custom built thorlabs epicerna fluorescence microscope (Thorlabs)
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Custom Built Thorlabs Epicerna Fluorescence Microscope, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+microscope/built+custom+epicerna+fluorescence+microscope+thorlabs/pmc13269207-107-6-7
Average 86 stars, based on 1 article reviews
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![( A ) Illustration of the single molecule imaging assay, which combines microfluidics, surface immobilization and <t>TIRF</t> microscopy. ( B ) Typical field-of-view showing individual flow-aligned DNA strands (green) with MpARF2 nanoclusters (magenta) bound to the DNA templates. [MpARF2] = 1 nM. Scale bar: 5 µm. Inset: filtered Region Of Interest (ROI). ( C ) Kymograph of a nanocluster binding to DNA, illustrating the high temporal stability of a nanocluster once bound, with virtually no lateral movement. ( D ) Binding isotherm of nanoclusters, determined from the area fraction of protein covering the DNA, ϕ . Inset: binary-filtered ROIs used in coverage analysis. ( E ) Spatial distribution of binding events along individual DNA strands (solid) compared to the predicted IR7 positions (dashed). Inset: enrichment of protein on nonspecific (green) and IR7-containing (orange) DNA templates, measured as the ratio of protein intensity on DNA compared to the surrounding background. *** P <0.001; Welch’s t-test.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_32/10__1101_slash_2025__11__05__686732/10__1101_slash_2025__11__05__686732___F4.large.jpg)