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harmony analysis software  (Revvity)


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    Structured Review

    Revvity harmony analysis software
    Harmony Analysis Software, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+image+analysis+software/Harmony+High-Content+Imaging+and+Analysis+Software/pmc10216787-48-8-13
    Average 94 stars, based on 329 article reviews
    harmony analysis software - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Endonucleosis mediates internalization of cytoplasm into the nucleus in senescent cells
    Article Snippet: For chromosomal ploidy evaluation, isolated nuclei were seeded onto Superfrost® Plus glass microscope slides, fixed with 70% EtOH and stained with 50μg/ml propidium iodide (Sigma-Aldrich, P4864) for 30 minutes. .. After washing with PBS and mounting with Mowiol® 4-88 Reagent, PI images were collected using Operetta (Perkin Elmer) high content microscope and PI intensity data were evaluated using the Harmony Software 4.1 of PerkinElmer. .. Tissue cryosections and isolated nuclei on slides labeled with immunofluorescence were captured with a 20x or 40x lens (Olympus–Shinjuku City, Tokyo, Japan) with the Operetta High Content Screening Microscope (HCSM) (PerkinElmer, Waltham, MA, USA) in wide field and analyzed using Harmony software 4.1 with PhenoLOGIC (PerkinElmer) or the open source software FIJI/Image J.

    Software:

    Article Title: Endonucleosis mediates internalization of cytoplasm into the nucleus in senescent cells
    Article Snippet: For chromosomal ploidy evaluation, isolated nuclei were seeded onto Superfrost® Plus glass microscope slides, fixed with 70% EtOH and stained with 50μg/ml propidium iodide (Sigma-Aldrich, P4864) for 30 minutes. .. After washing with PBS and mounting with Mowiol® 4-88 Reagent, PI images were collected using Operetta (Perkin Elmer) high content microscope and PI intensity data were evaluated using the Harmony Software 4.1 of PerkinElmer. .. Tissue cryosections and isolated nuclei on slides labeled with immunofluorescence were captured with a 20x or 40x lens (Olympus–Shinjuku City, Tokyo, Japan) with the Operetta High Content Screening Microscope (HCSM) (PerkinElmer, Waltham, MA, USA) in wide field and analyzed using Harmony software 4.1 with PhenoLOGIC (PerkinElmer) or the open source software FIJI/Image J.

    Article Title: Adaptation to ARF6-depletion in KRAS-driven PDAC is abolished by targeting TLR2
    Article Snippet: Measurements were taken in the form of images using the Operetta High Content Screening System (Perkin Elmer, see section 3.2.6) at 37 °C and 5% CO 2 . .. Quantification of spheroid area was done using the in-built Harmony analysis software (Perkin Elmer). ..

    Article Title: Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas
    Article Snippet: .. The Harmony software (PerkinElmer) on the Operetta was used to evaluate cell speed, accumulated distance and displacement, and calculate the overall invasion and migration area (n = 5). ..

    Article Title: Adaptation to ARF6-depletion in KRAS-driven PDAC is abolished by targeting TLR2
    Article Snippet: Samples were then imaged using the Operetta High Content Screening System (Perkin Elmer) at 20x magnification and at appropriate wavelengths for DAPI (Ex: 405 nm /Em: 461 nm). .. Since individual images only covered a fraction of the entire membrane, the entire area was captured using multiple images and stitched using the in-built Harmony software (Perkin Elmer). .. Post-acquisition image analysis was performed on ImageJ (FIJI).

    Article Title: Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin
    Article Snippet: .. Images were acquired and analyzed using the high-content imaging system Operetta® and Harmony® software (PerkinElmer, Waltham, MA, USA). .. The complete cDNA of human CRYAB was cloned into an AAV6 vector (AAVpro® Helper Free System, Takara Bio) in frame with the tdTomato gene (AAV6-CMV-CRYAB-P2A-tdTomato) through a self-cleaving P2A peptide.

    Article Title: Adaptation to ARF6-depletion in KRAS-driven PDAC is abolished by targeting TLR2
    Article Snippet: Following 8 hours to allow cells to become adherent, cells were stained with Hoechst 33342 (Life Technologies #H3570) and imaged live for 10 hours using the Operetta High Content Screening System (Perkin Elmer, see section 3.2.6) at 37 °C and 5% CO 2 at intervals of 20 mins. .. Post imaging, cell tracking and quantification of distance and displacement per cell was calculated using the in-built Harmony analysis software (Perkin Elmer). ..

    Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
    Article Snippet: Immunofluorescent stainings were imaged using a high-throughput confocal microscope equipped with a chamber with controlled temperature and CO 2 concentration, for live cell imaging (Opera Phenix High Content Screening System; Perkin Elmer). .. For quantitative analysis of immunocytochemistry staining, images were analyzed using Harmony High-Content Imaging and Analysis Software (v. 4.9, Opera Phenix; Perkin Elmer). ..

    Migration:

    Article Title: Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas
    Article Snippet: .. The Harmony software (PerkinElmer) on the Operetta was used to evaluate cell speed, accumulated distance and displacement, and calculate the overall invasion and migration area (n = 5). ..

    Membrane:

    Article Title: Adaptation to ARF6-depletion in KRAS-driven PDAC is abolished by targeting TLR2
    Article Snippet: Samples were then imaged using the Operetta High Content Screening System (Perkin Elmer) at 20x magnification and at appropriate wavelengths for DAPI (Ex: 405 nm /Em: 461 nm). .. Since individual images only covered a fraction of the entire membrane, the entire area was captured using multiple images and stitched using the in-built Harmony software (Perkin Elmer). .. Post-acquisition image analysis was performed on ImageJ (FIJI).

    Imaging:

    Article Title: Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin
    Article Snippet: .. Images were acquired and analyzed using the high-content imaging system Operetta® and Harmony® software (PerkinElmer, Waltham, MA, USA). .. The complete cDNA of human CRYAB was cloned into an AAV6 vector (AAVpro® Helper Free System, Takara Bio) in frame with the tdTomato gene (AAV6-CMV-CRYAB-P2A-tdTomato) through a self-cleaving P2A peptide.

    Article Title: Adaptation to ARF6-depletion in KRAS-driven PDAC is abolished by targeting TLR2
    Article Snippet: Following 8 hours to allow cells to become adherent, cells were stained with Hoechst 33342 (Life Technologies #H3570) and imaged live for 10 hours using the Operetta High Content Screening System (Perkin Elmer, see section 3.2.6) at 37 °C and 5% CO 2 at intervals of 20 mins. .. Post imaging, cell tracking and quantification of distance and displacement per cell was calculated using the in-built Harmony analysis software (Perkin Elmer). ..

    Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
    Article Snippet: Immunofluorescent stainings were imaged using a high-throughput confocal microscope equipped with a chamber with controlled temperature and CO 2 concentration, for live cell imaging (Opera Phenix High Content Screening System; Perkin Elmer). .. For quantitative analysis of immunocytochemistry staining, images were analyzed using Harmony High-Content Imaging and Analysis Software (v. 4.9, Opera Phenix; Perkin Elmer). ..

    Cell Tracking Assay:

    Article Title: Adaptation to ARF6-depletion in KRAS-driven PDAC is abolished by targeting TLR2
    Article Snippet: Following 8 hours to allow cells to become adherent, cells were stained with Hoechst 33342 (Life Technologies #H3570) and imaged live for 10 hours using the Operetta High Content Screening System (Perkin Elmer, see section 3.2.6) at 37 °C and 5% CO 2 at intervals of 20 mins. .. Post imaging, cell tracking and quantification of distance and displacement per cell was calculated using the in-built Harmony analysis software (Perkin Elmer). ..

    Immunocytochemistry:

    Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
    Article Snippet: Immunofluorescent stainings were imaged using a high-throughput confocal microscope equipped with a chamber with controlled temperature and CO 2 concentration, for live cell imaging (Opera Phenix High Content Screening System; Perkin Elmer). .. For quantitative analysis of immunocytochemistry staining, images were analyzed using Harmony High-Content Imaging and Analysis Software (v. 4.9, Opera Phenix; Perkin Elmer). ..

    Staining:

    Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
    Article Snippet: Immunofluorescent stainings were imaged using a high-throughput confocal microscope equipped with a chamber with controlled temperature and CO 2 concentration, for live cell imaging (Opera Phenix High Content Screening System; Perkin Elmer). .. For quantitative analysis of immunocytochemistry staining, images were analyzed using Harmony High-Content Imaging and Analysis Software (v. 4.9, Opera Phenix; Perkin Elmer). ..



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