (Agilent Technologies, Waldbronn, Germany).

    Techniques: Expressing, Microarray, Amplification, MANN-WHITNEY

    Relative expression levels of GATA-2 and -3 according to microarray analyses. ( A – D ) GATA-2 and ( E – H ) GATA-3 . ( A and E ) MYCN -nonamplified ( n =218) vs MYCN -amplified ( n =33). ( B and F ) Localised stages ( n =153) vs stage 4 ( n =67) vs stage 4S ( n =31). ( C and G ) Patients below 1 year ( n =94) vs above 1 year ( n =157). ( D and H ) High-risk (HR; n =82) vs low-risk (LR; n =169) tumours according to the PAM classification. Expression values are given in log ratios (sample vs reference RNA). Box plots represent data as in . * P <0.05; ** P <0.01; *** P <0.001 according to the nonparametric Mann–Whitney test.

    Journal: British Journal of Cancer

    Article Title: GATA factors in human neuroblastoma: distinctive expression patterns in clinical subtypes

    doi: 10.1038/sj.bjc.6605276

    Figure Lengend Snippet: Relative expression levels of GATA-2 and -3 according to microarray analyses. ( A – D ) GATA-2 and ( E – H ) GATA-3 . ( A and E ) MYCN -nonamplified ( n =218) vs MYCN -amplified ( n =33). ( B and F ) Localised stages ( n =153) vs stage 4 ( n =67) vs stage 4S ( n =31). ( C and G ) Patients below 1 year ( n =94) vs above 1 year ( n =157). ( D and H ) High-risk (HR; n =82) vs low-risk (LR; n =169) tumours according to the PAM classification. Expression values are given in log ratios (sample vs reference RNA). Box plots represent data as in . * P <0.05; ** P <0.01; *** P <0.001 according to the nonparametric Mann–Whitney test.

    Article Snippet: In brief, 1 μ g of linearly amplified Cy3- and Cy5-labelled cRNA was hybridised together with 1 μ g of reverse colour Cy-labelled reference cRNA to a customised 11 kb oligonucleotide-microarray (Agilent Technologies, Waldbronn, Germany).

    Techniques: Expressing, Microarray, Amplification, MANN-WHITNEY