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custom built zeiss axio observer 3 microscope stage  (Carl Zeiss)


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    Structured Review

    Carl Zeiss custom built zeiss axio observer 3 microscope stage
    Custom Built Zeiss Axio Observer 3 Microscope Stage, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom-built+microscope/Microscope+stand+Axio+Observer+3/pmc13049531-392-7-8
    Average 99 stars, based on 1540 article reviews
    custom built zeiss axio observer 3 microscope stage - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Bisphenol A induces hepatotoxicity via a LAMP3-dependent impairment of mitophagic processing and reduced PI3K-AKT-mTOR signaling.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Copper homeostasis dysregulation promotes malignant transformation of renal tubular epithelial cells via the ROS/NF-κB/AID signaling axis
    Article Snippet: The cells were then cultured in serum-free medium, and images were captured at 0 and 24 h under a microscope (Axio Observer 3, Zeiss, Germany).

    Article Title: EMSCs-derived exosomes rescue p75NTR −/− -mediated osteogenesis deficiency via PI3K/Akt/ERK1/2 signaling pathways
    Article Snippet: The cellular localization of DiI-labeled exosomes within EMSCs was observed using a fluorescence microscope (Axio Observer3, Zeiss, Germany).

    Brightfield:

    Article Title: BIOORTHOGONAL TUNING OF HYDROGEL STIFFNESS PROMOTES ZONAL REDIFFERENTIATION OF PASSAGED CHONDROCYTES.
    Article Snippet: Glycosaminoglycans were visualized using Alcian blue (pH 1.0, StatLab, KTABP1) and Toluidine blue (ThermoFisher, 348600050) staining, while collagen distribution was assessed using the Picro-Sirius Red Stain Kit (StatLab, KTPSRPT) according to manufacturer’s instructions. .. Sections were cover slipped using Acrymount Plus Mounting Media (StatLab) and brightfield microscopy images were captured using a Zeiss Axio Observer microscope (Zeiss) with a 2.5× objective (NA 0.12). .. Statistical analyses were performed using GraphPad Prism (GraphPad Software Inc., Boston, MA, USA).

    Microscopy:

    Article Title: BIOORTHOGONAL TUNING OF HYDROGEL STIFFNESS PROMOTES ZONAL REDIFFERENTIATION OF PASSAGED CHONDROCYTES.
    Article Snippet: Glycosaminoglycans were visualized using Alcian blue (pH 1.0, StatLab, KTABP1) and Toluidine blue (ThermoFisher, 348600050) staining, while collagen distribution was assessed using the Picro-Sirius Red Stain Kit (StatLab, KTPSRPT) according to manufacturer’s instructions. .. Sections were cover slipped using Acrymount Plus Mounting Media (StatLab) and brightfield microscopy images were captured using a Zeiss Axio Observer microscope (Zeiss) with a 2.5× objective (NA 0.12). .. Statistical analyses were performed using GraphPad Prism (GraphPad Software Inc., Boston, MA, USA).

    Article Title: Mitochondrial DNA damage elicits PINK1 upregulation in pontine cholinergic neurons in Parkinson's disease.
    Article Snippet: .. laser-capture microdissection (LCM) system on an AxioObserver 3 microscope (Zeiss, Germany) with motorized stage and CCD camera (AxioCam, Zeiss). .. Isolated 4 neurons were collected into sterile polymerase chain reaction (PCR) tube caps (STARLAB, UK) 5 containing 15 μl lysis buffer (1% Tween-20, Tris-buffered saline (TBS), nuclease-free water, and 6 proteinase K (Invitrogen, USA).

    Article Title: Investigating Precursor Spatial Organization in Osteoclastogenesis Using DNA-Directed Cell Patterning.
    Article Snippet: .. Stained cells were then imaged using a Zeiss Axio Observer microscope. ..

    Article Title: Physiological elasticity and topography modulate in vitro prostate cancer cell behavior
    Article Snippet: .. Microscopy analysis was performed on a Zeiss Axio Observer microscope equipped with a fluorescence Colibri5 light source, a second LED light source, an Axiocam 503 Mono, an Apotome 3 and the corresponding Zen 3.8 software (RRID: SCR_013672 ). ..

    Laser Capture Microdissection:

    Article Title: Mitochondrial DNA damage elicits PINK1 upregulation in pontine cholinergic neurons in Parkinson's disease.
    Article Snippet: .. laser-capture microdissection (LCM) system on an AxioObserver 3 microscope (Zeiss, Germany) with motorized stage and CCD camera (AxioCam, Zeiss). .. Isolated 4 neurons were collected into sterile polymerase chain reaction (PCR) tube caps (STARLAB, UK) 5 containing 15 μl lysis buffer (1% Tween-20, Tris-buffered saline (TBS), nuclease-free water, and 6 proteinase K (Invitrogen, USA).

    Staining:

    Article Title: Investigating Precursor Spatial Organization in Osteoclastogenesis Using DNA-Directed Cell Patterning.
    Article Snippet: .. Stained cells were then imaged using a Zeiss Axio Observer microscope. ..

    Analysis:

    Article Title: Physiological elasticity and topography modulate in vitro prostate cancer cell behavior
    Article Snippet: .. Microscopy analysis was performed on a Zeiss Axio Observer microscope equipped with a fluorescence Colibri5 light source, a second LED light source, an Axiocam 503 Mono, an Apotome 3 and the corresponding Zen 3.8 software (RRID: SCR_013672 ). ..

    Fluorescence:

    Article Title: Physiological elasticity and topography modulate in vitro prostate cancer cell behavior
    Article Snippet: .. Microscopy analysis was performed on a Zeiss Axio Observer microscope equipped with a fluorescence Colibri5 light source, a second LED light source, an Axiocam 503 Mono, an Apotome 3 and the corresponding Zen 3.8 software (RRID: SCR_013672 ). ..

    Software:

    Article Title: Physiological elasticity and topography modulate in vitro prostate cancer cell behavior
    Article Snippet: .. Microscopy analysis was performed on a Zeiss Axio Observer microscope equipped with a fluorescence Colibri5 light source, a second LED light source, an Axiocam 503 Mono, an Apotome 3 and the corresponding Zen 3.8 software (RRID: SCR_013672 ). ..

    Imaging:

    Article Title: Nuclear Architecture Related 1-Dependent H2 Reshapes Root Plasticity Through Targeting Root Meristem Cell Division Activity
    Article Snippet: .. Seedlings were immersed in 0.1% NBT, and reacted for 1 h at room temperature in the dark, followed by rinsing with distilled water and microscopic imaging (Axio Observer 3, Carl Zeiss). ..



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    ( A ) Illustration of the single molecule imaging assay, which combines microfluidics, surface immobilization and <t>TIRF</t> microscopy. ( B ) Typical field-of-view showing individual flow-aligned DNA strands (green) with MpARF2 nanoclusters (magenta) bound to the DNA templates. [MpARF2] = 1 nM. Scale bar: 5 µm. Inset: filtered Region Of Interest (ROI). ( C ) Kymograph of a nanocluster binding to DNA, illustrating the high temporal stability of a nanocluster once bound, with virtually no lateral movement. ( D ) Binding isotherm of nanoclusters, determined from the area fraction of protein covering the DNA, ϕ . Inset: binary-filtered ROIs used in coverage analysis. ( E ) Spatial distribution of binding events along individual DNA strands (solid) compared to the predicted IR7 positions (dashed). Inset: enrichment of protein on nonspecific (green) and IR7-containing (orange) DNA templates, measured as the ratio of protein intensity on DNA compared to the surrounding background. *** P <0.001; Welch’s t-test.
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    Image Search Results


    ( A ) Illustration of the single molecule imaging assay, which combines microfluidics, surface immobilization and TIRF microscopy. ( B ) Typical field-of-view showing individual flow-aligned DNA strands (green) with MpARF2 nanoclusters (magenta) bound to the DNA templates. [MpARF2] = 1 nM. Scale bar: 5 µm. Inset: filtered Region Of Interest (ROI). ( C ) Kymograph of a nanocluster binding to DNA, illustrating the high temporal stability of a nanocluster once bound, with virtually no lateral movement. ( D ) Binding isotherm of nanoclusters, determined from the area fraction of protein covering the DNA, ϕ . Inset: binary-filtered ROIs used in coverage analysis. ( E ) Spatial distribution of binding events along individual DNA strands (solid) compared to the predicted IR7 positions (dashed). Inset: enrichment of protein on nonspecific (green) and IR7-containing (orange) DNA templates, measured as the ratio of protein intensity on DNA compared to the surrounding background. *** P <0.001; Welch’s t-test.

    Journal: bioRxiv

    Article Title: Nanoclustering of a plant transcription factor enables strong yet specific DNA binding

    doi: 10.1101/2025.11.05.686732

    Figure Lengend Snippet: ( A ) Illustration of the single molecule imaging assay, which combines microfluidics, surface immobilization and TIRF microscopy. ( B ) Typical field-of-view showing individual flow-aligned DNA strands (green) with MpARF2 nanoclusters (magenta) bound to the DNA templates. [MpARF2] = 1 nM. Scale bar: 5 µm. Inset: filtered Region Of Interest (ROI). ( C ) Kymograph of a nanocluster binding to DNA, illustrating the high temporal stability of a nanocluster once bound, with virtually no lateral movement. ( D ) Binding isotherm of nanoclusters, determined from the area fraction of protein covering the DNA, ϕ . Inset: binary-filtered ROIs used in coverage analysis. ( E ) Spatial distribution of binding events along individual DNA strands (solid) compared to the predicted IR7 positions (dashed). Inset: enrichment of protein on nonspecific (green) and IR7-containing (orange) DNA templates, measured as the ratio of protein intensity on DNA compared to the surrounding background. *** P <0.001; Welch’s t-test.

    Article Snippet: Microfluidic chips were mounted on a custom built TIRF microscope ( ) and connected to a distributor valve (MUX-D-12, Elveflow) using Tygon tubing.

    Techniques: Imaging, Microscopy, Binding Assay