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Becton Dickinson custom bd single cell sample multiplexing kit
Custom Bd Single Cell Sample Multiplexing Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom+multiplex+kit/human+single+cell+multiplexing+kit/pm40497968-89-6-7
Average 90 stars, based on 1 article reviews
custom bd single cell sample multiplexing kit - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Single-cell RNA sequencing highlights the influence of innate and adaptive immune response mechanisms in psoriatic arthritis
Article Snippet: Cells were labeled with the BD ® Single-Cell Multiplexing Kit (BD PharmingenTM) ( ) using different sample tags 1–12 and washed twice with stain buffer (BD PharmingenTM).

Article Title: CTLA4 modulates B cell receptor signals to inhibit HBsAb secretion in chronic hepatitis B patients.
Article Snippet: Restoring B cell defects is crucial to achieve the functional cure of chronic hepatitis B virus (CHB) infection, yet the specific targets remain largely unexplored.. Our study identified that CTLA4 was highly upregulated in both peripheral and hepatic HBsAg-specific B cells from CHB patients, while effective peginterferon-α treatment could reduce the frequency of CTLA4HBsAg B cells.. Single-cell RNA-seq analysis revealed that the diminished IL-6 JAK/STAT3 and IL-2/STAT5 signaling pathways in memory B cells from CHB patients,which might contribute to the incapability of HBsAb antibody secretion.

Article Title: TIM ‐3 marks measurable residual leukemic stem cells responsible for relapse after allogeneic stem cell transplantation
Article Snippet: All cells were labeled with a BD Single-Cell Multiplexing Kit and a BD AbSeqTM antibody against TIM-3 prior to isolation.

Article Title: TIMP1 mediates astrocyte-dependent local immunosuppression in brain metastasis acting on infiltrating CD8+ T cells.
Article Snippet: Acting on Infiltrating CD8+ T Cells Neibla Priego1*, Ana de Pablos-Aragoneses1, María Perea-García1, Valentina Pieri1, Carolina Hernández-Oliver1, Laura Álvaro-Espinosa1, Andrea Rojas1, Oliva Sánchez1, Ariane Steindl1, Eduardo Caleiras2, Fernando García3, Santiago García-Martín4, Osvaldo Graña-Castro4, Sandra García-Mulero5,6, Diego Serrano7,8, Paloma Velasco-Beltrán9, Borja Jiménez-Lasheras9, Leire Egia-Mendikute9, Luise Rupp10, Antonia Stammberger10, Matthias Meinhardt11, Anas Chaachou-Charradi12, Elena Martínez-Saez12, Luca Bertero13,14, Paola Cassoni13,14, Luca Mangherini13,14, Alessia Pellerino15, Roberta Rudà15, Riccardo Soffietti16, Fatima Al-Shahrour4, Paul Saftig17, Rebeca Sanz-Pamplona5,18,19, Marc Schmitz9,20,21, Stephen J. Crocker22, Alfonso Calvo7, Asís Palazón8,23, RENACER24, and Manuel Valiente1* D ow nloaded from http://aacrjournals.org/cancerdiscovery/article-pdf/doi/10.1158/2159-8290.C D -24-0134/3514530/cd-24-0134.pdf by guest on 13 N ovem ber 2024 AACRJournals.orgOF2 | CANCER DISCOVERY XXX 2024 1Brain Metastasis Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.. 2Histopathology Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.. 3Proteomics Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.

Article Title: Parallel comparison of T cell and B cell subpopulations of adenoid hypertrophy and tonsil hypertrophy of children.
Article Snippet: Cells from each sample were labeled with sample tags (BD Human Single-Cell Multiplexing Kit) following themanufacturer’s protocol.

Article Title: Characterization of human CMV-specific CD8 + T cells using multi-layer single-cell omics.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD158b (clone CH-L), custom AbSeq DNA barcode BD Biosciences Cat# 559783, RRID:AB_397324 PE mouse anti-human CD45 (clone HI30) BioLegend Cat# 304039, RRID:AB_2562057 BV421 mouse anti-human CD3 (clone UCHT1) BD Biosciences Cat# 562426, RRID:AB_11152082 Chemicals, peptides, and recombinant proteins FcR Block Reagent, human Miltenyi Cat# 130-059-901 Invitrogen UltraPure Herring Sperm DNA Solution (sheared) Thermo Fischer Cat# 15634017 D-Biotin Thermo Fischer Cat# B20656 AMPure XP Bead-Based Reagent Beckman Coulter Cat# A63881 BD Pharmingen Stain Buffer (FBS) Becton Dickinson Cat# 554656 Fetal bovine serum PAN Biotec Cat# 3302 RPMI 1640 medium Gibo Cat# 21875034 PBS, pH 7.4 Gibco Cat# 15374875 Critical commercial assays Human CD8+ T cell isolation kit Miltenyi Cat# 130-096-495 BD Rhapsody cDNA Kit Becton Dickinson Cat# 633773 BD Rhapsody WTA Amplification Kit Becton Dickinson Cat# 633801 BD Rhapsody TCR/BCR Amplification Kit Becton Dickinson Cat# 665345 BD Rhapsody Enhanced Cartridge Reagent Kit Becton Dickinson Cat# 664887 BD Rhapsody Cartridge Kit Becton Dickinson Cat# 633733 BD AbSeq Immune Discovery Panel Becton Dickinson Cat# 625970 BD Single-Cell Multiplexing Kit, Human Sample Tag (12) Component Becton Dickinson Cat# 633781 BD Flex Single-Cell Multiplexing Kit C, Flex Sample Tag 13-18 Becton Dickinson Cat# 633851 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation, dilution 1:1000 Thermo Fischer Cat# L34975 NovaSeq 6000 S4 Reagent Kit v1.5 (300 cycles) Illumina Cat# 20028312 Deposited data Single-cell multi-omics raw data This paper EGA: EGA50000000594 Software and algorithms FlowJo BD Biosciences https://flowjo.com, RRID:SCR_008520 Bcl2fastq2 v2.20 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html, RRID:SCR_015058 STAR v2.6.1b Dobin et al.40 https://github.com/alexdobin/STAR/, RRID:SCR_004463 Cutadapt v1.16 Martin41 https://github.com/marcelm/cutadapt, RRID:SCR_011841 Dropseq-tools Broad Institute https://github.com/broadinstitute/Drop- seq/ (Continued on next page) e1 Cell Reports Methods 5, 101085, July 21, 2025 Report ll OPEN ACCESS

Article Title: Co-culture of human AT2 cells with fibroblasts reveals a MUC5B phenotype: insights from an organoid model.
Article Snippet: Samples were individually labelled using the Human Single-Cell Multiplexing Kit (BD, Cat. 633781) and subsequently pooled.

Single Cell:

Article Title: Insulin-Degrading Enzyme Regulates mRNA Processing and May Interact with the CCR4-NOT Complex.
Article Snippet: The cell concentration and viability were determined with a BD Rhapsody Scanner. .. Cell barcoding was performed using the Custom BD Single Cell Sample Multiplexing Kit (No. 626545, Becton Dickinson, Le Pont de Claix, France) after the same number of cells was pooled for each genotype. ..

Multiplexing:

Article Title: Insulin-Degrading Enzyme Regulates mRNA Processing and May Interact with the CCR4-NOT Complex.
Article Snippet: The cell concentration and viability were determined with a BD Rhapsody Scanner. .. Cell barcoding was performed using the Custom BD Single Cell Sample Multiplexing Kit (No. 626545, Becton Dickinson, Le Pont de Claix, France) after the same number of cells was pooled for each genotype. ..



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