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sureprint g3 4×44k custom oligonucleotide microarrays (design number 021966, 021362)  (Agilent technologies)


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    Agilent technologies sureprint g3 4×44k custom oligonucleotide microarrays (design number 021966, 021362)
    Sureprint G3 4×44k Custom Oligonucleotide Microarrays (Design Number 021966, 021362), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom-designed+oligonucleotide+microarray/pmc10500703-584-14-13
    Average 90 stars, based on 1 article reviews
    sureprint g3 4×44k custom oligonucleotide microarrays (design number 021966, 021362) - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Radiogenomic profiling of prostate tumors prior to external beam radiotherapy converges on a transcriptomic signature of TGF-β activity driving tumor recurrence
    Article Snippet: Gene level summaries from Agilent 4×44K microarrays of LNCaP and HPS-19I cultures [ ] were downloaded from GEO (Accession ID GSE51624).

    Article Title: The Arabidopsis Deubiquitylase OTU5 Suppresses Flowering by Histone Modification-Mediated Activation of the Major Flowering Repressors FLC , MAF4 , and MAF5 .
    Article Snippet: The fragmented cRNA was then pooled and hybridized to Arabidopsis (V4) 4×44K Microarray (Agilent Technologies) at 65 ◦C for 17 h. After the washing and drying steps, the microarrays were scanned with an Agilent microarray scanner (Agilent Technologies) at 535 nm for Cy3.

    Article Title: Histone deacetylation primes self-propagation of heterochromatin domains to promote epigenetic inheritance
    Article Snippet: Equal amounts of Cy5- and Cy3-labeled DNA were mixed and hybridized to 4×44K Agilent custom 60-mer oligonucleotide array according to Agilent’s protocol.

    Article Title: Staphylococcal protein A modulates inflammation by inducing interferon signaling in human nasal epithelial cells.
    Article Snippet: For RNA profiling, the Agilent Whole Mouse Genome Microarray 4×44K was used according to the manufacturer’s instruction with 100 ng RNA (RNA integrity number (RIN)> = 7).

    Article Title: c-Myc inhibits LAPTM5 expression in B-cell lymphomas.
    Article Snippet: Myc is a pivotal protooncogenic transcription factor that contributes to the development of almost all Burkitt’s lymphomas and about one-third of diffuse large B-cell lymphomas.. How B-cells sustain their uncontrolled proliferation due to high Myc is not yet well defined.. Here, we found that Myc trans-represses the expression of murine LAPTM5, a gene coding a lysosomeassociated protein, by binding to two E-boxes in the LAPTM5 promoter.

    Microarray:

    Article Title: Proteogenomic analysis reveals adaptive strategies to alleviate the consequences of aneuploidy in cancer
    Article Snippet: Genome-wide comparative genomic hybridization of cell lines (Supplementary Table 2) was carried out by IMGM laboratories (Martinsried, Germany). .. Agilent Human Genome CGH Microarrays (4×44K format) and SurePrint G3 Human CGH Microarray (4×180K format) were used in combination with a Two-Color based hybridization protocol with a commercially available reference gDNA. .. Signals on the microarrays were extracted using the Agilent DNA Microarray Scanner.

    Article Title: MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
    Article Snippet: The cRNA probe was generated from total RNA (500 ng) with the Low RNA Input linear amplification & Labeling kit (Agilent Technologies, Santa Clara, CA, USA). .. Cy3-labeled cRNA (1.65 μg) was then fragmented and relative expression was measured by hybridization to 4×44K whole human oligo microarray (Agilent). ..

    Hybridization:

    Article Title: Proteogenomic analysis reveals adaptive strategies to alleviate the consequences of aneuploidy in cancer
    Article Snippet: Genome-wide comparative genomic hybridization of cell lines (Supplementary Table 2) was carried out by IMGM laboratories (Martinsried, Germany). .. Agilent Human Genome CGH Microarrays (4×44K format) and SurePrint G3 Human CGH Microarray (4×180K format) were used in combination with a Two-Color based hybridization protocol with a commercially available reference gDNA. .. Signals on the microarrays were extracted using the Agilent DNA Microarray Scanner.

    Article Title: MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
    Article Snippet: The cRNA probe was generated from total RNA (500 ng) with the Low RNA Input linear amplification & Labeling kit (Agilent Technologies, Santa Clara, CA, USA). .. Cy3-labeled cRNA (1.65 μg) was then fragmented and relative expression was measured by hybridization to 4×44K whole human oligo microarray (Agilent). ..

    Article Title: 1,3-Diarylpyrazolyl-acylsulfonamides as potent anti-tuberculosis agents targeting cell wall biosynthesis in Mycobacterium tuberculosis
    Article Snippet: Fluorescent cDNA was analyzed with the Nanodrop ND-1000. .. Equal amounts (0.7 μg) of Cy3- and Cy5-labeled cDNA were hybridized to the Agilent SurePrint G3 4×44K custom oligonucleotide microarrays (design number 021966, 021362) in the TECAN HS Pro 4800 hybridization station. .. Hybridization was performed using Agilent 2x Gene expression hybridization HI-RPM buffer, and 10x Blocking Reagent at 65 °C for 17 h. Arrays were washed with Agilent Gene Expression Wash Buffer 1 at room temperature and Gene Expression Wash Buffer 2 at 37 °C.

    Expressing:

    Article Title: MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
    Article Snippet: The cRNA probe was generated from total RNA (500 ng) with the Low RNA Input linear amplification & Labeling kit (Agilent Technologies, Santa Clara, CA, USA). .. Cy3-labeled cRNA (1.65 μg) was then fragmented and relative expression was measured by hybridization to 4×44K whole human oligo microarray (Agilent). ..



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    The rectangular boxes represent the entire transcriptomes of C. elegans and P. pacificus genes assayed on our <t>microarrays,</t> and their area of overlap represents the set of 6,126 1∶1 orthologs present on microarrays of both the nematodes. The ovals represent the fraction of differentially expressed genes in each of the subsets. For the 1∶1 orthologs, we assessed the significance of overlap between the genes differentially expressed in response to a given pathogen using a 2×2 Fisher's exact test. Differences or similarities in survival characteristics of the two nematodes when exposed to the same bacteria are reflected in their respective transcriptional responses. (A) On B. thuringiensis DB27, which is highly lethal to C. elegans but not to P. pacificus , only 23 genes are common between the respective expression profiles of the two nematodes, this overlap being statistically not significant. (B) Similarly, S. aureus is more lethal to C. elegans than P. pacificus , and the overlap between the corresponding expression profiles is limited to just 6 orthologs. Although this overlap is statistically significant (p-value = 0.0002), the extent of overlap is too small to be biologically significant. (C) S. marcescens is lethal to both the nematodes and the extent and significance overlap between the orthologs differentially expressed in the corresponding expression profiles is also relatively high (443 common orthologs, p-value = 7.71E–37). (D) On the pathogen X. nematophila , the observed overlap between the expression profiles in the two nematodes is even higher, with 2,093 orthologs regulated in both nematodes (p-value = 6.92E–134).
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    Image Search Results


    The relative transcriptional level of 10 selected genes determined by DNA microarray and qRT-PCR analyses were log 2 transformed, and the values were plotted against each other to evaluate their correlation. The genes analysed by qRT-PCR are listed in .

    Journal: PLoS ONE

    Article Title: Two Spx Regulators Modulate Stress Tolerance and Virulence in Streptococcus suis Serotype 2

    doi: 10.1371/journal.pone.0108197

    Figure Lengend Snippet: The relative transcriptional level of 10 selected genes determined by DNA microarray and qRT-PCR analyses were log 2 transformed, and the values were plotted against each other to evaluate their correlation. The genes analysed by qRT-PCR are listed in .

    Article Snippet: DNA microarray analysis was performed using an Agilent custom-designed oligonucleotide microarray.

    Techniques: Microarray, Quantitative RT-PCR, Transformation Assay

    Expression ratios of genes involved in oxidative stress response and virulence in the mutant strains relative to the WT strain by  microarray  analysis <xref ref-type= a ." width="100%" height="100%">

    Journal: PLoS ONE

    Article Title: Two Spx Regulators Modulate Stress Tolerance and Virulence in Streptococcus suis Serotype 2

    doi: 10.1371/journal.pone.0108197

    Figure Lengend Snippet: Expression ratios of genes involved in oxidative stress response and virulence in the mutant strains relative to the WT strain by microarray analysis a .

    Article Snippet: DNA microarray analysis was performed using an Agilent custom-designed oligonucleotide microarray.

    Techniques: Expressing, Mutagenesis, Microarray

    Array findings from prenatal single nucleotide polymorphism microarray on amniocytes (estimated 60% mosaicism). Image courtesy of LabCorp (Burlington, North Carolina, United States).

    Journal: Journal of Pediatric Genetics

    Article Title: Mosaic Trisomy 16 Associated with Left Lung Agenesis, Abnormal Left Arm, and Right Pulmonary Artery Stenosis: Expanding the Phenotype and Review of the Literature

    doi: 10.1055/s-0040-1721136

    Figure Lengend Snippet: Array findings from prenatal single nucleotide polymorphism microarray on amniocytes (estimated 60% mosaicism). Image courtesy of LabCorp (Burlington, North Carolina, United States).

    Article Snippet: The CMA on foreskin was performed on a custom designed oligonucleotide microarray (GenomeDx v5).

    Techniques: Microarray

    Array findings from postnatal single nucleotide polymorphism microarray on foreskin tissue (estimated 25–40% mosaicism). Image courtesy of GeneDx (Gaithersburg, Maryland, United States).

    Journal: Journal of Pediatric Genetics

    Article Title: Mosaic Trisomy 16 Associated with Left Lung Agenesis, Abnormal Left Arm, and Right Pulmonary Artery Stenosis: Expanding the Phenotype and Review of the Literature

    doi: 10.1055/s-0040-1721136

    Figure Lengend Snippet: Array findings from postnatal single nucleotide polymorphism microarray on foreskin tissue (estimated 25–40% mosaicism). Image courtesy of GeneDx (Gaithersburg, Maryland, United States).

    Article Snippet: The CMA on foreskin was performed on a custom designed oligonucleotide microarray (GenomeDx v5).

    Techniques: Microarray

    The rectangular boxes represent the entire transcriptomes of C. elegans and P. pacificus genes assayed on our microarrays, and their area of overlap represents the set of 6,126 1∶1 orthologs present on microarrays of both the nematodes. The ovals represent the fraction of differentially expressed genes in each of the subsets. For the 1∶1 orthologs, we assessed the significance of overlap between the genes differentially expressed in response to a given pathogen using a 2×2 Fisher's exact test. Differences or similarities in survival characteristics of the two nematodes when exposed to the same bacteria are reflected in their respective transcriptional responses. (A) On B. thuringiensis DB27, which is highly lethal to C. elegans but not to P. pacificus , only 23 genes are common between the respective expression profiles of the two nematodes, this overlap being statistically not significant. (B) Similarly, S. aureus is more lethal to C. elegans than P. pacificus , and the overlap between the corresponding expression profiles is limited to just 6 orthologs. Although this overlap is statistically significant (p-value = 0.0002), the extent of overlap is too small to be biologically significant. (C) S. marcescens is lethal to both the nematodes and the extent and significance overlap between the orthologs differentially expressed in the corresponding expression profiles is also relatively high (443 common orthologs, p-value = 7.71E–37). (D) On the pathogen X. nematophila , the observed overlap between the expression profiles in the two nematodes is even higher, with 2,093 orthologs regulated in both nematodes (p-value = 6.92E–134).

    Journal: PLoS ONE

    Article Title: System Wide Analysis of the Evolution of Innate Immunity in the Nematode Model Species Caenorhabditis elegans and Pristionchus pacificus

    doi: 10.1371/journal.pone.0044255

    Figure Lengend Snippet: The rectangular boxes represent the entire transcriptomes of C. elegans and P. pacificus genes assayed on our microarrays, and their area of overlap represents the set of 6,126 1∶1 orthologs present on microarrays of both the nematodes. The ovals represent the fraction of differentially expressed genes in each of the subsets. For the 1∶1 orthologs, we assessed the significance of overlap between the genes differentially expressed in response to a given pathogen using a 2×2 Fisher's exact test. Differences or similarities in survival characteristics of the two nematodes when exposed to the same bacteria are reflected in their respective transcriptional responses. (A) On B. thuringiensis DB27, which is highly lethal to C. elegans but not to P. pacificus , only 23 genes are common between the respective expression profiles of the two nematodes, this overlap being statistically not significant. (B) Similarly, S. aureus is more lethal to C. elegans than P. pacificus , and the overlap between the corresponding expression profiles is limited to just 6 orthologs. Although this overlap is statistically significant (p-value = 0.0002), the extent of overlap is too small to be biologically significant. (C) S. marcescens is lethal to both the nematodes and the extent and significance overlap between the orthologs differentially expressed in the corresponding expression profiles is also relatively high (443 common orthologs, p-value = 7.71E–37). (D) On the pathogen X. nematophila , the observed overlap between the expression profiles in the two nematodes is even higher, with 2,093 orthologs regulated in both nematodes (p-value = 6.92E–134).

    Article Snippet: For P. pacificus experiments, we used our custom designed oligonucleotide microarrays manufactured by Agilent Technologies, which contain ∼93,000 unique probes for the ∼23,000 P. pacificus predicted genes (NCBI GEO accession GPL14372, see for design details of custom microarrays).

    Techniques: Expressing