Journal: PLoS ONE
Article Title: The Antarctic Krill Euphausia superba Shows Diurnal Cycles of Transcription under Natural Conditions
doi: 10.1371/journal.pone.0068652
Figure Lengend Snippet: A) Schematic representation of the daily distribution of metabolic processes resulting from the transcriptional signature of several differentially expressed genes throughout the 24-hour cycle. The different metabolic processes are indicated by gradiently colored arrows showing the time of the day corresponding to the higher expression levels of gene groups. The lengths of the arrows and darker colors indicate, respectively, intervals and peaks of expression. Local times are indicated at the bottom of the figure where an indicative representation of light intensity is also shown. The breakdown of energy-yielding nutrients (glycolysis, the Krebs cycle and the electron transport chain) and energy storage pathways (glycogen synthesis and fatty acid synthesis) are specifically activated in the early morning, while glycogen mobilization, gluconeogenesis and fatty acids catabolism are used as a stored energy source in the evening and throughout the night. B) Gene expression profiles of transcripts involved in energetic and metabolic processes are represented. The color of each gene corresponds to the metabolic process in which it is involved. Dashed lines indicate differentially expressed genes identified by a one-way ANOVA test, while solid lines represent differentially expressed genes characterized by a sinusoidal expression patterns detected by CircWaveBatch analysis. Microarray expression values are represented as log 2 mean ± standard deviation. Genes are represented with the following abbreviations: fructose 1,6-bisphosphatase ( FB ), glyceraldehyde-3-phosphate dehydrogenase ( GPD ), pyruvate kinase ( PK ), 6-phosphofructokinase ( 6P ), citrate synthase ( CS ), ATP synthase b ( ATP ), succinate dehydrogenase type C ( SD ), isocitrate dehydrogenase [NAD] subunit beta mitochondrial ( ID ), Acetyl-CoA acetyltransferase ( ACA ), short-chain specific acyl-CoA dehydrogenase ( ACD ), Acetyl-CoA carboxylase ( ACC ), ATP citrate lyase subunit beta ( ACL ), glycogen debranching enzyme-like ( GDE ), glycogen synthase ( GS ), and Glycogenin-1 ( G ).
Article Snippet: Probes were designed using the Agilent eArray Custom Microarray Design Service ( https://earray.chem.agilent.com/earray/index.jsp ), which applies proprietary prediction algorithms to design 60 mer oligo-probes.
Techniques: Expressing, Microarray, Standard Deviation
![A) Schematic representation of the daily distribution of metabolic processes resulting from the transcriptional signature of several differentially expressed genes throughout the 24-hour cycle. The different metabolic processes are indicated by gradiently colored arrows showing the time of the day corresponding to the higher expression levels of gene groups. The lengths of the arrows and darker colors indicate, respectively, intervals and peaks of expression. Local times are indicated at the bottom of the figure where an indicative representation of light intensity is also shown. The breakdown of energy-yielding nutrients (glycolysis, the Krebs cycle and the electron transport chain) and energy storage pathways (glycogen synthesis and fatty acid synthesis) are specifically activated in the early morning, while glycogen mobilization, gluconeogenesis and fatty acids catabolism are used as a stored energy source in the evening and throughout the night. B) Gene expression profiles of transcripts involved in energetic and metabolic processes are represented. The color of each gene corresponds to the metabolic process in which it is involved. Dashed lines indicate differentially expressed genes identified by a one-way ANOVA test, while solid lines represent differentially expressed genes characterized by a sinusoidal expression patterns detected by CircWaveBatch analysis. <t>Microarray</t> expression values are represented as log 2 mean ± standard deviation. Genes are represented with the following abbreviations: fructose 1,6-bisphosphatase ( FB ), glyceraldehyde-3-phosphate dehydrogenase ( GPD ), pyruvate kinase ( PK ), 6-phosphofructokinase ( 6P ), citrate synthase ( CS ), ATP synthase b ( ATP ), succinate dehydrogenase type C ( SD ), isocitrate dehydrogenase [NAD] subunit beta mitochondrial ( ID ), Acetyl-CoA acetyltransferase ( ACA ), short-chain specific acyl-CoA dehydrogenase ( ACD ), Acetyl-CoA carboxylase ( ACC ), ATP citrate lyase subunit beta ( ACL ), glycogen debranching enzyme-like ( GDE ), glycogen synthase ( GS ), and Glycogenin-1 ( G ).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4250/pmc03714250/pmc03714250__pone.0068652.g002.jpg)