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CombiMatrix 12k combimatrix oligonucleotide array
12k Combimatrix Oligonucleotide Array, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-made+microarray+chip/12k+combimatrix+oligonucleotide+array/pmc03271758-176-18-20
Average 90 stars, based on 1 article reviews
12k combimatrix oligonucleotide array - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Microarray:

Article Title: Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae
Article Snippet: .. Here, we describe the use of a custom 12K CombiMatrix ElectraSenseTM oligonucleotide microarray format for assessing global gene expression profiles in Neisseria spp. ..

Article Title: Microarray having a base cleavable linker
Article Snippet: .. An example of an electrode microarray is a CombiMatrix CustomArrayTM 12K, which has over 12,000 electrodes and an electrode density of approximately 17,778 electrodes per square centimeter. ..

Article Title: Multiplex microarray of serially deposited biomolecules on a microarray
Article Snippet: .. Serial Attachment of Proteins on 12K CombiMatrix Microarray In an embodiment, a method for serial construction of a protein microarray comprises: (a) blocking a microarray with a blocking protein, wherein the blocking protein is Casein or Bovine Calf Serum or a combination thereof; (b) washing the microarray to remove the blocking solution, wherein the step of washing comprises washing three times with PBS/Tween, three times with PBS, and three times with the electrolyte used for the polymerization of pyrrole; (c) electropolymerizing pyrrole for 5 seconds at 1.5 V in Sodium Phosphate on an electrode or set of electrodes that have been predetermined; (d) washing the microarray 3 times with PBS; (e) diluting a protein solution to about 1 ug/ml; (f) exposing the microarray to the protein solution for about 5 to 30 minutes; (g) washing the microarray three times with PBS/tween; (h) blocking the microarray with a blocking solution, wherein the blocking solution is saturated Casein and the time of blocking is about 2-5 minutes; and (i) repeating steps (b) through (h) for each protein to be attached to the microarray. ..

Article Title: Expression profiling of genes involved in the formation of aroma in two peach genotypes.
Article Snippet: The family Rosaceae includes many important fruit crops, such as peach, almond, apricot, plum and cherry (belonging to the Prunus genus), as well as blackberry, blueberry, pear, apple, raspberry, strawberry and various ornamentals.. Worldwide peach production is 20.3 million t per annum and is second only to apple among fruits grown in temperate regions (FAOSTAT 2009, http://faostat.fao.org).. For industrial and commercial reasons, fruit is harvested well before the physiological ripe stage, and thus perceived by the consumer as of poor value for money.

Article Title: Microarray analysis of differentially expressed mRNAs and miRNAs in young leaves of sorghum under dry-down conditions.
Article Snippet: Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency.. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a “dry-down” experiment under controlled conditions.. The incoming stress was monitored by determining the water potential available for 4-leaf-old plants.

Article Title: Serially deposited biomolecules
Article Snippet: .. Serial Attachment of Proteins on 12K CombiMatrix Microarray In an embodiment, a method for serial construction of a protein microarray comprises: (a) blocking a microarray with a blocking protein, wherein the blocking protein is Casein or Bovine Calf Serum or a combination thereof; (b) washing the microarray to remove the blocking solution, wherein the step of washing comprises washing three times with PBS/Tween, three times with PBS, and three times with the electrolyte used for the polymerization of pyrrole; (c) electropolymerizing pyrrole for 5 seconds at 1.5 V in Sodium Phosphate on an electrode or set of electrodes that have been predetermined; (d) washing the microarray 3 times with PBS; (e) diluting a protein solution to about 1 μg/ml; (f) exposing the microarray to the protein solution for about 5 to 30 minutes; (g) washing the microarray three times with PBS/tween; (h) blocking the microarray with a blocking solution, wherein the blocking solution is saturated Casein and the time of blocking is about 2-5 minutes; and (i) repeating steps (b) through (h) for each protein to be attached to the microarray. ..

Gene Expression:

Article Title: Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae
Article Snippet: .. Here, we describe the use of a custom 12K CombiMatrix ElectraSenseTM oligonucleotide microarray format for assessing global gene expression profiles in Neisseria spp. ..

Blocking Assay:

Article Title: Multiplex microarray of serially deposited biomolecules on a microarray
Article Snippet: .. Serial Attachment of Proteins on 12K CombiMatrix Microarray In an embodiment, a method for serial construction of a protein microarray comprises: (a) blocking a microarray with a blocking protein, wherein the blocking protein is Casein or Bovine Calf Serum or a combination thereof; (b) washing the microarray to remove the blocking solution, wherein the step of washing comprises washing three times with PBS/Tween, three times with PBS, and three times with the electrolyte used for the polymerization of pyrrole; (c) electropolymerizing pyrrole for 5 seconds at 1.5 V in Sodium Phosphate on an electrode or set of electrodes that have been predetermined; (d) washing the microarray 3 times with PBS; (e) diluting a protein solution to about 1 ug/ml; (f) exposing the microarray to the protein solution for about 5 to 30 minutes; (g) washing the microarray three times with PBS/tween; (h) blocking the microarray with a blocking solution, wherein the blocking solution is saturated Casein and the time of blocking is about 2-5 minutes; and (i) repeating steps (b) through (h) for each protein to be attached to the microarray. ..

Article Title: Serially deposited biomolecules
Article Snippet: .. Serial Attachment of Proteins on 12K CombiMatrix Microarray In an embodiment, a method for serial construction of a protein microarray comprises: (a) blocking a microarray with a blocking protein, wherein the blocking protein is Casein or Bovine Calf Serum or a combination thereof; (b) washing the microarray to remove the blocking solution, wherein the step of washing comprises washing three times with PBS/Tween, three times with PBS, and three times with the electrolyte used for the polymerization of pyrrole; (c) electropolymerizing pyrrole for 5 seconds at 1.5 V in Sodium Phosphate on an electrode or set of electrodes that have been predetermined; (d) washing the microarray 3 times with PBS; (e) diluting a protein solution to about 1 μg/ml; (f) exposing the microarray to the protein solution for about 5 to 30 minutes; (g) washing the microarray three times with PBS/tween; (h) blocking the microarray with a blocking solution, wherein the blocking solution is saturated Casein and the time of blocking is about 2-5 minutes; and (i) repeating steps (b) through (h) for each protein to be attached to the microarray. ..

Expressing:

Article Title: Microarray analysis of differentially expressed mRNAs and miRNAs in young leaves of sorghum under dry-down conditions.
Article Snippet: Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency.. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a “dry-down” experiment under controlled conditions.. The incoming stress was monitored by determining the water potential available for 4-leaf-old plants.

Hybridization:

Article Title: Microarray analysis of differentially expressed mRNAs and miRNAs in young leaves of sorghum under dry-down conditions.
Article Snippet: Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency.. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a “dry-down” experiment under controlled conditions.. The incoming stress was monitored by determining the water potential available for 4-leaf-old plants.

other:

Article Title: Solanum torvum responses to the root-knot nematode Meloidogyne incognita
Article Snippet: This also allowed to contain the number of probes in the chip to maximum 30,000, consistent with a triplicate probe layout in the 90k-features Combimatrix chip design.



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Image Search Results


Table 3

Journal: Infection and Immunity

Article Title: Control of Bacterial Virulence by the RalR Regulator of the Rabbit-Specific Enteropathogenic Escherichia coli Strain E22

doi: 10.1128/IAI.00710-13

Figure Lengend Snippet: Table 3

Article Snippet: Total RNA from both the control strain, E22Δ2(pACYC184) (RalR − ), and the ralR -complemented test strain, E22Δ2(+pYS23) (RalR + ), were fluorescently labeled and hybridized to a custom-made microarray chip (Agilent Technologies) covering all predicted ORFs of the E22 genome.

Techniques: Microarray