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custom-made microarray chip  (Thermo Fisher)


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    Structured Review

    Thermo Fisher custom-made microarray chip
    Custom Made Microarray Chip, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom-made+microarray+chip/Custom+5'+Fluorescent+Labeled%2FUnlabeled+Pairs/pmc06744074-132-14-16
    Average 90 stars, based on 1 article reviews
    custom-made microarray chip - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Compositions and methods of treating Facioscapulohumeral muscular dystrophy
    Article Snippet: RNA isolation was performed using Direct-zol-96 RNA isolation kit (Zymo) according to the manufacturer's instructions. .. 100-500 ng of purified RNA was converted to cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using SimpliAmp Thermal Cycler (Applied Biosystems). cDNA was analyzed by qPCR using TaqMan Fast Universal Master Mix II (Thermo Fisher) and TaqMan probes (Thermo Fisher) in duplicates, using QuantStudio 6 or 7 Flex Real-Time PCR instruments (Applied Biosystems). .. Data were analyzed by QuantStudioTM Real-Time PCR Software v1.3 (Applied Biosystems).

    Reverse Transcription:

    Article Title: Compositions and methods of treating Facioscapulohumeral muscular dystrophy
    Article Snippet: RNA isolation was performed using Direct-zol-96 RNA isolation kit (Zymo) according to the manufacturer's instructions. .. 100-500 ng of purified RNA was converted to cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using SimpliAmp Thermal Cycler (Applied Biosystems). cDNA was analyzed by qPCR using TaqMan Fast Universal Master Mix II (Thermo Fisher) and TaqMan probes (Thermo Fisher) in duplicates, using QuantStudio 6 or 7 Flex Real-Time PCR instruments (Applied Biosystems). .. Data were analyzed by QuantStudioTM Real-Time PCR Software v1.3 (Applied Biosystems).

    Real-time Polymerase Chain Reaction:

    Article Title: Compositions and methods of treating Facioscapulohumeral muscular dystrophy
    Article Snippet: RNA isolation was performed using Direct-zol-96 RNA isolation kit (Zymo) according to the manufacturer's instructions. .. 100-500 ng of purified RNA was converted to cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using SimpliAmp Thermal Cycler (Applied Biosystems). cDNA was analyzed by qPCR using TaqMan Fast Universal Master Mix II (Thermo Fisher) and TaqMan probes (Thermo Fisher) in duplicates, using QuantStudio 6 or 7 Flex Real-Time PCR instruments (Applied Biosystems). .. Data were analyzed by QuantStudioTM Real-Time PCR Software v1.3 (Applied Biosystems).

    Article Title: EcCas6e-based antisense crRNA for gene repression and RNA editing in microorganisms
    Article Snippet: .. SYBR Green Real-Time PCR Master Mix (TaKaRa) with custom primers was used for real-time quantitative PCR (Applied Biosystems). ..

    Article Title: LIM-domain-only 4 (LMO4) enhances CD8 + T-cell stemness and tumor rejection by boosting IL-21-STAT3 signaling.
    Article Snippet: Quantitative PCR The Isolation of RNA was performed using the RNeasy Mini Kit (Qiagen). .. Complementary DNA (Applied Biosystems) was obtained by PCR. qPCR was done using primers from Applied Biosystems and a Quant Studio 3 (Applied Biosystems) using PowerUp SYBR Green Master Mix (Applied Biosystems). ..

    Clone Assay:

    Article Title: Deciphering the genetics and mechanisms of predisposition to multiple myeloma
    Article Snippet: To delete the rs4273077 and 4792800-harboring regions in TNFRSF13B , we used dual-sgRNA CRISPR/Cas9 genome editing. sgRNA pairs were selected using CRISPOR (crispor.org; Supplementary Data ) and cloned into the pSpCas9(BB)-2A-GFP PX458 vector (Addgene no. 48138). .. Cloned sgRNA pairs were co-transfected (ThermoFisher Neon) into Raji cells. ..

    SYBR Green Assay:

    Article Title: EcCas6e-based antisense crRNA for gene repression and RNA editing in microorganisms
    Article Snippet: .. SYBR Green Real-Time PCR Master Mix (TaKaRa) with custom primers was used for real-time quantitative PCR (Applied Biosystems). ..

    Article Title: LIM-domain-only 4 (LMO4) enhances CD8 + T-cell stemness and tumor rejection by boosting IL-21-STAT3 signaling.
    Article Snippet: Quantitative PCR The Isolation of RNA was performed using the RNeasy Mini Kit (Qiagen). .. Complementary DNA (Applied Biosystems) was obtained by PCR. qPCR was done using primers from Applied Biosystems and a Quant Studio 3 (Applied Biosystems) using PowerUp SYBR Green Master Mix (Applied Biosystems). ..

    Amplification:

    Article Title: Synonymous codon substitutions modulate transcription and translation of a divergent upstream gene by modulating antisense RNA production.
    Article Snippet: A central goal of synthetic biology is to express protein-encoding genes at desired levels, but the full repertoire of DNA sequence elements that determine the transcription and translation level remains poorly understood, particularly for sequence elements that lie far from the canonical transcription start site.. Here, we show that synonymous codon changes far within a coding sequence can alter both transcription and translation of a neighboring gene.. These results highlight a previously underappreciated selective pressure on codon usage in bacteria, where protein-coding sequences are often packed close together.

    Article Title: Association of oral fungal profiles with health status and bacterial composition in elderly adults receiving community support and home care service.
    Article Snippet: Fungi compose a minority but a common component of normal oral microbiota and contribute to oral and systemic health by interacting with bacterial inhabitants.. This study investigated the relationship of oral fungal profiles to health status and bacterial profiles of 159 elderly adults receiving community support and home care services.. Fungal and bacterial densities and compositions were determined based on the fungal ribosomal internal transcribed spacer region and bacterial 16S rRNA gene amplicon analyses, respectively.

    Polymerase Chain Reaction:

    Article Title: Synonymous codon substitutions modulate transcription and translation of a divergent upstream gene by modulating antisense RNA production.
    Article Snippet: A central goal of synthetic biology is to express protein-encoding genes at desired levels, but the full repertoire of DNA sequence elements that determine the transcription and translation level remains poorly understood, particularly for sequence elements that lie far from the canonical transcription start site.. Here, we show that synonymous codon changes far within a coding sequence can alter both transcription and translation of a neighboring gene.. These results highlight a previously underappreciated selective pressure on codon usage in bacteria, where protein-coding sequences are often packed close together.

    Article Title: LIM-domain-only 4 (LMO4) enhances CD8 + T-cell stemness and tumor rejection by boosting IL-21-STAT3 signaling.
    Article Snippet: Quantitative PCR The Isolation of RNA was performed using the RNeasy Mini Kit (Qiagen). .. Complementary DNA (Applied Biosystems) was obtained by PCR. qPCR was done using primers from Applied Biosystems and a Quant Studio 3 (Applied Biosystems) using PowerUp SYBR Green Master Mix (Applied Biosystems). ..

    Nested PCR:

    Article Title: Synonymous codon substitutions modulate transcription and translation of a divergent upstream gene by modulating antisense RNA production.
    Article Snippet: A central goal of synthetic biology is to express protein-encoding genes at desired levels, but the full repertoire of DNA sequence elements that determine the transcription and translation level remains poorly understood, particularly for sequence elements that lie far from the canonical transcription start site.. Here, we show that synonymous codon changes far within a coding sequence can alter both transcription and translation of a neighboring gene.. These results highlight a previously underappreciated selective pressure on codon usage in bacteria, where protein-coding sequences are often packed close together.

    other:

    Article Title: A glia-enriched stem cell 3D model of the human brain mimics the glial-immune neurodegenerative phenotypes of multiple sclerosis.
    Article Snippet: After 15 days in vitro, theHEK293Tcellswere harvested, then theDNAwas extracted and analyzed by a real-timequantitative PCR (QuantStudio3, ThermoFischer), using the following primers: GAPDH #Hs00483111_cn as housekeeping gene; for the sequence of LV we used Probe LV (50-ATCTCTCTCCTTCTAGCCTC-30) and Primer FW (50-TACTGACGCTCTCGCACC-30), Primer REV (50-TCTCGACGCAGGACTCG-30) (ThermoFisher).

    Article Title: HMGB1 restores a dynamic chromatin environment in the presence of linker histone by deforming nucleosomal DNA
    Article Snippet: To generate labeled array DNA, the sticky end generated by XhoI digest was filled in using Klenow polymerase (Invitrogen) with an Alexa Fluor 647-labeled dCTP (Invitrogen) along with unlabeled dATP, dTTP, and dGTP according to manufacturer’s instructions.

    Sequencing:

    Article Title: Association of oral fungal profiles with health status and bacterial composition in elderly adults receiving community support and home care service.
    Article Snippet: Fungi compose a minority but a common component of normal oral microbiota and contribute to oral and systemic health by interacting with bacterial inhabitants.. This study investigated the relationship of oral fungal profiles to health status and bacterial profiles of 159 elderly adults receiving community support and home care services.. Fungal and bacterial densities and compositions were determined based on the fungal ribosomal internal transcribed spacer region and bacterial 16S rRNA gene amplicon analyses, respectively.



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    Image Search Results


    Table 3

    Journal: Infection and Immunity

    Article Title: Control of Bacterial Virulence by the RalR Regulator of the Rabbit-Specific Enteropathogenic Escherichia coli Strain E22

    doi: 10.1128/IAI.00710-13

    Figure Lengend Snippet: Table 3

    Article Snippet: Total RNA from both the control strain, E22Δ2(pACYC184) (RalR − ), and the ralR -complemented test strain, E22Δ2(+pYS23) (RalR + ), were fluorescently labeled and hybridized to a custom-made microarray chip (Agilent Technologies) covering all predicted ORFs of the E22 genome.

    Techniques: Microarray