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custom-built multiphoton fluorescence microscope ultima  (Bruker Corporation)


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    Structured Review

    Bruker Corporation custom-built multiphoton fluorescence microscope ultima
    Custom Built Multiphoton Fluorescence Microscope Ultima, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+built+multiphoton+microscope/custom+built+multiphoton+fluorescence+microscope+ultima/pmc12006760-142-7-11
    Average 90 stars, based on 1 article reviews
    custom-built multiphoton fluorescence microscope ultima - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Autofluorescence lifetime imaging classifies human lymphocyte activation and subtype
    Article Snippet: For imaging, B cells and NK cells were plated 1 hour before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker & Hickl GbH, Berlin, Germany). .. The laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, USA) was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1040 nm excitation for PerCP.

    Article Title: Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.
    Article Snippet: There is an ongoing need to do more with less and provide highly multiplexed analysis from limited sample volumes.. Improved “sample sparing” assays would have a broad impact across pediatric and other rare sample type studies in addition to enabling sequential sampling.. This capability would advance both clinical and basic research applications.

    Article Title: Autofluorescence lifetime imaging classifies human B and NK cell activation state
    Article Snippet: For imaging, cells were plated 1 h before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker and Hickl GbH, Berlin, Germany). .. The femtosecond-pulsed laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, United States) with 80 MHz pulse repetition rate was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1,040 nm excitation for PerCP.

    Article Title: Extracellular pH affects the fluorescence lifetimes of metabolic co-factors
    Article Snippet: .. The solutions of cholesterol oxidase and FAD were dissolved in buffer and imaged at a concentration of 0.81 μ mol / L . All samples were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) equipped with time correlated single photon counting electronics (SPC-150, Becker & Hickl GmbH, Berlin, Germany). ..

    Microscopy:

    Article Title: Autofluorescence lifetime imaging classifies human lymphocyte activation and subtype
    Article Snippet: For imaging, B cells and NK cells were plated 1 hour before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker & Hickl GbH, Berlin, Germany). .. The laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, USA) was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1040 nm excitation for PerCP.

    Article Title: Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.
    Article Snippet: There is an ongoing need to do more with less and provide highly multiplexed analysis from limited sample volumes.. Improved “sample sparing” assays would have a broad impact across pediatric and other rare sample type studies in addition to enabling sequential sampling.. This capability would advance both clinical and basic research applications.

    Article Title: Autofluorescence lifetime imaging classifies human B and NK cell activation state
    Article Snippet: For imaging, cells were plated 1 h before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker and Hickl GbH, Berlin, Germany). .. The femtosecond-pulsed laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, United States) with 80 MHz pulse repetition rate was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1,040 nm excitation for PerCP.

    Article Title: Extracellular pH affects the fluorescence lifetimes of metabolic co-factors
    Article Snippet: .. The solutions of cholesterol oxidase and FAD were dissolved in buffer and imaged at a concentration of 0.81 μ mol / L . All samples were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) equipped with time correlated single photon counting electronics (SPC-150, Becker & Hickl GmbH, Berlin, Germany). ..

    Counting:

    Article Title: Autofluorescence lifetime imaging classifies human lymphocyte activation and subtype
    Article Snippet: For imaging, B cells and NK cells were plated 1 hour before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker & Hickl GbH, Berlin, Germany). .. The laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, USA) was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1040 nm excitation for PerCP.

    Article Title: Autofluorescence lifetime imaging classifies human B and NK cell activation state
    Article Snippet: For imaging, cells were plated 1 h before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker and Hickl GbH, Berlin, Germany). .. The femtosecond-pulsed laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, United States) with 80 MHz pulse repetition rate was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1,040 nm excitation for PerCP.

    Imaging:

    Article Title: Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.
    Article Snippet: There is an ongoing need to do more with less and provide highly multiplexed analysis from limited sample volumes.. Improved “sample sparing” assays would have a broad impact across pediatric and other rare sample type studies in addition to enabling sequential sampling.. This capability would advance both clinical and basic research applications.

    Concentration Assay:

    Article Title: Extracellular pH affects the fluorescence lifetimes of metabolic co-factors
    Article Snippet: .. The solutions of cholesterol oxidase and FAD were dissolved in buffer and imaged at a concentration of 0.81 μ mol / L . All samples were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) equipped with time correlated single photon counting electronics (SPC-150, Becker & Hickl GmbH, Berlin, Germany). ..

    other:

    Article Title: Microphysiological head and neck cancer model identifies novel role of lymphatically secreted monocyte migration inhibitory factor in cancer cell migration and metabolism.
    Article Snippet: Regional metastasis of head and neck cancer (HNC) is prevalent (approximately 50% of patients at diagnosis), yet the underlying drivers and mechanisms of lymphatic spread remain unclear.. The complex tumor microenvironment (TME) of HNC plays a crucial role in disease maintenance and progression; however, the contribution of the lymphatics remains underexplored.. We created a primary patient cell derived microphysiological system that incorporates cancer-associated-fibroblasts from patients with HNC alongside a HNC tumor spheroid and a lymphatic microvessel to create an in vitro TME platform to investigate metastasis.



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