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INTAVIS Inc customized peptide arrays
Customized Peptide Arrays, supplied by INTAVIS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom+peptide+array/peptide+arrays/pm37463068-81-2-14
Average 90 stars, based on 1 article reviews
customized peptide arrays - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Binding Assay:

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function.
Article Snippet: .. Trapper Binding to Peptide Array Peptides covering the p21 sequence were prepared by Intavis Peptide (https:// intavispeptides.com/en/, accessed on 20 April 2017). ..

Peptide Microarray:

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function.
Article Snippet: .. Trapper Binding to Peptide Array Peptides covering the p21 sequence were prepared by Intavis Peptide (https:// intavispeptides.com/en/, accessed on 20 April 2017). ..

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function.
Article Snippet: The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). .. The purified protein segments were then incubated with a pre-designed peptide array (Intavis, Tübingen, Germany) which was loaded with peptides derived from p21. .. Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat antimouse antibody (Jackson ImmunoResearch).

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function
Article Snippet: The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). .. The purified protein segments were then incubated with a pre-designed peptide array (Intavis), which was loaded with peptides derived from p21. .. Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat anti-mouse antibody (Jackson ImmunoResearch).

Sequencing:

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function.
Article Snippet: .. Trapper Binding to Peptide Array Peptides covering the p21 sequence were prepared by Intavis Peptide (https:// intavispeptides.com/en/, accessed on 20 April 2017). ..

Synthesized:

Article Title: Inhibition of the YAP-MMB interaction and targeting NEK2 as potential therapeutic strategies for YAP-driven cancers
Article Snippet: .. μSPOT peptide arrays [ ] (CelluSpots, Intavis AG, Cologne, Germany) were synthesized using a MultiPep RSi robot (Intavis AG) on in-house produced, acid labile, amino functionalized, cellulose membrane discs containing 9-fluorenylmethyloxycarbonyl-β-alanine (Fmoc-β-Ala) linkers (average loading: 131 nmol/disc – 4 mm diameter). ..

Article Title: Proteome-wide Profiling of Asymmetric Dimethylated Arginine in Human Breast Tumors.
Article Snippet: .. The customized peptide arrays containing 214 ADMA-modified peptide sequences identified from LC-MS/MSwere synthesized by Intavis Bioanalytical Instruments AG, Germany.25 Before the in vitro methylation reaction, H2O was added to a 10 cm dish, and the peptide slides were placed on the top of the dish to allow them to hydrate in the hybridization oven for 3 h at 30 °C. .. Aliquots of 20 μg of purified, recombinant PRMT1, 4, and 6 proteins and 40 μL of radioactive S-adenosyl-L-[methyl-3H] methionine (NET155 V250UC, PerkinElmer) were mixed well in 200 μL of methylation buffer (50 mM Tris-HCl, pH 8.0, 100 mMNaCl, and 0.5mMEDTA) and incubated on the slides for 2 h at 30 °C.

Article Title: Proteome-wide Profiling of Asymmetric Di-Methylated Arginine in Human Breast Tumors
Article Snippet: .. The customized peptide arrays containing 214 ADMA-modified peptide sequences identified from LC-MS/MS were synthesized by Intavis Bioanalytical Instruments AG, Germany. ..

Produced:

Article Title: Inhibition of the YAP-MMB interaction and targeting NEK2 as potential therapeutic strategies for YAP-driven cancers
Article Snippet: .. μSPOT peptide arrays [ ] (CelluSpots, Intavis AG, Cologne, Germany) were synthesized using a MultiPep RSi robot (Intavis AG) on in-house produced, acid labile, amino functionalized, cellulose membrane discs containing 9-fluorenylmethyloxycarbonyl-β-alanine (Fmoc-β-Ala) linkers (average loading: 131 nmol/disc – 4 mm diameter). ..

Membrane:

Article Title: Inhibition of the YAP-MMB interaction and targeting NEK2 as potential therapeutic strategies for YAP-driven cancers
Article Snippet: .. μSPOT peptide arrays [ ] (CelluSpots, Intavis AG, Cologne, Germany) were synthesized using a MultiPep RSi robot (Intavis AG) on in-house produced, acid labile, amino functionalized, cellulose membrane discs containing 9-fluorenylmethyloxycarbonyl-β-alanine (Fmoc-β-Ala) linkers (average loading: 131 nmol/disc – 4 mm diameter). ..

Modification:

Article Title: Ubiquitin Interacting Motifs: Duality Between Structured and Disordered Motifs
Article Snippet: We then further purified the eluted samples by size-exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare LifeSciences, Little Chalfont, England) in PBS buffer, pH 7.4, 150 mM NaCl. .. We purchased peptide arrays from Intavis and modified the procedures for blocking and probing the arrays from ( ). ..

Blocking Assay:

Article Title: Ubiquitin Interacting Motifs: Duality Between Structured and Disordered Motifs
Article Snippet: We then further purified the eluted samples by size-exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare LifeSciences, Little Chalfont, England) in PBS buffer, pH 7.4, 150 mM NaCl. .. We purchased peptide arrays from Intavis and modified the procedures for blocking and probing the arrays from ( ). ..

In Vitro:

Article Title: Proteome-wide Profiling of Asymmetric Dimethylated Arginine in Human Breast Tumors.
Article Snippet: .. The customized peptide arrays containing 214 ADMA-modified peptide sequences identified from LC-MS/MSwere synthesized by Intavis Bioanalytical Instruments AG, Germany.25 Before the in vitro methylation reaction, H2O was added to a 10 cm dish, and the peptide slides were placed on the top of the dish to allow them to hydrate in the hybridization oven for 3 h at 30 °C. .. Aliquots of 20 μg of purified, recombinant PRMT1, 4, and 6 proteins and 40 μL of radioactive S-adenosyl-L-[methyl-3H] methionine (NET155 V250UC, PerkinElmer) were mixed well in 200 μL of methylation buffer (50 mM Tris-HCl, pH 8.0, 100 mMNaCl, and 0.5mMEDTA) and incubated on the slides for 2 h at 30 °C.

Methylation:

Article Title: Proteome-wide Profiling of Asymmetric Dimethylated Arginine in Human Breast Tumors.
Article Snippet: .. The customized peptide arrays containing 214 ADMA-modified peptide sequences identified from LC-MS/MSwere synthesized by Intavis Bioanalytical Instruments AG, Germany.25 Before the in vitro methylation reaction, H2O was added to a 10 cm dish, and the peptide slides were placed on the top of the dish to allow them to hydrate in the hybridization oven for 3 h at 30 °C. .. Aliquots of 20 μg of purified, recombinant PRMT1, 4, and 6 proteins and 40 μL of radioactive S-adenosyl-L-[methyl-3H] methionine (NET155 V250UC, PerkinElmer) were mixed well in 200 μL of methylation buffer (50 mM Tris-HCl, pH 8.0, 100 mMNaCl, and 0.5mMEDTA) and incubated on the slides for 2 h at 30 °C.

Hybridization:

Article Title: Proteome-wide Profiling of Asymmetric Dimethylated Arginine in Human Breast Tumors.
Article Snippet: .. The customized peptide arrays containing 214 ADMA-modified peptide sequences identified from LC-MS/MSwere synthesized by Intavis Bioanalytical Instruments AG, Germany.25 Before the in vitro methylation reaction, H2O was added to a 10 cm dish, and the peptide slides were placed on the top of the dish to allow them to hydrate in the hybridization oven for 3 h at 30 °C. .. Aliquots of 20 μg of purified, recombinant PRMT1, 4, and 6 proteins and 40 μL of radioactive S-adenosyl-L-[methyl-3H] methionine (NET155 V250UC, PerkinElmer) were mixed well in 200 μL of methylation buffer (50 mM Tris-HCl, pH 8.0, 100 mMNaCl, and 0.5mMEDTA) and incubated on the slides for 2 h at 30 °C.

Purification:

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function.
Article Snippet: The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). .. The purified protein segments were then incubated with a pre-designed peptide array (Intavis, Tübingen, Germany) which was loaded with peptides derived from p21. .. Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat antimouse antibody (Jackson ImmunoResearch).

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function
Article Snippet: The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). .. The purified protein segments were then incubated with a pre-designed peptide array (Intavis), which was loaded with peptides derived from p21. .. Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat anti-mouse antibody (Jackson ImmunoResearch).

Incubation:

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function.
Article Snippet: The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). .. The purified protein segments were then incubated with a pre-designed peptide array (Intavis, Tübingen, Germany) which was loaded with peptides derived from p21. .. Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat antimouse antibody (Jackson ImmunoResearch).

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function
Article Snippet: The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). .. The purified protein segments were then incubated with a pre-designed peptide array (Intavis), which was loaded with peptides derived from p21. .. Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat anti-mouse antibody (Jackson ImmunoResearch).

Derivative Assay:

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function.
Article Snippet: The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). .. The purified protein segments were then incubated with a pre-designed peptide array (Intavis, Tübingen, Germany) which was loaded with peptides derived from p21. .. Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat antimouse antibody (Jackson ImmunoResearch).

Article Title: Investigating the p21 Ubiquitin-Independent Degron Reveals a Dual Degron Module Regulating p21 Degradation and Function
Article Snippet: The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). .. The purified protein segments were then incubated with a pre-designed peptide array (Intavis), which was loaded with peptides derived from p21. .. Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat anti-mouse antibody (Jackson ImmunoResearch).



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Experimental strategy and characteristics of PB isolated from peripheral blood of 11 children 1–3 weeks after KD fever onset. A, Study overview. B–D, Analysis of single cells from 11 children with KD. B, Most PB were VH3, VH4, or VH1. C, Number of PB by isotype; IgA and IgG PB were most commonly identified. D, Of 1156 PB sequenced, 42 sets of oligoclonal PB were identified for antibody production and 15 somatically mutated IgA plasmablasts were also selected for production. Of 60 monoclonal antibodies prepared, 10 strongly bound to intracytoplasmic inclusion bodies in ciliated bronchial epithelial cells of children who died of KD using immunohistochemistry and 22 were found to bind weakly. Strongly binding antibodies were tested on an <t>animal</t> <t>virus</t> <t>peptide</t> <t>array.</t> Abbreviations: IgA, immunoglobulin A; IgG, immunoglobulin G; KD, Kawasaki disease; PB, plasmablasts; RT-PCR, reverse transcription and polymerase chain reaction; VH, variable heavy; VL, variable light.
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Experimental strategy and characteristics of PB isolated from peripheral blood of 11 children 1–3 weeks after KD fever onset. A, Study overview. B–D, Analysis of single cells from 11 children with KD. B, Most PB were VH3, VH4, or VH1. C, Number of PB by isotype; IgA and IgG PB were most commonly identified. D, Of 1156 PB sequenced, 42 sets of oligoclonal PB were identified for antibody production and 15 somatically mutated IgA plasmablasts were also selected for production. Of 60 monoclonal antibodies prepared, 10 strongly bound to intracytoplasmic inclusion bodies in ciliated bronchial epithelial cells of children who died of KD using immunohistochemistry and 22 were found to bind weakly. Strongly binding antibodies were tested on an animal virus peptide array. Abbreviations: IgA, immunoglobulin A; IgG, immunoglobulin G; KD, Kawasaki disease; PB, plasmablasts; RT-PCR, reverse transcription and polymerase chain reaction; VH, variable heavy; VL, variable light.

Journal: The Journal of Infectious Diseases

Article Title: A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease

doi: 10.1093/infdis/jiaa066

Figure Lengend Snippet: Experimental strategy and characteristics of PB isolated from peripheral blood of 11 children 1–3 weeks after KD fever onset. A, Study overview. B–D, Analysis of single cells from 11 children with KD. B, Most PB were VH3, VH4, or VH1. C, Number of PB by isotype; IgA and IgG PB were most commonly identified. D, Of 1156 PB sequenced, 42 sets of oligoclonal PB were identified for antibody production and 15 somatically mutated IgA plasmablasts were also selected for production. Of 60 monoclonal antibodies prepared, 10 strongly bound to intracytoplasmic inclusion bodies in ciliated bronchial epithelial cells of children who died of KD using immunohistochemistry and 22 were found to bind weakly. Strongly binding antibodies were tested on an animal virus peptide array. Abbreviations: IgA, immunoglobulin A; IgG, immunoglobulin G; KD, Kawasaki disease; PB, plasmablasts; RT-PCR, reverse transcription and polymerase chain reaction; VH, variable heavy; VL, variable light.

Article Snippet: A custom animal virus discovery peptide array was designed (PEPperPRINT, www.pepperprint.com ) and antibodies KD4-2H4 and KD6-2B2 tested on the array.

Techniques: Isolation, Bioprocessing, Immunohistochemistry, Binding Assay, Virus, Peptide Microarray, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Polymerase Chain Reaction

Identifying the peptide motif recognized by Kawasaki disease (KD) monoclonal antibody KD4-2H4 using animal virus peptide array and substitution array. A, Animal virus peptide array demonstrates that KD4-2H4 recognizes multiple related peptides with averaged median foreground fluorescence intensities above a cutoff value of 200; the epitope identity number from the Immune Epitope Database (www.iedb.org) is listed for each reacting peptide. B, The motif of KD4-2H4 binding identified as statistically significant by MEME bioinformatics analysis. C, Substitution matrix array of peptide AIIPDREALYQEFDEME using KD4-2H4. Preferred amino acids for binding are those on the left of the substitution matrix while nonpreferred amino acids are those on the right.

Journal: The Journal of Infectious Diseases

Article Title: A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease

doi: 10.1093/infdis/jiaa066

Figure Lengend Snippet: Identifying the peptide motif recognized by Kawasaki disease (KD) monoclonal antibody KD4-2H4 using animal virus peptide array and substitution array. A, Animal virus peptide array demonstrates that KD4-2H4 recognizes multiple related peptides with averaged median foreground fluorescence intensities above a cutoff value of 200; the epitope identity number from the Immune Epitope Database (www.iedb.org) is listed for each reacting peptide. B, The motif of KD4-2H4 binding identified as statistically significant by MEME bioinformatics analysis. C, Substitution matrix array of peptide AIIPDREALYQEFDEME using KD4-2H4. Preferred amino acids for binding are those on the left of the substitution matrix while nonpreferred amino acids are those on the right.

Article Snippet: A custom animal virus discovery peptide array was designed (PEPperPRINT, www.pepperprint.com ) and antibodies KD4-2H4 and KD6-2B2 tested on the array.

Techniques: Virus, Peptide Microarray, Fluorescence, Binding Assay