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Cellecta Inc custom sgrna design algorithm
Custom Sgrna Design Algorithm, supplied by Cellecta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom+sgrna+design+algorithm/custom+sgrna+design+algorithm/pm29491424-232-16-33
Average 90 stars, based on 1 article reviews
custom sgrna design algorithm - by Bioz Stars, 2026-10
90/100 stars

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CRISPR:

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1 −/− Casp11 −/− Cas9 + iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1−/−Casp11−/− Cas9+ iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Clone Assay:

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1 −/− Casp11 −/− Cas9 + iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1−/−Casp11−/− Cas9+ iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Expressing:

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1 −/− Casp11 −/− Cas9 + iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1−/−Casp11−/− Cas9+ iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Infection:

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1 −/− Casp11 −/− Cas9 + iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1−/−Casp11−/− Cas9+ iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Virus:

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1 −/− Casp11 −/− Cas9 + iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..

Article Title: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Article Snippet: .. For CRISPR sgRNA library screen, 155,164 guides targeting 19,884 mouse genes were selected by using a custom sgRNA design algorithm and the designated guides were cloned into a pooled lentiviral expression context by Cellecta, Inc. Casp1−/−Casp11−/− Cas9+ iMac were infected with a virus library titer to achieve a MOI of 0.3, with sufficient cell numbers plated to obtain a screening depth of 200 cells per sgRNA. ..



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Cellecta Inc custom sgrna design algorithm
Custom Sgrna Design Algorithm, supplied by Cellecta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom+sgrna+design+algorithm/custom+sgrna+design+algorithm/pm29491424-232-16-33
Average 90 stars, based on 1 article reviews
custom sgrna design algorithm - by Bioz Stars, 2026-10
90/100 stars
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